Virus-induced gene silencing
The term virus-induced gene silencing' (VIGS) was first used to describe the phenomenon of recovery from virus infection, a natural plant antiviral mechanism, which recently has been exploited as a genetic technique involving recombinant viruses with a target gene fragment insert to knock down...
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| Format: | Article |
| Language: | English |
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Zhejiang University Press
2008-03-01
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| Series: | 浙江大学学报. 农业与生命科学版 |
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| Online Access: | https://www.academax.com/doi/10.3785/j.issn.1008-9209.2008.02.001 |
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| _version_ | 1850052361378070528 |
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| author | XU You-ping XU Qiu-fang SONG Xiao-yi ZHANG Zhi-xin CAI Xin-zhong |
| author_facet | XU You-ping XU Qiu-fang SONG Xiao-yi ZHANG Zhi-xin CAI Xin-zhong |
| author_sort | XU You-ping |
| collection | DOAJ |
| description | The term virus-induced gene silencing' (VIGS) was first used to describe the phenomenon of recovery from virus infection, a natural plant antiviral mechanism, which recently has been exploited as a genetic technique involving recombinant viruses with a target gene fragment insert to knock down expression of endogenous target genes, and thereby analyse gene functions. VIGS is triggered by small interfering RNA (siRNA), whose single strand associates with RNA-induced silencing complex (RISC), which specifically binds to and thereafter degrades target RNA that is homologous to the siRNA. Fifteen VIGS vectors have been exploited from different types of viruses. The VIGS efficiency is significantly affected by the fragment inserted into the vector, the method for vector delivery, the host developmental stage when used for VIGS induction and the growth conditions of plants after VIGS induction treatment. As a novel technical tool for gene identification and functional analysis, VIGS has many advantages such as no necessity to know full-length sequences of the target genes, rapidness to obtain phenotype and avoiding construction of transgenic plants, and thus has been applying more and more broadly and extensively to plant gene functional analysis. |
| format | Article |
| id | doaj-art-f8c80e95501c4e99a94011fac7b0b05d |
| institution | DOAJ |
| issn | 1008-9209 2097-5155 |
| language | English |
| publishDate | 2008-03-01 |
| publisher | Zhejiang University Press |
| record_format | Article |
| series | 浙江大学学报. 农业与生命科学版 |
| spelling | doaj-art-f8c80e95501c4e99a94011fac7b0b05d2025-08-20T02:52:49ZengZhejiang University Press浙江大学学报. 农业与生命科学版1008-92092097-51552008-03-013411913110.3785/j.issn.1008-9209.2008.02.00110089209Virus-induced gene silencingXU You-pingXU Qiu-fangSONG Xiao-yiZHANG Zhi-xinCAI Xin-zhongThe term virus-induced gene silencing' (VIGS) was first used to describe the phenomenon of recovery from virus infection, a natural plant antiviral mechanism, which recently has been exploited as a genetic technique involving recombinant viruses with a target gene fragment insert to knock down expression of endogenous target genes, and thereby analyse gene functions. VIGS is triggered by small interfering RNA (siRNA), whose single strand associates with RNA-induced silencing complex (RISC), which specifically binds to and thereafter degrades target RNA that is homologous to the siRNA. Fifteen VIGS vectors have been exploited from different types of viruses. The VIGS efficiency is significantly affected by the fragment inserted into the vector, the method for vector delivery, the host developmental stage when used for VIGS induction and the growth conditions of plants after VIGS induction treatment. As a novel technical tool for gene identification and functional analysis, VIGS has many advantages such as no necessity to know full-length sequences of the target genes, rapidness to obtain phenotype and avoiding construction of transgenic plants, and thus has been applying more and more broadly and extensively to plant gene functional analysis.https://www.academax.com/doi/10.3785/j.issn.1008-9209.2008.02.001virus-induced gene silencingmechanismvectorsilence efficiencyfunctional genomics |
| spellingShingle | XU You-ping XU Qiu-fang SONG Xiao-yi ZHANG Zhi-xin CAI Xin-zhong Virus-induced gene silencing 浙江大学学报. 农业与生命科学版 virus-induced gene silencing mechanism vector silence efficiency functional genomics |
| title | Virus-induced gene silencing |
| title_full | Virus-induced gene silencing |
| title_fullStr | Virus-induced gene silencing |
| title_full_unstemmed | Virus-induced gene silencing |
| title_short | Virus-induced gene silencing |
| title_sort | virus induced gene silencing |
| topic | virus-induced gene silencing mechanism vector silence efficiency functional genomics |
| url | https://www.academax.com/doi/10.3785/j.issn.1008-9209.2008.02.001 |
| work_keys_str_mv | AT xuyouping virusinducedgenesilencing AT xuqiufang virusinducedgenesilencing AT songxiaoyi virusinducedgenesilencing AT zhangzhixin virusinducedgenesilencing AT caixinzhong virusinducedgenesilencing |