Androgen metabolism and inhibition of interleukin-1 synthesis in primary cultured human synovial macrophages
The presence of androgen receptors on synovial macrophages in human normal and rheumatoid synovial tissues has been described previously. It is now reported that primary cultured human macrophages obtained from normal and rheumatoid synovia express functional androgen receptors. We have investigated...
Saved in:
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
1995-01-01
|
| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/S096293519500024X |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832560916087963648 |
|---|---|
| author | M. Cutolo S. Accardo B. Villaggio A. Barone A. Sulli E. Balleari C. Bason L. Felli O. M. Granata R. Amodio L. Castagnetta |
| author_facet | M. Cutolo S. Accardo B. Villaggio A. Barone A. Sulli E. Balleari C. Bason L. Felli O. M. Granata R. Amodio L. Castagnetta |
| author_sort | M. Cutolo |
| collection | DOAJ |
| description | The presence of androgen receptors on synovial macrophages in human normal and rheumatoid synovial tissues has been described previously. It is now reported that primary cultured human macrophages obtained from normal and rheumatoid synovia express functional androgen receptors. We have investigated the capacity of cultured macrophages to metabolize androgens and have found that these cells were capable of metabolizing testosterone to the bioactive metabolite dihydrotestosterone. Therefore, macrophages contain the key enzymes of steroidogenesis, in particular the 5α-treductase. Furthermore, interleukin-1β production by primary cultured rheumatoid macrophages was analysed, following exposure to physiological concentrations of testosterone (10−8 M). A significant decrease of IL-1β levels in conditioned media after 24 h (p < 0.05) was observed. It is concluded that androgens may act directly on human macrophages and may interfere with some of their functions via receptor-dependent mechanisms. |
| format | Article |
| id | doaj-art-f91e070dd0094fa7a17468f6f4ebc80b |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 1995-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-f91e070dd0094fa7a17468f6f4ebc80b2025-02-03T01:26:31ZengWileyMediators of Inflammation0962-93511466-18611995-01-014213814310.1155/S096293519500024XAndrogen metabolism and inhibition of interleukin-1 synthesis in primary cultured human synovial macrophagesM. Cutolo0S. Accardo1B. Villaggio2A. Barone3A. Sulli4E. Balleari5C. Bason6L. Felli7O. M. Granata8R. Amodio9L. Castagnetta10Division of Rheumatology, University of Genova, Viale Benedetto XV, Genova 16132, ItalyDivision of Rheumatology, University of Genova, Viale Benedetto XV, Genova 16132, ItalyDivision of Nephrology, University of Genova, Viale Benedetto XV, Genova 16132, ItalyDivision of Rheumatology, University of Genova, Viale Benedetto XV, Genova 16132, ItalyDivision of Rheumatology, University of Genova, Viale Benedetto XV, Genova 16132, ItalyDepartment of Internal Medicine, University of Genova, Viale Benedetto XV, Genova 16132, ItalyDepartment of Internal Medicine, University of Genova, Viale Benedetto XV, Genova 16132, ItalyDivision of Orthopedic Surgery, University of Genova, Viale Benedetto XV, Genova 16132, ItalyHormone Biochemistry Laboratories, University of Palermo, ItalyDivision of Orthopedic Surgery, University of Genova, Viale Benedetto XV, Genova 16132, ItalyHormone Biochemistry Laboratories, University of Palermo, ItalyThe presence of androgen receptors on synovial macrophages in human normal and rheumatoid synovial tissues has been described previously. It is now reported that primary cultured human macrophages obtained from normal and rheumatoid synovia express functional androgen receptors. We have investigated the capacity of cultured macrophages to metabolize androgens and have found that these cells were capable of metabolizing testosterone to the bioactive metabolite dihydrotestosterone. Therefore, macrophages contain the key enzymes of steroidogenesis, in particular the 5α-treductase. Furthermore, interleukin-1β production by primary cultured rheumatoid macrophages was analysed, following exposure to physiological concentrations of testosterone (10−8 M). A significant decrease of IL-1β levels in conditioned media after 24 h (p < 0.05) was observed. It is concluded that androgens may act directly on human macrophages and may interfere with some of their functions via receptor-dependent mechanisms.http://dx.doi.org/10.1155/S096293519500024X |
| spellingShingle | M. Cutolo S. Accardo B. Villaggio A. Barone A. Sulli E. Balleari C. Bason L. Felli O. M. Granata R. Amodio L. Castagnetta Androgen metabolism and inhibition of interleukin-1 synthesis in primary cultured human synovial macrophages Mediators of Inflammation |
| title | Androgen metabolism and inhibition of interleukin-1 synthesis in primary cultured human synovial macrophages |
| title_full | Androgen metabolism and inhibition of interleukin-1 synthesis in primary cultured human synovial macrophages |
| title_fullStr | Androgen metabolism and inhibition of interleukin-1 synthesis in primary cultured human synovial macrophages |
| title_full_unstemmed | Androgen metabolism and inhibition of interleukin-1 synthesis in primary cultured human synovial macrophages |
| title_short | Androgen metabolism and inhibition of interleukin-1 synthesis in primary cultured human synovial macrophages |
| title_sort | androgen metabolism and inhibition of interleukin 1 synthesis in primary cultured human synovial macrophages |
| url | http://dx.doi.org/10.1155/S096293519500024X |
| work_keys_str_mv | AT mcutolo androgenmetabolismandinhibitionofinterleukin1synthesisinprimaryculturedhumansynovialmacrophages AT saccardo androgenmetabolismandinhibitionofinterleukin1synthesisinprimaryculturedhumansynovialmacrophages AT bvillaggio androgenmetabolismandinhibitionofinterleukin1synthesisinprimaryculturedhumansynovialmacrophages AT abarone androgenmetabolismandinhibitionofinterleukin1synthesisinprimaryculturedhumansynovialmacrophages AT asulli androgenmetabolismandinhibitionofinterleukin1synthesisinprimaryculturedhumansynovialmacrophages AT eballeari androgenmetabolismandinhibitionofinterleukin1synthesisinprimaryculturedhumansynovialmacrophages AT cbason androgenmetabolismandinhibitionofinterleukin1synthesisinprimaryculturedhumansynovialmacrophages AT lfelli androgenmetabolismandinhibitionofinterleukin1synthesisinprimaryculturedhumansynovialmacrophages AT omgranata androgenmetabolismandinhibitionofinterleukin1synthesisinprimaryculturedhumansynovialmacrophages AT ramodio androgenmetabolismandinhibitionofinterleukin1synthesisinprimaryculturedhumansynovialmacrophages AT lcastagnetta androgenmetabolismandinhibitionofinterleukin1synthesisinprimaryculturedhumansynovialmacrophages |