Osteoarthritic Synovial Fluid Modulates Cell Phenotype and Metabolic Behavior In Vitro
Synovial fluid holds a population of mesenchymal stem cells (MSC) that could be used for clinical treatment. Our goal was to characterize the inflammatory and metabolomic profile of the synovial fluid from osteoarthritic patients and to identify its modulatory effect on synovial fluid cells. Synovia...
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| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2019-01-01
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| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2019/8169172 |
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| author | Eduardo Branco de Sousa Gilson Costa dos Santos Junior Ramon Pinheiro Aguiar Rafaela da Costa Sartore Ana Carolina Leal de Oliveira Fabio Ceneviva Lacerda Almeida Vivaldo Moura Neto Diego Pinheiro Aguiar |
| author_facet | Eduardo Branco de Sousa Gilson Costa dos Santos Junior Ramon Pinheiro Aguiar Rafaela da Costa Sartore Ana Carolina Leal de Oliveira Fabio Ceneviva Lacerda Almeida Vivaldo Moura Neto Diego Pinheiro Aguiar |
| author_sort | Eduardo Branco de Sousa |
| collection | DOAJ |
| description | Synovial fluid holds a population of mesenchymal stem cells (MSC) that could be used for clinical treatment. Our goal was to characterize the inflammatory and metabolomic profile of the synovial fluid from osteoarthritic patients and to identify its modulatory effect on synovial fluid cells. Synovial fluid was collected from non-OA and OA patients, which was centrifuged to isolate cells. Cells were cultured for 21 days, characterized with specific markers for MSC, and exposed to a specific cocktail to induce chondrogenic, osteogenic, and adipogenic differentiation. Then, we performed a MTT assay exposing SF cells from non-OA and OA patients to a medium containing non-OA and OA synovial fluid. Synovial fluid from non-OA and OA patients was submitted to ELISA to evaluate BMP-2, BMP-4, IL-6, IL-10, TNF-α, and TGF-β1 concentrations and to a metabolomic evaluation using 1H-NMR. Synovial fluid cells presented spindle-shaped morphology in vitro. Samples from OA patients formed a higher number of colonies than the ones from non-OA patients. After 21 days, the colony-forming cells from OA patients differentiated into the three mesenchymal cell lineages, under the appropriated induction protocols. Synovial fluid cells increased its metabolic activity after being exposed to the OA synovial fluid. ELISA assay showed that OA synovial fluid samples presented higher concentration of IL-10 and TGF-β1 than the non-OA, while the NMR showed that OA synovial fluid presents higher concentrations of glucose and glycerol. In conclusion, SFC activity is modulated by OA synovial fluid, which presents higher concentration of IL-10, TGF-β, glycerol, and glucose. |
| format | Article |
| id | doaj-art-f95f4b2844f046f988cf31f41b8583fd |
| institution | Kabale University |
| issn | 1687-966X 1687-9678 |
| language | English |
| publishDate | 2019-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-f95f4b2844f046f988cf31f41b8583fd2025-02-03T05:58:16ZengWileyStem Cells International1687-966X1687-96782019-01-01201910.1155/2019/81691728169172Osteoarthritic Synovial Fluid Modulates Cell Phenotype and Metabolic Behavior In VitroEduardo Branco de Sousa0Gilson Costa dos Santos Junior1Ramon Pinheiro Aguiar2Rafaela da Costa Sartore3Ana Carolina Leal de Oliveira4Fabio Ceneviva Lacerda Almeida5Vivaldo Moura Neto6Diego Pinheiro Aguiar7Research Division, National Institute of Traumatology and Orthopedics Jamil Haddad, Rio de Janeiro, RJ, BrazilCenter of Structural Biology and Bioimaging I (CENABIO I), Federal University of Rio de Janeiro, Rio de Janeiro, BrazilCenter of Structural Biology and Bioimaging I (CENABIO I), Federal University of Rio de Janeiro, Rio de Janeiro, BrazilResearch Division, National Institute of Traumatology and Orthopedics Jamil Haddad, Rio de Janeiro, RJ, BrazilResearch Division, National Institute of Traumatology and Orthopedics Jamil Haddad, Rio de Janeiro, RJ, BrazilCenter of Structural Biology and Bioimaging I (CENABIO I), Federal University of Rio de Janeiro, Rio de Janeiro, BrazilProgram of Cell and Developmental Biology, Institute of Biomedical Sciences, Federal University of Rio de Janeiro, Rio de Janeiro, RJ, BrazilResearch Division, National Institute of Traumatology and Orthopedics Jamil Haddad, Rio de Janeiro, RJ, BrazilSynovial fluid holds a population of mesenchymal stem cells (MSC) that could be used for clinical treatment. Our goal was to characterize the inflammatory and metabolomic profile of the synovial fluid from osteoarthritic patients and to identify its modulatory effect on synovial fluid cells. Synovial fluid was collected from non-OA and OA patients, which was centrifuged to isolate cells. Cells were cultured for 21 days, characterized with specific markers for MSC, and exposed to a specific cocktail to induce chondrogenic, osteogenic, and adipogenic differentiation. Then, we performed a MTT assay exposing SF cells from non-OA and OA patients to a medium containing non-OA and OA synovial fluid. Synovial fluid from non-OA and OA patients was submitted to ELISA to evaluate BMP-2, BMP-4, IL-6, IL-10, TNF-α, and TGF-β1 concentrations and to a metabolomic evaluation using 1H-NMR. Synovial fluid cells presented spindle-shaped morphology in vitro. Samples from OA patients formed a higher number of colonies than the ones from non-OA patients. After 21 days, the colony-forming cells from OA patients differentiated into the three mesenchymal cell lineages, under the appropriated induction protocols. Synovial fluid cells increased its metabolic activity after being exposed to the OA synovial fluid. ELISA assay showed that OA synovial fluid samples presented higher concentration of IL-10 and TGF-β1 than the non-OA, while the NMR showed that OA synovial fluid presents higher concentrations of glucose and glycerol. In conclusion, SFC activity is modulated by OA synovial fluid, which presents higher concentration of IL-10, TGF-β, glycerol, and glucose.http://dx.doi.org/10.1155/2019/8169172 |
| spellingShingle | Eduardo Branco de Sousa Gilson Costa dos Santos Junior Ramon Pinheiro Aguiar Rafaela da Costa Sartore Ana Carolina Leal de Oliveira Fabio Ceneviva Lacerda Almeida Vivaldo Moura Neto Diego Pinheiro Aguiar Osteoarthritic Synovial Fluid Modulates Cell Phenotype and Metabolic Behavior In Vitro Stem Cells International |
| title | Osteoarthritic Synovial Fluid Modulates Cell Phenotype and Metabolic Behavior In Vitro |
| title_full | Osteoarthritic Synovial Fluid Modulates Cell Phenotype and Metabolic Behavior In Vitro |
| title_fullStr | Osteoarthritic Synovial Fluid Modulates Cell Phenotype and Metabolic Behavior In Vitro |
| title_full_unstemmed | Osteoarthritic Synovial Fluid Modulates Cell Phenotype and Metabolic Behavior In Vitro |
| title_short | Osteoarthritic Synovial Fluid Modulates Cell Phenotype and Metabolic Behavior In Vitro |
| title_sort | osteoarthritic synovial fluid modulates cell phenotype and metabolic behavior in vitro |
| url | http://dx.doi.org/10.1155/2019/8169172 |
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