Preimplantation aneuploidy screening using embryonic cell-free DNA isolated from spent culture medium: a case report
Abstract Background Chromosomal aberrations are frequent in human embryos and can be identified during IVF by performing preimplantation genetic testing for aneuploidies (PGT-A). Therefore, this study presents a case report highlighting the application of embryonic cell-free DNA isolated from spent...
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| Format: | Article |
| Language: | English |
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SpringerOpen
2025-02-01
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| Series: | Egyptian Journal of Medical Human Genetics |
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| Online Access: | https://doi.org/10.1186/s43042-025-00654-2 |
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| author | Lama Khudari Marwan Halabi Sahar Al Fahoum |
| author_facet | Lama Khudari Marwan Halabi Sahar Al Fahoum |
| author_sort | Lama Khudari |
| collection | DOAJ |
| description | Abstract Background Chromosomal aberrations are frequent in human embryos and can be identified during IVF by performing preimplantation genetic testing for aneuploidies (PGT-A). Therefore, this study presents a case report highlighting the application of embryonic cell-free DNA isolated from spent culture medium for preimplantation aneuploidy screening. Case presentation This case report elucidates the potential use of non-invasive preimplantation genetic testing for aneuploidy (PGT-A) as a valuable resource for couples facing infertility challenges. By using PGT-A, these couples can assess the genetic health of embryos before implantation, thereby increasing the chances of a successful pregnancy through assisted reproductive technologies. Conclusions PGT-A, an innovative technique using embryonic cell-free DNA isolated from a spent culture medium, offers a non-invasive and effective method for assessing chromosomal aberrations in embryos. It has the potential to enhance preimplantation genetic testing and support medically personalized embryo selection. |
| format | Article |
| id | doaj-art-f910803416624b009b37ce043fb76604 |
| institution | Kabale University |
| issn | 2090-2441 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | Egyptian Journal of Medical Human Genetics |
| spelling | doaj-art-f910803416624b009b37ce043fb766042025-02-09T12:40:11ZengSpringerOpenEgyptian Journal of Medical Human Genetics2090-24412025-02-012611410.1186/s43042-025-00654-2Preimplantation aneuploidy screening using embryonic cell-free DNA isolated from spent culture medium: a case reportLama Khudari0Marwan Halabi1Sahar Al Fahoum2Department of Biochemistry and Microbiology, Faculty of Pharmacy, Damascus UniversityDepartment of Anatomy, Histology and Embryology, Faculty of Medicine, Damascus UniversityDepartment of Biochemistry and Microbiology, Faculty of Pharmacy, Damascus UniversityAbstract Background Chromosomal aberrations are frequent in human embryos and can be identified during IVF by performing preimplantation genetic testing for aneuploidies (PGT-A). Therefore, this study presents a case report highlighting the application of embryonic cell-free DNA isolated from spent culture medium for preimplantation aneuploidy screening. Case presentation This case report elucidates the potential use of non-invasive preimplantation genetic testing for aneuploidy (PGT-A) as a valuable resource for couples facing infertility challenges. By using PGT-A, these couples can assess the genetic health of embryos before implantation, thereby increasing the chances of a successful pregnancy through assisted reproductive technologies. Conclusions PGT-A, an innovative technique using embryonic cell-free DNA isolated from a spent culture medium, offers a non-invasive and effective method for assessing chromosomal aberrations in embryos. It has the potential to enhance preimplantation genetic testing and support medically personalized embryo selection.https://doi.org/10.1186/s43042-025-00654-2Embryonic cell-free DNAPreimplantation genetic testing (PGT-A)AneuploidySpent culture mediumCase report |
| spellingShingle | Lama Khudari Marwan Halabi Sahar Al Fahoum Preimplantation aneuploidy screening using embryonic cell-free DNA isolated from spent culture medium: a case report Egyptian Journal of Medical Human Genetics Embryonic cell-free DNA Preimplantation genetic testing (PGT-A) Aneuploidy Spent culture medium Case report |
| title | Preimplantation aneuploidy screening using embryonic cell-free DNA isolated from spent culture medium: a case report |
| title_full | Preimplantation aneuploidy screening using embryonic cell-free DNA isolated from spent culture medium: a case report |
| title_fullStr | Preimplantation aneuploidy screening using embryonic cell-free DNA isolated from spent culture medium: a case report |
| title_full_unstemmed | Preimplantation aneuploidy screening using embryonic cell-free DNA isolated from spent culture medium: a case report |
| title_short | Preimplantation aneuploidy screening using embryonic cell-free DNA isolated from spent culture medium: a case report |
| title_sort | preimplantation aneuploidy screening using embryonic cell free dna isolated from spent culture medium a case report |
| topic | Embryonic cell-free DNA Preimplantation genetic testing (PGT-A) Aneuploidy Spent culture medium Case report |
| url | https://doi.org/10.1186/s43042-025-00654-2 |
| work_keys_str_mv | AT lamakhudari preimplantationaneuploidyscreeningusingembryoniccellfreednaisolatedfromspentculturemediumacasereport AT marwanhalabi preimplantationaneuploidyscreeningusingembryoniccellfreednaisolatedfromspentculturemediumacasereport AT saharalfahoum preimplantationaneuploidyscreeningusingembryoniccellfreednaisolatedfromspentculturemediumacasereport |