Cryptosporidium spp. prevalence in the general population in Guinea: first large-scale screening study
Cryptosporidium is a leading cause of diarrheal mortality in children in Africa and Asia. Despite the public health significance of this parasite, its molecular epidemiology and circulation in Guinea remain poorly understood. Therefore, this study aimed to determine the prevalence and genotype distr...
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EDP Sciences
2024-01-01
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| author | Guilavogui Timothé Gantois Nausicaa Desramaut Jérémy Cissé Fode Ibrahima Touré Salif Cherif Kourouma Bakary Luther Preda Cristian Chabé Magali Viscogliosi Eric Certad Gabriela |
| author_facet | Guilavogui Timothé Gantois Nausicaa Desramaut Jérémy Cissé Fode Ibrahima Touré Salif Cherif Kourouma Bakary Luther Preda Cristian Chabé Magali Viscogliosi Eric Certad Gabriela |
| author_sort | Guilavogui Timothé |
| collection | DOAJ |
| description | Cryptosporidium is a leading cause of diarrheal mortality in children in Africa and Asia. Despite the public health significance of this parasite, its molecular epidemiology and circulation in Guinea remain poorly understood. Therefore, this study aimed to determine the prevalence and genotype distribution of Cryptosporidium in the Guinean general population. To achieve this, fecal samples were collected from 834 individuals, both with and without digestive disorders, at two hospitals in Conakry. The presence of the parasite in the stool samples was detected using nested PCR targeting the SSU rDNA gene, followed by sequencing of the PCR products for genotyping of the isolates. The PCR-based prevalence was 0.12% for the whole cohort, and 0.2% among adults. The low frequency of Cryptosporidium observed in the current study is thus consistent with the prevalence of this parasite already reported in certain other African countries. The species identified in the positive samples was Cryptosporidium hominis. This study is the first to report the prevalence of Cryptosporidium in the general population of Guinea. Given the potential of this parasite to cause life-threatening diarrhea, further studies are needed to clarify the epidemiology of Cryptosporidium in this country. |
| format | Article |
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| institution | OA Journals |
| issn | 1776-1042 |
| language | English |
| publishDate | 2024-01-01 |
| publisher | EDP Sciences |
| record_format | Article |
| series | Parasite |
| spelling | doaj-art-f90febdb491a446b972becfd17fbdb1a2025-08-20T02:31:08ZengEDP SciencesParasite1776-10422024-01-01317010.1051/parasite/2024070parasite240145Cryptosporidium spp. prevalence in the general population in Guinea: first large-scale screening studyGuilavogui Timothé0Gantois Nausicaa1Desramaut Jérémy2Cissé Fode Ibrahima3Touré Salif Cherif4Kourouma Bakary Luther5Preda Cristian6Chabé Magali7Viscogliosi Eric8Certad Gabriela9CNRS, Inserm, CHU Lille, Institut Pasteur de Lille, U1019-UMR 9017-CIIL-Centre d’Infection et d’Immunité de Lille, Université de LilleCNRS, Inserm, CHU Lille, Institut Pasteur de Lille, U1019-UMR 9017-CIIL-Centre d’Infection et d’Immunité de Lille, Université de LilleCNRS, Inserm, CHU Lille, Institut Pasteur de Lille, U1019-UMR 9017-CIIL-Centre d’Infection et d’Immunité de Lille, Université de LilleHôpital National Ignace Deen CHU de Conakry, Laboratoire de ParasitologieHôpital National Ignace Deen CHU de Conakry, Laboratoire de ParasitologieCentre de Santé AnastasisDélégation à la Recherche Clinique et à l’Innovation, Groupement des Hôpitaux de l’Institut Catholique de Lille, Lille Catholic UniversityCNRS, Inserm, CHU Lille, Institut Pasteur de Lille, U1019-UMR 9017-CIIL-Centre d’Infection et d’Immunité de Lille, Université de LilleCNRS, Inserm, CHU Lille, Institut Pasteur de Lille, U1019-UMR 9017-CIIL-Centre d’Infection et d’Immunité de Lille, Université de LilleCNRS, Inserm, CHU Lille, Institut Pasteur de Lille, U1019-UMR 9017-CIIL-Centre d’Infection et d’Immunité de Lille, Université de LilleCryptosporidium is a leading cause of diarrheal mortality in children in Africa and Asia. Despite the public health significance of this parasite, its molecular epidemiology and circulation in Guinea remain poorly understood. Therefore, this study aimed to determine the prevalence and genotype distribution of Cryptosporidium in the Guinean general population. To achieve this, fecal samples were collected from 834 individuals, both with and without digestive disorders, at two hospitals in Conakry. The presence of the parasite in the stool samples was detected using nested PCR targeting the SSU rDNA gene, followed by sequencing of the PCR products for genotyping of the isolates. The PCR-based prevalence was 0.12% for the whole cohort, and 0.2% among adults. The low frequency of Cryptosporidium observed in the current study is thus consistent with the prevalence of this parasite already reported in certain other African countries. The species identified in the positive samples was Cryptosporidium hominis. This study is the first to report the prevalence of Cryptosporidium in the general population of Guinea. Given the potential of this parasite to cause life-threatening diarrhea, further studies are needed to clarify the epidemiology of Cryptosporidium in this country.https://www.parasite-journal.org/articles/parasite/full_html/2024/01/parasite240145/parasite240145.htmlcryptosporidium sppmolecular epidemiologytransmissionzoonosisguineaafrica |
| spellingShingle | Guilavogui Timothé Gantois Nausicaa Desramaut Jérémy Cissé Fode Ibrahima Touré Salif Cherif Kourouma Bakary Luther Preda Cristian Chabé Magali Viscogliosi Eric Certad Gabriela Cryptosporidium spp. prevalence in the general population in Guinea: first large-scale screening study Parasite cryptosporidium spp molecular epidemiology transmission zoonosis guinea africa |
| title | Cryptosporidium spp. prevalence in the general population in Guinea: first large-scale screening study |
| title_full | Cryptosporidium spp. prevalence in the general population in Guinea: first large-scale screening study |
| title_fullStr | Cryptosporidium spp. prevalence in the general population in Guinea: first large-scale screening study |
| title_full_unstemmed | Cryptosporidium spp. prevalence in the general population in Guinea: first large-scale screening study |
| title_short | Cryptosporidium spp. prevalence in the general population in Guinea: first large-scale screening study |
| title_sort | cryptosporidium spp prevalence in the general population in guinea first large scale screening study |
| topic | cryptosporidium spp molecular epidemiology transmission zoonosis guinea africa |
| url | https://www.parasite-journal.org/articles/parasite/full_html/2024/01/parasite240145/parasite240145.html |
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