Epigallocatechin-3-gallate Protects against Hydrogen Peroxide-Induced Inhibition of Osteogenic Differentiation of Human Bone Marrow-Derived Mesenchymal Stem Cells
Oxidative stress induces bone loss and osteoporosis, and epigallocatechin-3-gallate (EGCG) may be used to combat these diseases due to its antioxidative property. Herein, oxidative stress in human bone marrow-derived mesenchymal stem cells (BM-MSCs) was induced by H2O2, resulting in an adverse effec...
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| Format: | Article |
| Language: | English |
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Wiley
2016-01-01
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| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2016/7532798 |
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| author | Dawei Wang Yonghui Wang Shihong Xu Fu Wang Bomin Wang Ke Han Daqing Sun Lianxin Li |
| author_facet | Dawei Wang Yonghui Wang Shihong Xu Fu Wang Bomin Wang Ke Han Daqing Sun Lianxin Li |
| author_sort | Dawei Wang |
| collection | DOAJ |
| description | Oxidative stress induces bone loss and osteoporosis, and epigallocatechin-3-gallate (EGCG) may be used to combat these diseases due to its antioxidative property. Herein, oxidative stress in human bone marrow-derived mesenchymal stem cells (BM-MSCs) was induced by H2O2, resulting in an adverse effect on their osteogenic differentiation. However, this H2O2-induced adverse effect was nullified when the cells were treated with EGCG. In addition, treatment of BM-MSCs with EGCG alone also resulted in the enhancement of osteogenic differentiation of BM-MSCs. After EGCG treatment, expressions of β-catenin and cyclin D1 were upregulated, suggesting that the Wnt pathway was involved in the effects of EGCG on the osteogenic differentiation of BM-MSCs. This was also confirmed by the fact that the Wnt pathway inhibitor, Dickkopf-1 (DKK-1), can nullify the EGCG-induced enhancement effect on BM-MSC’s osteogenic differentiation. Hence, our results suggested that EGCG can reduce the effects of oxidative stress on Wnt pathway in osteogenic cells, which supported a potentially promising therapy of bone disorders induced by oxidative stress. Considering its positive effects on BM-MSCs, EGCG may also be beneficial for stem cell-based bone repair. |
| format | Article |
| id | doaj-art-f8ddc0104d0e40538e7060fc55828410 |
| institution | Kabale University |
| issn | 1687-966X 1687-9678 |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-f8ddc0104d0e40538e7060fc558284102025-02-03T01:11:18ZengWileyStem Cells International1687-966X1687-96782016-01-01201610.1155/2016/75327987532798Epigallocatechin-3-gallate Protects against Hydrogen Peroxide-Induced Inhibition of Osteogenic Differentiation of Human Bone Marrow-Derived Mesenchymal Stem CellsDawei Wang0Yonghui Wang1Shihong Xu2Fu Wang3Bomin Wang4Ke Han5Daqing Sun6Lianxin Li7Department of Orthopedics, Shandong Provincial Hospital Affiliated to Shandong University, Jinan 250021, ChinaDepartment of Orthopedics, Shandong Provincial Hospital Affiliated to Shandong University, Jinan 250021, ChinaDepartment of Orthopedics, Shandong Provincial Hospital Affiliated to Shandong University, Jinan 250021, ChinaDepartment of Orthopedics, Shandong Provincial Hospital Affiliated to Shandong University, Jinan 250021, ChinaDepartment of Orthopedics, Shandong Provincial Hospital Affiliated to Shandong University, Jinan 250021, ChinaDepartment of Orthopedics, Shandong Provincial Hospital Affiliated to Shandong University, Jinan 250021, ChinaDepartment of Vascular Surgery, Second Hospital of Shandong University, Jinan 250000, ChinaDepartment of Orthopedics, Shandong Provincial Hospital Affiliated to Shandong University, Jinan 250021, ChinaOxidative stress induces bone loss and osteoporosis, and epigallocatechin-3-gallate (EGCG) may be used to combat these diseases due to its antioxidative property. Herein, oxidative stress in human bone marrow-derived mesenchymal stem cells (BM-MSCs) was induced by H2O2, resulting in an adverse effect on their osteogenic differentiation. However, this H2O2-induced adverse effect was nullified when the cells were treated with EGCG. In addition, treatment of BM-MSCs with EGCG alone also resulted in the enhancement of osteogenic differentiation of BM-MSCs. After EGCG treatment, expressions of β-catenin and cyclin D1 were upregulated, suggesting that the Wnt pathway was involved in the effects of EGCG on the osteogenic differentiation of BM-MSCs. This was also confirmed by the fact that the Wnt pathway inhibitor, Dickkopf-1 (DKK-1), can nullify the EGCG-induced enhancement effect on BM-MSC’s osteogenic differentiation. Hence, our results suggested that EGCG can reduce the effects of oxidative stress on Wnt pathway in osteogenic cells, which supported a potentially promising therapy of bone disorders induced by oxidative stress. Considering its positive effects on BM-MSCs, EGCG may also be beneficial for stem cell-based bone repair.http://dx.doi.org/10.1155/2016/7532798 |
| spellingShingle | Dawei Wang Yonghui Wang Shihong Xu Fu Wang Bomin Wang Ke Han Daqing Sun Lianxin Li Epigallocatechin-3-gallate Protects against Hydrogen Peroxide-Induced Inhibition of Osteogenic Differentiation of Human Bone Marrow-Derived Mesenchymal Stem Cells Stem Cells International |
| title | Epigallocatechin-3-gallate Protects against Hydrogen Peroxide-Induced Inhibition of Osteogenic Differentiation of Human Bone Marrow-Derived Mesenchymal Stem Cells |
| title_full | Epigallocatechin-3-gallate Protects against Hydrogen Peroxide-Induced Inhibition of Osteogenic Differentiation of Human Bone Marrow-Derived Mesenchymal Stem Cells |
| title_fullStr | Epigallocatechin-3-gallate Protects against Hydrogen Peroxide-Induced Inhibition of Osteogenic Differentiation of Human Bone Marrow-Derived Mesenchymal Stem Cells |
| title_full_unstemmed | Epigallocatechin-3-gallate Protects against Hydrogen Peroxide-Induced Inhibition of Osteogenic Differentiation of Human Bone Marrow-Derived Mesenchymal Stem Cells |
| title_short | Epigallocatechin-3-gallate Protects against Hydrogen Peroxide-Induced Inhibition of Osteogenic Differentiation of Human Bone Marrow-Derived Mesenchymal Stem Cells |
| title_sort | epigallocatechin 3 gallate protects against hydrogen peroxide induced inhibition of osteogenic differentiation of human bone marrow derived mesenchymal stem cells |
| url | http://dx.doi.org/10.1155/2016/7532798 |
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