Studies of biological properties of continuous suspension ВНК-21/SUSP/ARRIAH cell line
The results of the studies of cytomorphological, karyological, cultural properties of continuous suspension ВНК-21/SUSP/ARRIAH subline of newborn Syrian hamster kidney cells intended for foot-and-mouth disease, rabies, bovine parainfluenza-3, Aujeszky’s disease virus reproduction, as well as for pro...
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Da Vinci Media
2021-08-01
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| Series: | Ветеринария сегодня |
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| Online Access: | https://veterinary.arriah.ru/jour/article/view/576 |
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| author | M. I. Doronin M. N. Guseva D. V. Mikhalishin A. S. Sharypov N. S. Mudrak N. Ye. Kamalova B. L. Manin |
| author_facet | M. I. Doronin M. N. Guseva D. V. Mikhalishin A. S. Sharypov N. S. Mudrak N. Ye. Kamalova B. L. Manin |
| author_sort | M. I. Doronin |
| collection | DOAJ |
| description | The results of the studies of cytomorphological, karyological, cultural properties of continuous suspension ВНК-21/SUSP/ARRIAH subline of newborn Syrian hamster kidney cells intended for foot-and-mouth disease, rabies, bovine parainfluenza-3, Aujeszky’s disease virus reproduction, as well as for production of diagnostic veterinary biologicals are presented. When cultured in suspension, BHK-21/SUSP/ARRIAH cell subline undergoes selection towards hypoploidy: modal class is represented by cells with 41 chromosomes (32–40% of cells); the share of cells containing 40–42 chromosomes is 78–80%; the share of polyploids averages around 1%; the range of variation in the number of chromosomes is from 36 to 54. BHK-21/SUSP/ARRIAH cell subline cultured in suspension with cell seeding concentration of 0.6–0.8 million cells/cm3 demonstrates growth rate of 6.67–11.00 and 96–99% cell viability. After 48 hours, G1-phase (diploid-2n) cells prevail in the cell population of the new subline (30.0–75.0% of cells); cells that undergo preparation for mitosis (S-phase) and mitosis (G2+M-phase) account for 3.0 to 20.0% of the entire population; the number of meganucleated and multinucleated cells (>4n) at the beginning and at the end of the logarithmic phase increases to 2%. BHK-21/SUSP/ARRIAH cells recover rapidly after cryopreservation and demonstrate 95–99% viability and growth rate of 3.36–5.88 at passages 1 to 3 and 6.85–10.95 at passages 4 to 12. Continuous suspension BHK-21/SUSP/ARRIAH cell line ensures virus accumulation at the following titres: FMD virus – 7.30– 8.00 lg TCID50/cm3, rabies virus – 7.25–8.00 lg CCID50/cm3, bovine parainflunza-3 virus – at least 6.00 lg TCID50/cm3, Aujeszky’s disease virus – 7.50–7.80 lg TCID50/cm3. |
| format | Article |
| id | doaj-art-f8d81ca01ce14536a8688266c9652915 |
| institution | Kabale University |
| issn | 2304-196X 2658-6959 |
| language | English |
| publishDate | 2021-08-01 |
| publisher | Da Vinci Media |
| record_format | Article |
| series | Ветеринария сегодня |
