Utilization of Response Surface Methodology in Optimization of Polysaccharides Extraction from Vietnamese Red Ganoderma lucidum by Ultrasound-Assisted Enzymatic Method and Examination of Bioactivities of the Extract
Red Ganoderma lucidum (G. lucidum) is a popular medicinal herb commonly used in Vietnamese traditional remedies due to its potential value for health. In this study, polysaccharides were extracted from G. lucidum using ultrasound-assisted enzymatic extraction method. The response surface methodology...
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Wiley
2021-01-01
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| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1155/2021/7594092 |
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| author | Dat Tran Do Dang Hoang Lam Tai Nguyen Tran Thi Phuong Mai Le Thao My Phan Hoai Thanh Vuong Duc Viet Nguyen Ngo Thi Thuy Linh Minh Nam Hoang Thanh Phong Mai Huu Hieu Nguyen |
| author_facet | Dat Tran Do Dang Hoang Lam Tai Nguyen Tran Thi Phuong Mai Le Thao My Phan Hoai Thanh Vuong Duc Viet Nguyen Ngo Thi Thuy Linh Minh Nam Hoang Thanh Phong Mai Huu Hieu Nguyen |
| author_sort | Dat Tran Do |
| collection | DOAJ |
| description | Red Ganoderma lucidum (G. lucidum) is a popular medicinal herb commonly used in Vietnamese traditional remedies due to its potential value for health. In this study, polysaccharides were extracted from G. lucidum using ultrasound-assisted enzymatic extraction method. The response surface methodology and Box–Behnken design were employed to investigate the effects of pH, extraction temperature, extraction time, and ultrasonic power on the content of polysaccharides. Based on ultraviolet-visible spectroscopy analysis, the highest content of polysaccharides in the extract was 32.08 mg/g under optimum experimental parameters including enzyme concentration of 3%, pH of 5.5, extraction temperature of 45°C, extraction time of 30 min, and ultrasonic power of 480 W. The Fourier-transform infrared spectroscopy was also used to identify the functional groups in the extracts. The molecular weights of polysaccharides were determined by gel permeation chromatography. The obtained extract was then evaluated for anticancer activities by using (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay, showing the anticancer activities with the half-maximal inhibitory concentration value of more than 512 μg/mL. This result suggested that UAEE could be considered as an appropriate and effective extraction method for bioactive crude polysaccharides from G. lucidum. |
| format | Article |
| id | doaj-art-f8db7dd490854bb1bcd7e5c10e253d7b |
| institution | DOAJ |
| issn | 2356-6140 1537-744X |
| language | English |
| publishDate | 2021-01-01 |
| publisher | Wiley |
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| series | The Scientific World Journal |
| spelling | doaj-art-f8db7dd490854bb1bcd7e5c10e253d7b2025-08-20T03:20:05ZengWileyThe Scientific World Journal2356-61401537-744X2021-01-01202110.1155/2021/75940927594092Utilization of Response Surface Methodology in Optimization of Polysaccharides Extraction from Vietnamese Red Ganoderma lucidum by Ultrasound-Assisted Enzymatic Method and Examination of Bioactivities of the ExtractDat Tran Do0Dang Hoang Lam1Tai Nguyen2Tran Thi Phuong Mai3Le Thao My Phan4Hoai Thanh Vuong5Duc Viet Nguyen6Ngo Thi Thuy Linh7Minh Nam Hoang8Thanh Phong Mai9Huu Hieu Nguyen10VNU-HCMC Key Laboratory of Chemical Engineering and Petroleum Processing (CEPP Lab), Ho Chi Minh City, VietnamFaculty of Chemical Engineering, Ho Chi Minh City University of Technology, 268 Ly Thuong Kiet Street, Ward 14, District 10, Ho Chi Minh City, VietnamFaculty of Chemical Engineering, Ho Chi Minh City University of Technology, 268 Ly Thuong Kiet Street, Ward 14, District 10, Ho Chi Minh City, VietnamFaculty of Chemical Engineering, Ho Chi Minh City University of Technology, 268 Ly Thuong Kiet Street, Ward 14, District 10, Ho Chi Minh City, VietnamVNU-HCMC Key Laboratory of Chemical Engineering and Petroleum Processing (CEPP Lab), Ho Chi Minh City, VietnamVNU-HCMC Key