Study on the role of activation of TGF-β pathway by ASPN in the occurrence and development of keloid and the underlying mechanisms
[Objective] To investigate the role of ASPN in the development of keloid and the underlying mechanisms. [Methods] Immunofluorescence staining was used to assess the expression levels of ASPN in keloid of keloid patients and normal scar. WB and qRT-PCR were used to assess the activity of TGF-β pathwa...
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| Main Authors: | , , , |
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| Format: | Article |
| Language: | zho |
| Published: |
editoiral office of Journal of Diagnosis and Therapy on Dermato-venereology
2025-01-01
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| Series: | Pifu-xingbing zhenliaoxue zazhi |
| Subjects: | |
| Online Access: | http://pfxbzlx.gdvdc.com/EN/10.3969/j.issn.1674-8468.2025.01.002 |
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| Summary: | [Objective] To investigate the role of ASPN in the development of keloid and the underlying mechanisms. [Methods] Immunofluorescence staining was used to assess the expression levels of ASPN in keloid of keloid patients and normal scar. WB and qRT-PCR were used to assess the activity of TGF-β pathway and expression levels of α-SMA、Collagen Ⅰ/Ⅲ, COMP and POSTN in fibroblasts with overexpression of ASPN, ASPN-treated fibroblasts and Keloid fibroblasts with ASPN knockdown. Moreover, wound healing was observed in C57BL/6J mice treated with or without ASPN at 0 d, 3 d, 7 d, and 14 d after wound. The expression levels of α-SMA、Collagen Ⅰ/Ⅲ and TGF-β in the wounded skin were assessed by immunofluorescence staining 14 days after wound. [Results] ASPN was highly expressed in keloid fibroblasts. The expression levels of α-SMA、Collagen Ⅰ/Ⅲ、COMP and POSTN were significantly increased, and the TGF-β signaling pathway was activated in fibroblasts after either overexpression of or stimulation with ASPN (P<0.05). In contrast, knockdown of ASPN significantly decreased the expression levels of α-SMA、Collagen Ⅰ/Ⅲ, COMP and POSTN and inhibited TGF-β pathway in fibroblasts from keloids (P<0.05). In addition, ASPN significantly increased the expression levels of p-SMAD2、α-SMA and Collagen Ⅰ and activated TGF-β signaling pathway in the wound area (P<0.05 vs. controls), without altering the wound healing process. [Conclusion] ASPN promotes the development of keloid by activating TGF-β signaling pathway. |
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| ISSN: | 1674-8468 |