Collagen peptide protects Saccharomyces cerevisiae from furfural stress for enhancing bioethanol synthesis
Abstract The efficient biosynthesis is important for the sustainable development of lignocellulosic ethanol industry, but it is limited by furfural stress produced with cellulose pretreatment. Collagen peptide (CP), as an affluent protein resource, considerably improved the tolerance of Saccharomyce...
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| Format: | Article |
| Language: | English |
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SpringerOpen
2025-02-01
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| Series: | Collagen and Leather |
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| Online Access: | https://doi.org/10.1186/s42825-024-00183-5 |
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| author | Ming Yang Xia Li Bo Wang Xian Liu Bo Zhang Xue-Pin Liao |
| author_facet | Ming Yang Xia Li Bo Wang Xian Liu Bo Zhang Xue-Pin Liao |
| author_sort | Ming Yang |
| collection | DOAJ |
| description | Abstract The efficient biosynthesis is important for the sustainable development of lignocellulosic ethanol industry, but it is limited by furfural stress produced with cellulose pretreatment. Collagen peptide (CP), as an affluent protein resource, considerably improved the tolerance of Saccharomyces cerevisiae against furfural stress. When the furfural concentration was 2 g/L, the residual sugar concentration was reduced from 122.39 to 8.90 g/L, and the final ethanol yield increased from 30.69 to 87.27 g/L in the presence of CP. In addition, the ethanol yield in CP containing media was higher than those in other peptides. Transcriptome analysis showed CP can improve the expression of genes (FBA1, PDC1, PDC6, and ENO1) associated with glycolysis to promote sugar utilization, and enhance ethanol biosynthesis under furfural stress, which were further verified by quantitative real-time PCR. These results indicated that CP is a promising protectant and accelerator for bioethanol biosynthesis. Graphical abstract |
| format | Article |
| id | doaj-art-f921015b6c2c4a29987ec5c00562e575 |
| institution | Kabale University |
| issn | 2097-1419 2731-6998 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | Collagen and Leather |
| spelling | doaj-art-f921015b6c2c4a29987ec5c00562e5752025-02-02T12:42:32ZengSpringerOpenCollagen and Leather2097-14192731-69982025-02-017111410.1186/s42825-024-00183-5Collagen peptide protects Saccharomyces cerevisiae from furfural stress for enhancing bioethanol synthesisMing Yang0Xia Li1Bo Wang2Xian Liu3Bo Zhang4Xue-Pin Liao5College of Biomass Science and Engineering, Sichuan UniversityCollege of Biomass Science and Engineering, Sichuan UniversityCollege of Biomass Science and Engineering, Sichuan UniversityCollege of Biomass Science and Engineering, Sichuan UniversityCollege of Biomass Science and Engineering, Sichuan UniversityCollege of Biomass Science and Engineering, Sichuan UniversityAbstract The efficient biosynthesis is important for the sustainable development of lignocellulosic ethanol industry, but it is limited by furfural stress produced with cellulose pretreatment. Collagen peptide (CP), as an affluent protein resource, considerably improved the tolerance of Saccharomyces cerevisiae against furfural stress. When the furfural concentration was 2 g/L, the residual sugar concentration was reduced from 122.39 to 8.90 g/L, and the final ethanol yield increased from 30.69 to 87.27 g/L in the presence of CP. In addition, the ethanol yield in CP containing media was higher than those in other peptides. Transcriptome analysis showed CP can improve the expression of genes (FBA1, PDC1, PDC6, and ENO1) associated with glycolysis to promote sugar utilization, and enhance ethanol biosynthesis under furfural stress, which were further verified by quantitative real-time PCR. These results indicated that CP is a promising protectant and accelerator for bioethanol biosynthesis. Graphical abstracthttps://doi.org/10.1186/s42825-024-00183-5Collagen peptideLignocellulosic ethanolFermentationFurfuralTranscriptome |
| spellingShingle | Ming Yang Xia Li Bo Wang Xian Liu Bo Zhang Xue-Pin Liao Collagen peptide protects Saccharomyces cerevisiae from furfural stress for enhancing bioethanol synthesis Collagen and Leather Collagen peptide Lignocellulosic ethanol Fermentation Furfural Transcriptome |
| title | Collagen peptide protects Saccharomyces cerevisiae from furfural stress for enhancing bioethanol synthesis |
| title_full | Collagen peptide protects Saccharomyces cerevisiae from furfural stress for enhancing bioethanol synthesis |
| title_fullStr | Collagen peptide protects Saccharomyces cerevisiae from furfural stress for enhancing bioethanol synthesis |
| title_full_unstemmed | Collagen peptide protects Saccharomyces cerevisiae from furfural stress for enhancing bioethanol synthesis |
| title_short | Collagen peptide protects Saccharomyces cerevisiae from furfural stress for enhancing bioethanol synthesis |
| title_sort | collagen peptide protects saccharomyces cerevisiae from furfural stress for enhancing bioethanol synthesis |
| topic | Collagen peptide Lignocellulosic ethanol Fermentation Furfural Transcriptome |
| url | https://doi.org/10.1186/s42825-024-00183-5 |
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