Laboratory of Chemical Engineering and Petroleum Processing (CEPP Lab), Ho Chi Minh City, VietnamFaculty of Chemical Engineering, Ho Chi Minh City University of Technology, 268 Ly Thuong Kiet Street, Ward 14, District 10, Ho Chi Minh City, VietnamVNU-HCMC Key Laboratory of Chemical Engineering and Petroleum Processing (CEPP Lab), Ho Chi Minh City, VietnamFaculty of Chemical Engineering, Ho Chi Minh City University of Technology, 268 Ly Thuong Kiet Street, Ward 14, District 10, Ho Chi Minh City, VietnamFaculty of Chemical Engineering, Ho Chi Minh City University of Technology, 268 Ly Thuong Kiet Street, Ward 14, District 10, Ho Chi Minh City, VietnamVNU-HCMC Key Laboratory of Chemical Engineering and Petroleum Processing (CEPP Lab), Ho Chi Minh City, VietnamRed Ganoderma lucidum (G. lucidum) is a popular medicinal herb commonly used in Vietnamese traditional remedies due to its potential value for health. In this study, polysaccharides were extracted from G. lucidum using ultrasound-assisted enzymatic extraction method. The response surface methodology and Box–Behnken design were employed to investigate the effects of pH, extraction temperature, extraction time, and ultrasonic power on the content of polysaccharides. Based on ultraviolet-visible spectroscopy analysis, the highest content of polysaccharides in the extract was 32.08 mg/g under optimum experimental parameters including enzyme concentration of 3%, pH of 5.5, extraction temperature of 45°C, extraction time of 30 min, and ultrasonic power of 480 W. The Fourier-transform infrared spectroscopy was also used to identify the functional groups in the extracts. The molecular weights of polysaccharides were determined by gel permeation chromatography. The obtained extract was then evaluated for anticancer activities by using (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay, showing the anticancer activities with the half-maximal inhibitory concentration value of more than 512 μg/mL. This result suggested that UAEE could be considered as an appropriate and effective extraction method for bioactive crude polysaccharides from G. lucidum.http://dx.doi.org/10.1155/2021/7594092 |
| spellingShingle | Dat Tran Do Dang Hoang Lam Tai Nguyen Tran Thi Phuong Mai Le Thao My Phan Hoai Thanh Vuong Duc Viet Nguyen Ngo Thi Thuy Linh Minh Nam Hoang Thanh Phong Mai Huu Hieu Nguyen Utilization of Response Surface Methodology in Optimization of Polysaccharides Extraction from Vietnamese Red Ganoderma lucidum by Ultrasound-Assisted Enzymatic Method and Examination of Bioactivities of the Extract The Scientific World Journal |
| title | Utilization of Response Surface Methodology in Optimization of Polysaccharides Extraction from Vietnamese Red Ganoderma lucidum by Ultrasound-Assisted Enzymatic Method and Examination of Bioactivities of the Extract |
| title_full | Utilization of Response Surface Methodology in Optimization of Polysaccharides Extraction from Vietnamese Red Ganoderma lucidum by Ultrasound-Assisted Enzymatic Method and Examination of Bioactivities of the Extract |
| title_fullStr | Utilization of Response Surface Methodology in Optimization of Polysaccharides Extraction from Vietnamese Red Ganoderma lucidum by Ultrasound-Assisted Enzymatic Method and Examination of Bioactivities of the Extract |
| title_full_unstemmed | Utilization of Response Surface Methodology in Optimization of Polysaccharides Extraction from Vietnamese Red Ganoderma lucidum by Ultrasound-Assisted Enzymatic Method and Examination of Bioactivities of the Extract |
| title_short | Utilization of Response Surface Methodology in Optimization of Polysaccharides Extraction from Vietnamese Red Ganoderma lucidum by Ultrasound-Assisted Enzymatic Method and Examination of Bioactivities of the Extract |
| title_sort | utilization of response surface methodology in optimization of polysaccharides extraction from vietnamese red ganoderma lucidum by ultrasound assisted enzymatic method and examination of bioactivities of the extract |
| url | http://dx.doi.org/10.1155/2021/7594092 |
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