The Immunotherapeutic Effect of SIRPα-Silenced DCs against Cervical Cancer
Signal regulatory protein α (SIRPα), a transmembrane protein that is predominantly expressed in dendritic cells (DCs) or macrophages, interacts with CD47 that is overexpressed in almost all types of tumor cells. The interaction between SIRPα and CD47 leads to a negative signal that prevents the phen...
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| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2020-01-01
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| Series: | Journal of Immunology Research |
| Online Access: | http://dx.doi.org/10.1155/2020/1705187 |
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| author | Xiaojie Li Wenying Zhou Yanlan Liang Changzhi Xu Zhizhi Xie Jiayin Liang Bo Hu |
| author_facet | Xiaojie Li Wenying Zhou Yanlan Liang Changzhi Xu Zhizhi Xie Jiayin Liang Bo Hu |
| author_sort | Xiaojie Li |
| collection | DOAJ |
| description | Signal regulatory protein α (SIRPα), a transmembrane protein that is predominantly expressed in dendritic cells (DCs) or macrophages, interacts with CD47 that is overexpressed in almost all types of tumor cells. The interaction between SIRPα and CD47 leads to a negative signal that prevents the phenotypic and functional maturation of DC and inhibits phagocytosis. The SIRPα knockdown in DCs that were pulsed with a modified HPV16E7 (HPV16mE7) protein with enhanced antigenicity and reduced transformation activity results in increased cytokine (TNF-α/IL-12/IL-6) secretion, IFN-γ secretion by T lymphocytes, and in vitro/in vivo tumoricidal activity against cervical cancer cells. Taken together, these results suggest that SIRPα-silenced DC vaccination presented potential therapeutic implications against cervical cancer. |
| format | Article |
| id | doaj-art-f93b19cf48004736a646e9417bea4479 |
| institution | Kabale University |
| issn | 2314-8861 2314-7156 |
| language | English |
| publishDate | 2020-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Immunology Research |
| spelling | doaj-art-f93b19cf48004736a646e9417bea44792025-02-03T06:06:43ZengWileyJournal of Immunology Research2314-88612314-71562020-01-01202010.1155/2020/17051871705187The Immunotherapeutic Effect of SIRPα-Silenced DCs against Cervical CancerXiaojie Li0Wenying Zhou1Yanlan Liang2Changzhi Xu3Zhizhi Xie4Jiayin Liang5Bo Hu6Department of Laboratory Medicine, The Third Affiliated Hospital, Sun Yat-sen University, Guangzhou 510630, ChinaDepartment of Laboratory Medicine, The Third Affiliated Hospital, Sun Yat-sen University, Guangzhou 510630, ChinaDepartment of Laboratory Medicine, The Third Affiliated Hospital, Sun Yat-sen University, Guangzhou 510630, ChinaDepartment of Laboratory Medicine, The Third Affiliated Hospital, Sun Yat-sen University, Guangzhou 510630, ChinaDepartment of Laboratory Medicine, The Third Affiliated Hospital, Sun Yat-sen University, Guangzhou 510630, ChinaDepartment of Laboratory Medicine, The Third Affiliated Hospital, Sun Yat-sen University, Guangzhou 510630, ChinaDepartment of Laboratory Medicine, The Third Affiliated Hospital, Sun Yat-sen University, Guangzhou 510630, ChinaSignal regulatory protein α (SIRPα), a transmembrane protein that is predominantly expressed in dendritic cells (DCs) or macrophages, interacts with CD47 that is overexpressed in almost all types of tumor cells. The interaction between SIRPα and CD47 leads to a negative signal that prevents the phenotypic and functional maturation of DC and inhibits phagocytosis. The SIRPα knockdown in DCs that were pulsed with a modified HPV16E7 (HPV16mE7) protein with enhanced antigenicity and reduced transformation activity results in increased cytokine (TNF-α/IL-12/IL-6) secretion, IFN-γ secretion by T lymphocytes, and in vitro/in vivo tumoricidal activity against cervical cancer cells. Taken together, these results suggest that SIRPα-silenced DC vaccination presented potential therapeutic implications against cervical cancer.http://dx.doi.org/10.1155/2020/1705187 |
| spellingShingle | Xiaojie Li Wenying Zhou Yanlan Liang Changzhi Xu Zhizhi Xie Jiayin Liang Bo Hu The Immunotherapeutic Effect of SIRPα-Silenced DCs against Cervical Cancer Journal of Immunology Research |
| title | The Immunotherapeutic Effect of SIRPα-Silenced DCs against Cervical Cancer |
| title_full | The Immunotherapeutic Effect of SIRPα-Silenced DCs against Cervical Cancer |
| title_fullStr | The Immunotherapeutic Effect of SIRPα-Silenced DCs against Cervical Cancer |
| title_full_unstemmed | The Immunotherapeutic Effect of SIRPα-Silenced DCs against Cervical Cancer |
| title_short | The Immunotherapeutic Effect of SIRPα-Silenced DCs against Cervical Cancer |
| title_sort | immunotherapeutic effect of sirpα silenced dcs against cervical cancer |
| url | http://dx.doi.org/10.1155/2020/1705187 |
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