Study on the role of activation of TGF-β pathway by ASPN in the occurrence and development of keloid and the underlying mechanisms
[Objective] To investigate the role of ASPN in the development of keloid and the underlying mechanisms. [Methods] Immunofluorescence staining was used to assess the expression levels of ASPN in keloid of keloid patients and normal scar. WB and qRT-PCR were used to assess the activity of TGF-β pathwa...
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| Format: | Article |
| Language: | zho |
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editoiral office of Journal of Diagnosis and Therapy on Dermato-venereology
2025-01-01
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| Series: | Pifu-xingbing zhenliaoxue zazhi |
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| Online Access: | http://pfxbzlx.gdvdc.com/EN/10.3969/j.issn.1674-8468.2025.01.002 |
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| _version_ | 1825199624853389312 |
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| author | XU Xueyan CHEN Junyi YAO Liuyi DENG Chengcheng |
| author_facet | XU Xueyan CHEN Junyi YAO Liuyi DENG Chengcheng |
| author_sort | XU Xueyan |
| collection | DOAJ |
| description | [Objective] To investigate the role of ASPN in the development of keloid and the underlying mechanisms. [Methods] Immunofluorescence staining was used to assess the expression levels of ASPN in keloid of keloid patients and normal scar. WB and qRT-PCR were used to assess the activity of TGF-β pathway and expression levels of α-SMA、Collagen Ⅰ/Ⅲ, COMP and POSTN in fibroblasts with overexpression of ASPN, ASPN-treated fibroblasts and Keloid fibroblasts with ASPN knockdown. Moreover, wound healing was observed in C57BL/6J mice treated with or without ASPN at 0 d, 3 d, 7 d, and 14 d after wound. The expression levels of α-SMA、Collagen Ⅰ/Ⅲ and TGF-β in the wounded skin were assessed by immunofluorescence staining 14 days after wound. [Results] ASPN was highly expressed in keloid fibroblasts. The expression levels of α-SMA、Collagen Ⅰ/Ⅲ、COMP and POSTN were significantly increased, and the TGF-β signaling pathway was activated in fibroblasts after either overexpression of or stimulation with ASPN (P<0.05). In contrast, knockdown of ASPN significantly decreased the expression levels of α-SMA、Collagen Ⅰ/Ⅲ, COMP and POSTN and inhibited TGF-β pathway in fibroblasts from keloids (P<0.05). In addition, ASPN significantly increased the expression levels of p-SMAD2、α-SMA and Collagen Ⅰ and activated TGF-β signaling pathway in the wound area (P<0.05 vs. controls), without altering the wound healing process. [Conclusion] ASPN promotes the development of keloid by activating TGF-β signaling pathway. |
| format | Article |
| id | doaj-art-f92a952f4fa049549dfb62980cb71d2d |
| institution | Kabale University |
| issn | 1674-8468 |
| language | zho |
| publishDate | 2025-01-01 |
| publisher | editoiral office of Journal of Diagnosis and Therapy on Dermato-venereology |
| record_format | Article |
| series | Pifu-xingbing zhenliaoxue zazhi |
| spelling | doaj-art-f92a952f4fa049549dfb62980cb71d2d2025-02-08T03:48:35Zzhoeditoiral office of Journal of Diagnosis and Therapy on Dermato-venereologyPifu-xingbing zhenliaoxue zazhi1674-84682025-01-0132161510.3969/j.issn.1674-8468.2025.01.002Study on the role of activation of TGF-β pathway by ASPN in the occurrence and development of keloid and the underlying mechanismsXU Xueyan0CHEN Junyi1YAO Liuyi2DENG Chengcheng3Dermatology Hospital, Southern Medical University, Guangzhou 510091, ChinaDermatology Hospital, Southern Medical University, Guangzhou 510091, ChinaDermatology Hospital, Southern Medical University, Guangzhou 510091, ChinaDermatology Hospital, Southern Medical University, Guangzhou 510091, China[Objective] To investigate the role of ASPN in the development of keloid and the underlying mechanisms. [Methods] Immunofluorescence staining was used to assess the expression levels of ASPN in keloid of keloid patients and normal scar. WB and qRT-PCR were used to assess the activity of TGF-β pathway and expression levels of α-SMA、Collagen Ⅰ/Ⅲ, COMP and POSTN in fibroblasts with overexpression of ASPN, ASPN-treated fibroblasts and Keloid fibroblasts with ASPN knockdown. Moreover, wound healing was observed in C57BL/6J mice treated with or without ASPN at 0 d, 3 d, 7 d, and 14 d after wound. The expression levels of α-SMA、Collagen Ⅰ/Ⅲ and TGF-β in the wounded skin were assessed by immunofluorescence staining 14 days after wound. [Results] ASPN was highly expressed in keloid fibroblasts. The expression levels of α-SMA、Collagen Ⅰ/Ⅲ、COMP and POSTN were significantly increased, and the TGF-β signaling pathway was activated in fibroblasts after either overexpression of or stimulation with ASPN (P<0.05). In contrast, knockdown of ASPN significantly decreased the expression levels of α-SMA、Collagen Ⅰ/Ⅲ, COMP and POSTN and inhibited TGF-β pathway in fibroblasts from keloids (P<0.05). In addition, ASPN significantly increased the expression levels of p-SMAD2、α-SMA and Collagen Ⅰ and activated TGF-β signaling pathway in the wound area (P<0.05 vs. controls), without altering the wound healing process. [Conclusion] ASPN promotes the development of keloid by activating TGF-β signaling pathway.http://pfxbzlx.gdvdc.com/EN/10.3969/j.issn.1674-8468.2025.01.002keloidaspn proteintgf-β pathwaycollagenpostn protein |
| spellingShingle | XU Xueyan CHEN Junyi YAO Liuyi DENG Chengcheng Study on the role of activation of TGF-β pathway by ASPN in the occurrence and development of keloid and the underlying mechanisms Pifu-xingbing zhenliaoxue zazhi keloid aspn protein tgf-β pathway collagen postn protein |
| title | Study on the role of activation of TGF-β pathway by ASPN in the occurrence and development of keloid and the underlying mechanisms |
| title_full | Study on the role of activation of TGF-β pathway by ASPN in the occurrence and development of keloid and the underlying mechanisms |
| title_fullStr | Study on the role of activation of TGF-β pathway by ASPN in the occurrence and development of keloid and the underlying mechanisms |
| title_full_unstemmed | Study on the role of activation of TGF-β pathway by ASPN in the occurrence and development of keloid and the underlying mechanisms |
| title_short | Study on the role of activation of TGF-β pathway by ASPN in the occurrence and development of keloid and the underlying mechanisms |
| title_sort | study on the role of activation of tgf β pathway by aspn in the occurrence and development of keloid and the underlying mechanisms |
| topic | keloid aspn protein tgf-β pathway collagen postn protein |
| url | http://pfxbzlx.gdvdc.com/EN/10.3969/j.issn.1674-8468.2025.01.002 |
| work_keys_str_mv | AT xuxueyan studyontheroleofactivationoftgfbpathwaybyaspnintheoccurrenceanddevelopmentofkeloidandtheunderlyingmechanisms AT chenjunyi studyontheroleofactivationoftgfbpathwaybyaspnintheoccurrenceanddevelopmentofkeloidandtheunderlyingmechanisms AT yaoliuyi studyontheroleofactivationoftgfbpathwaybyaspnintheoccurrenceanddevelopmentofkeloidandtheunderlyingmechanisms AT dengchengcheng studyontheroleofactivationoftgfbpathwaybyaspnintheoccurrenceanddevelopmentofkeloidandtheunderlyingmechanisms |