Variations in the milk lipidomic profile of lactating dairy cows fed the diets containing alfalfa hay versus alfalfa silage

Alfalfa is primarily stored as silage or hay in livestock production. Previous research has shown that the storage method of grass significantly influences milk composition. This study aimed to investigate milk production performance and lipid composition in dairy cows fed diets consisting of alfalf...

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Main Authors: Kaizhen Liu, Meiqing Chen, Guoxin Huang, Chuanyou Su, Wenhao Tang, Ning Li, Jiyong Yang, Xufang Wu, Boxue Si, Shengguo Zhao, Nan Zheng, Yangdong Zhang, Jiaqi Wang
Format: Article
Language:English
Published: KeAi Communications Co., Ltd. 2024-12-01
Series:Animal Nutrition
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Online Access:http://www.sciencedirect.com/science/article/pii/S2405654524001379
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author Kaizhen Liu
Meiqing Chen
Guoxin Huang
Chuanyou Su
Wenhao Tang
Ning Li
Jiyong Yang
Xufang Wu
Boxue Si
Shengguo Zhao
Nan Zheng
Yangdong Zhang
Jiaqi Wang
author_facet Kaizhen Liu
Meiqing Chen
Guoxin Huang
Chuanyou Su
Wenhao Tang
Ning Li
Jiyong Yang
Xufang Wu
Boxue Si
Shengguo Zhao
Nan Zheng
Yangdong Zhang
Jiaqi Wang
author_sort Kaizhen Liu
collection DOAJ
description Alfalfa is primarily stored as silage or hay in livestock production. Previous research has shown that the storage method of grass significantly influences milk composition. This study aimed to investigate milk production performance and lipid composition in dairy cows fed diets consisting of alfalfa hay or alfalfa silage as roughage. Forty-two mid-lactation Holstein dairy cows were selected and randomly divided into three groups, each receiving a total mixed ration consisting of alfalfa hay (AH), 50% alfalfa silage + 50% alfalfa hay (AHAS), or alfalfa silage (AS). The results showed that milk fat content (P = 0.049) and milk fat yield (P < 0.001) were significantly higher in the AH and AHAS groups compared to the AH group. With increased supplementation of alfalfa silage in the diet, ω-3 polyunsaturated fatty acid content increased significantly (P < 0.001), while ω-6 polyunsaturated fatty acid content (P = 0.007) and the ratio of ω-6 to ω-3 polyunsaturated fatty acids decreased (P < 0.001). The contents of sphingomyelins, phosphatidylserines, phosphatidylethanolamines, and phosphatidylglycerols in the AHAS and AS samples were higher than in the AH samples, although the differences were not statistically significant. Additionally, the content of phosphatidylcholines was significantly higher in the AS group compared to the AH group (P = 0.032). In conclusion, feeding dairy cows a diet consisting of alfalfa silage can increase the major phospholipid content and polyunsaturated fatty acid composition in raw milk, which is more conducive to human health. These findings provide valuable insights into the benefits of alfalfa silage for dairy cows.
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spelling doaj-art-f8e4ca0a767a4fc8afff2c19082476c52025-08-20T02:51:19ZengKeAi Communications Co., Ltd.Animal Nutrition2405-65452024-12-011926127110.1016/j.aninu.2024.06.009Variations in the milk lipidomic profile of lactating dairy cows fed the diets containing alfalfa hay versus alfalfa silageKaizhen Liu0Meiqing Chen1Guoxin Huang2Chuanyou Su3Wenhao Tang4Ning Li5Jiyong Yang6Xufang Wu7Boxue Si8Shengguo Zhao9Nan Zheng10Yangdong Zhang11Jiaqi Wang12Key Laboratory of Quality &amp; Safety Control for Milk and Dairy Products of Ministry of Agriculture and Rural Affairs, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China; Henan International Joint Laboratory of Nutrition Regulation and Ecological Raising of Domestic Animal, College of Animal Science and Technology, Henan Agricultural University, Zhengzhou 450046, China; State Key Laboratory of Animal Nutrition, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, ChinaKey Laboratory of Quality &amp; Safety Control for Milk and Dairy Products of Ministry of Agriculture and Rural Affairs, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China; State Key Laboratory of Animal Nutrition, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, ChinaKey Laboratory of Quality &amp; Safety Control for Milk and Dairy Products of Ministry of Agriculture and Rural Affairs, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China; State Key Laboratory of Animal Nutrition, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, ChinaKey Laboratory of Quality &amp; Safety Control for Milk and Dairy Products of Ministry of Agriculture and Rural Affairs, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China; Henan International Joint Laboratory of Nutrition Regulation and Ecological Raising of Domestic Animal, College of Animal Science and Technology, Henan Agricultural University, Zhengzhou 450046, China; State Key Laboratory of Animal Nutrition, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, ChinaKey Laboratory of Quality &amp; Safety Control for Milk and Dairy Products of Ministry of Agriculture and Rural Affairs, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China; State Key Laboratory of Animal Nutrition, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, ChinaKey Laboratory of Quality &amp; Safety Control for Milk and Dairy Products of Ministry of