Novel ellipsoid-like granules exhibit enhanced anammox performance compared to sphere-like granules

Anammox granular sludge (AnGS) serves as an important platform for cost-effective nitrogen removal from wastewater. Different to the traditionally sphere-like granules, a novel type of AnGS in a unique ellipsoid-like shape was obtained through enhancing shear force. The ellipsoid-like AnGS significa...

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Main Authors: Dongdong Xu, Tao Liu, Chao Pan, Leiyan Guo, Jianhua Guo, Ping Zheng, Meng Zhang
Format: Article
Language:English
Published: Elsevier 2024-12-01
Series:Water Research X
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Online Access:http://www.sciencedirect.com/science/article/pii/S2589914724000604
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author Dongdong Xu
Tao Liu
Chao Pan
Leiyan Guo
Jianhua Guo
Ping Zheng
Meng Zhang
author_facet Dongdong Xu
Tao Liu
Chao Pan
Leiyan Guo
Jianhua Guo
Ping Zheng
Meng Zhang
author_sort Dongdong Xu
collection DOAJ
description Anammox granular sludge (AnGS) serves as an important platform for cost-effective nitrogen removal from wastewater. Different to the traditionally sphere-like granules, a novel type of AnGS in a unique ellipsoid-like shape was obtained through enhancing shear force. The ellipsoid-like AnGS significantly exhibited a smaller aspect ratio (-25.1 %) and granular size (-11.8 %), compared to traditional sphere-like AnGS (p < 0.01). Comprehensive comparisons showed that ellipsoid-like AnGS possessed a significantly higher extracellular polymeric substances (EPS) content and strength, as well as an enhanced mass transfer and a higher viable bacteria proportion due to the larger substrate permeable zone (p < 0.01). Additionally, the anammox bacterial abundance (Candidatus Kuenenia) was 12.2 % higher in ellipsoid-like AnGS than in sphere-like AnGS. All these characteristics of ellipsoid-like AnGS jointly increased the specific anammox activity by 29.0 % and nitrogen removal capacity by 22.6 %, compared to sphere-like AnGS. Further fluid field simulation suggested the enhanced flow shear on the side surface of AnGS likely drove the formation of ellipsoid-like AnGS. The higher shear force on the side surface led to an increase of EPS content (especially hydrophobic protein) and elastic modulus, thus constraining lateral expansion. This study sheds light on impacts of granular shape, an overlooked morphological factor, on anammox performance. The ellipsoid-like AnGS presented herein also offers a unique and promising aggregate to enhance anammox performance.
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spelling doaj-art-f90f2f2239d94a1b8551f906e4c406572024-11-22T07:38:44ZengElsevierWater Research X2589-91472024-12-0125100270Novel ellipsoid-like granules exhibit enhanced anammox performance compared to sphere-like granulesDongdong Xu0Tao Liu1Chao Pan2Leiyan Guo3Jianhua Guo4Ping Zheng5Meng Zhang6Department of Environmental Engineering, College of Environmental &amp; Resource Sciences, Zhejiang University, Hangzhou, China; Australian Centre for Water and Environmental Biotechnology (ACWEB, formerly AWMC), The University of Queensland, St. Lucia, Queensland 4072, AustraliaAustralian Centre for Water and Environmental Biotechnology (ACWEB, formerly AWMC), The University of Queensland, St. Lucia, Queensland 4072, AustraliaDepartment of Environmental Engineering, College of Environmental &amp; Resource Sciences, Zhejiang University, Hangzhou, ChinaDepartment of Environmental Engineering, College of Environmental &amp; Resource Sciences, Zhejiang University, Hangzhou, ChinaAustralian Centre for Water and Environmental Biotechnology (ACWEB, formerly AWMC), The University of Queensland, St. Lucia, Queensland 4072, Australia; Corresponding authors.Department of Environmental Engineering, College of Environmental &amp; Resource Sciences, Zhejiang University, Hangzhou, China; Zhejiang Province Key Laboratory for Water Pollution Control and Environmental Safety, Hangzhou, ChinaDepartment of Environmental Engineering, College of Environmental &amp; Resource Sciences, Zhejiang University, Hangzhou, China; Zhejiang Province Key Laboratory for Water Pollution Control and Environmental Safety, Hangzhou, China; Innovation Center of Yangtze River Delta, Zhejiang University, Jiashan 314100, China; Corresponding authors.Anammox granular sludge (AnGS) serves as an important platform for cost-effective nitrogen removal from wastewater. Different to the traditionally sphere-like granules, a novel type of AnGS in a unique ellipsoid-like shape was obtained through enhancing shear force. The ellipsoid-like AnGS significantly exhibited a smaller aspect ratio (-25.1 %) and granular size (-11.8 %), compared to traditional sphere-like AnGS (p < 0.01). Comprehensive comparisons showed that ellipsoid-like AnGS possessed a significantly higher extracellular polymeric substances (EPS) content and strength, as well as an enhanced mass transfer and a higher viable bacteria proportion due to the larger substrate permeable zone (p < 0.01). Additionally, the anammox bacterial abundance (Candidatus Kuenenia) was 12.2 % higher in ellipsoid-like AnGS than in sphere-like AnGS. All these characteristics of ellipsoid-like AnGS jointly increased the specific anammox activity by 29.0 % and nitrogen removal capacity by 22.6 %, compared to sphere-like AnGS. Further fluid field simulation suggested the enhanced flow shear on the side surface of AnGS likely drove the formation of ellipsoid-like AnGS. The higher shear force on the side surface led to an increase of EPS content (especially hydrophobic protein) and elastic modulus, thus constraining lateral expansion. This study sheds light on impacts of granular shape, an overlooked morphological factor, on anammox performance. The ellipsoid-like AnGS presented herein also offers a unique and promising aggregate to enhance anammox performance.http://www.sciencedirect.com/science/article/pii/S2589914724000604Anammox granular sludgeFlow shearGranular morphologyShapeMass transferAnammox activity
spellingShingle Dongdong Xu
Tao Liu
Chao Pan
Leiyan Guo
Jianhua Guo
Ping Zheng
Meng Zhang
Novel ellipsoid-like granules exhibit enhanced anammox performance compared to sphere-like granules
Water Research X
Anammox granular sludge
Flow shear
Granular morphology
Shape
Mass transfer
Anammox activity
title Novel ellipsoid-like granules exhibit enhanced anammox performance compared to sphere-like granules
title_full Novel ellipsoid-like granules exhibit enhanced anammox performance compared to sphere-like granules
title_fullStr Novel ellipsoid-like granules exhibit enhanced anammox performance compared to sphere-like granules
title_full_unstemmed Novel ellipsoid-like granules exhibit enhanced anammox performance compared to sphere-like granules
title_short Novel ellipsoid-like granules exhibit enhanced anammox performance compared to sphere-like granules
title_sort novel ellipsoid like granules exhibit enhanced anammox performance compared to sphere like granules
topic Anammox granular sludge
Flow shear
Granular morphology
Shape
Mass transfer
Anammox activity
url http://www.sciencedirect.com/science/article/pii/S2589914724000604
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