| spelling | doaj-art-f8d81ca01ce14536a8688266c96529152025-02-06T09:52:08ZengDa Vinci MediaВетеринария сегодня2304-196X2658-69592021-08-0110323023810.29326/2304-196X-2021-3-38-230-238523Studies of biological properties of continuous suspension ВНК-21/SUSP/ARRIAH cell lineM. I. Doronin0M. N. Guseva1D. V. Mikhalishin2A. S. Sharypov3N. S. Mudrak4N. Ye. Kamalova5B. L. Manin6FGBI “Federal Centre for Animal Health” (FGBI “ARRIAH”)FGBI “Federal Centre for Animal Health” (FGBI “ARRIAH”)FGBI “Federal Centre for Animal Health” (FGBI “ARRIAH”)FSBI “Central Scientific and Methodological Veterinary Laboratory” (FSBI CNMVL)FGBI “Federal Centre for Animal Health” (FGBI “ARRIAH”)FGBI “Federal Centre for Animal Health” (FGBI “ARRIAH”)FGBI “Federal Centre for Animal Health” (FGBI “ARRIAH”)The results of the studies of cytomorphological, karyological, cultural properties of continuous suspension ВНК-21/SUSP/ARRIAH subline of newborn Syrian hamster kidney cells intended for foot-and-mouth disease, rabies, bovine parainfluenza-3, Aujeszky’s disease virus reproduction, as well as for production of diagnostic veterinary biologicals are presented. When cultured in suspension, BHK-21/SUSP/ARRIAH cell subline undergoes selection towards hypoploidy: modal class is represented by cells with 41 chromosomes (32–40% of cells); the share of cells containing 40–42 chromosomes is 78–80%; the share of polyploids averages around 1%; the range of variation in the number of chromosomes is from 36 to 54. BHK-21/SUSP/ARRIAH cell subline cultured in suspension with cell seeding concentration of 0.6–0.8 million cells/cm3 demonstrates growth rate of 6.67–11.00 and 96–99% cell viability. After 48 hours, G1-phase (diploid-2n) cells prevail in the cell population of the new subline (30.0–75.0% of cells); cells that undergo preparation for mitosis (S-phase) and mitosis (G2+M-phase) account for 3.0 to 20.0% of the entire population; the number of meganucleated and multinucleated cells (>4n) at the beginning and at the end of the logarithmic phase increases to 2%. BHK-21/SUSP/ARRIAH cells recover rapidly after cryopreservation and demonstrate 95–99% viability and growth rate of 3.36–5.88 at passages 1 to 3 and 6.85–10.95 at passages 4 to 12. Continuous suspension BHK-21/SUSP/ARRIAH cell line ensures virus accumulation at the following titres: FMD virus – 7.30– 8.00 lg TCID50/cm3, rabies virus – 7.25–8.00 lg CCID50/cm3, bovine parainflunza-3 virus – at least 6.00 lg TCID50/cm3, Aujeszky’s disease virus – 7.50–7.80 lg TCID50/cm3.https://veterinary.arriah.ru/jour/article/view/576внк-21/susp/arriah cell linebiological properties of cell culturefoot-and-mouth diseaserabiesbovine parainflunza-3aujeszky’s disease |
| spellingShingle | M. I. Doronin M. N. Guseva D. V. Mikhalishin A. S. Sharypov N. S. Mudrak N. Ye. Kamalova B. L. Manin Studies of biological properties of continuous suspension ВНК-21/SUSP/ARRIAH cell line Ветеринария сегодня внк-21/susp/arriah cell line biological properties of cell culture foot-and-mouth disease rabies bovine parainflunza-3 aujeszky’s disease |
| title | Studies of biological properties of continuous suspension ВНК-21/SUSP/ARRIAH cell line |
| title_full | Studies of biological properties of continuous suspension ВНК-21/SUSP/ARRIAH cell line |
| title_fullStr | Studies of biological properties of continuous suspension ВНК-21/SUSP/ARRIAH cell line |
| title_full_unstemmed | Studies of biological properties of continuous suspension ВНК-21/SUSP/ARRIAH cell line |
| title_short | Studies of biological properties of continuous suspension ВНК-21/SUSP/ARRIAH cell line |
| title_sort | studies of biological properties of continuous suspension внк 21 susp arriah cell line |
| topic | внк-21/susp/arriah cell line biological properties of cell culture foot-and-mouth disease rabies bovine parainflunza-3 aujeszky’s disease |
| url | https://veterinary.arriah.ru/jour/article/view/576 |
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