Agriculture and Rural Affairs, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China; State Key Laboratory of Animal Nutrition, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, ChinaKey Laboratory of Quality &amp; Safety Control for Milk and Dairy Products of Ministry of Agriculture and Rural Affairs, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China; State Key Laboratory of Animal Nutrition, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, ChinaKey Laboratory of Quality &amp; Safety Control for Milk and Dairy Products of Ministry of Agriculture and Rural Affairs, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China; State Key Laboratory of Animal Nutrition, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, ChinaKey Laboratory of Quality &amp; Safety Control for Milk and Dairy Products of Ministry of Agriculture and Rural Affairs, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China; State Key Laboratory of Animal Nutrition, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, ChinaKey Laboratory of Quality &amp; Safety Control for Milk and Dairy Products of Ministry of Agriculture and Rural Affairs, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China; State Key Laboratory of Animal Nutrition, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, ChinaKey Laboratory of Quality &amp; Safety Control for Milk and Dairy Products of Ministry of Agriculture and Rural Affairs, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China; State Key Laboratory of Animal Nutrition, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, ChinaKey Laboratory of Quality &amp; Safety Control for Milk and Dairy Products of Ministry of Agriculture and Rural Affairs, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China; State Key Laboratory of Animal Nutrition, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China; Corresponding author.Key Laboratory of Quality &amp; Safety Control for Milk and Dairy Products of Ministry of Agriculture and Rural Affairs, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China; State Key Laboratory of Animal Nutrition, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China; Corresponding author.Alfalfa is primarily stored as silage or hay in livestock production. Previous research has shown that the storage method of grass significantly influences milk composition. This study aimed to investigate milk production performance and lipid composition in dairy cows fed diets consisting of alfalfa hay or alfalfa silage as roughage. Forty-two mid-lactation Holstein dairy cows were selected and randomly divided into three groups, each receiving a total mixed ration consisting of alfalfa hay (AH), 50% alfalfa silage + 50% alfalfa hay (AHAS), or alfalfa silage (AS). The results showed that milk fat content (P = 0.049) and milk fat yield (P < 0.001) were significantly higher in the AH and AHAS groups compared to the AH group. With increased supplementation of alfalfa silage in the diet, ω-3 polyunsaturated fatty acid content increased significantly (P < 0.001), while ω-6 polyunsaturated fatty acid content (P = 0.007) and the ratio of ω-6 to ω-3 polyunsaturated fatty acids decreased (P < 0.001). The contents of sphingomyelins, phosphatidylserines, phosphatidylethanolamines, and phosphatidylglycerols in the AHAS and AS samples were higher than in the AH samples, although the differences were not statistically significant. Additionally, the content of phosphatidylcholines was significantly higher in the AS group compared to the AH group (P = 0.032). In conclusion, feeding dairy cows a diet consisting of alfalfa silage can increase the major phospholipid content and polyunsaturated fatty acid composition in raw milk, which is more conducive to human health. These findings provide valuable insights into the benefits of alfalfa silage for dairy cows.http://www.sciencedirect.com/science/article/pii/S2405654524001379Dairy cowAlfalfa silageAlfalfa hayMilk fatLipidomic profileFatty acid
spellingShingle Kaizhen Liu
Meiqing Chen
Guoxin Huang
Chuanyou Su
Wenhao Tang
Ning Li
Jiyong Yang
Xufang Wu
Boxue Si
Shengguo Zhao
Nan Zheng
Yangdong Zhang
Jiaqi Wang
Variations in the milk lipidomic profile of lactating dairy cows fed the diets containing alfalfa hay versus alfalfa silage
Animal Nutrition
Dairy cow
Alfalfa silage
Alfalfa hay
Milk fat
Lipidomic profile
Fatty acid
title Variations in the milk lipidomic profile of lactating dairy cows fed the diets containing alfalfa hay versus alfalfa silage
title_full Variations in the milk lipidomic profile of lactating dairy cows fed the diets containing alfalfa hay versus alfalfa silage
title_fullStr Variations in the milk lipidomic profile of lactating dairy cows fed the diets containing alfalfa hay versus alfalfa silage
title_full_unstemmed Variations in the milk lipidomic profile of lactating dairy cows fed the diets containing alfalfa hay versus alfalfa silage
title_short Variations in the milk lipidomic profile of lactating dairy cows fed the diets containing alfalfa hay versus alfalfa silage
title_sort variations in the milk lipidomic profile of lactating dairy cows fed the diets containing alfalfa hay versus alfalfa silage
topic Dairy cow
Alfalfa silage
Alfalfa hay
Milk fat
Lipidomic profile
Fatty acid
url http://www.sciencedirect.com/science/article/pii/S2405654524001379
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