Metagenome Analysis Reveals Changes in Gut Microbial Antibiotic Resistance Genes and Virulence Factors in Reintroduced Giant Pandas

Metagenome Analysis Reveals Changes in Gut Microbial Antibiotic Resistance Genes and Virulence Factors in Reintroduced Giant Pandas

Antibiotic resistance has emerged as a critical global public health challenge. In this study, we employed metagenomic sequencing to analyze fecal samples from giant pandas (<i>Ailuropoda melanoleuca</i>) across three distinct stages—semi-wild, released, and wild populations—to investiga...

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Bibliographic Details
Main Authors: Wanju Feng, Chenyi Gao, Xinyuan Cui, Bing Yang, Ke He, Qiuyu Huang, Xinru Yang, Kaizhi Wen, Jiadong Xie, Zhisong Yang, Lifeng Zhu
Format: Article
Language:English
Published: MDPI AG 2025-07-01
Series:Microorganisms
Subjects:
giant pandas
metagenome
antibiotic resistance gene (ARG)
virulence factor (VF)
Online Access:https://www.mdpi.com/2076-2607/13/7/1616
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Summary:Antibiotic resistance has emerged as a critical global public health challenge. In this study, we employed metagenomic sequencing to analyze fecal samples from giant pandas (<i>Ailuropoda melanoleuca</i>) across three distinct stages—semi-wild, released, and wild populations—to investigate shifts in antibiotic resistance genes (ARGs) and virulence factors (VFs) during the reintroduction process. Our findings revealed significant variations in the composition of ARG and VF across different stages, with released and wild giant pandas exhibiting similar ARG and VF profiles. Further analyses identified that the increased abundance of ARGs and VFs in both released and wild individuals compared to semi-wild individuals was mainly from <i>Pseudomonas</i>. We hypothesized that the same geographic environment in which ARGs and VFs are transmitted between a host and the environment via mobile genetic elements (MGEs) may be responsible for the similar structure of ARGs and VFs in released and wild giant pandas. Additionally, diet may modulate the gut microbial community, thereby influencing the distributions of ARG and VF. This study elucidated the impact of geographic and dietary factors on ARGs and VFs dynamics in giant pandas, offering valuable insights for mitigating antibiotic resistance and virulence gene dissemination.
ISSN:2076-2607

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