Differential Analysis of Fecal SCFAs and Their Contribution to Adipogenesis in UCP1 Knock-In Pigs
This study aimed to investigate the changes in fecal short-chain fatty acids (SCFAs) content in UCP1 knock-in pigs (KI pigs) and their effect on adipogenesis. Fecal samples from five 6-month-old wild-type (WT) and KI pigs were collected for targeted metabolomics and 16s rRNA sequencing analyses to i...
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MDPI AG
2025-02-01
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| Series: | Veterinary Sciences |
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| author | Chengyu Zhao Jianfei Pan Yanfang Wang Jianguo Zhao Jiaojiao Huang |
| author_facet | Chengyu Zhao Jianfei Pan Yanfang Wang Jianguo Zhao Jiaojiao Huang |
| author_sort | Chengyu Zhao |
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| description | This study aimed to investigate the changes in fecal short-chain fatty acids (SCFAs) content in UCP1 knock-in pigs (KI pigs) and their effect on adipogenesis. Fecal samples from five 6-month-old wild-type (WT) and KI pigs were collected for targeted metabolomics and 16s rRNA sequencing analyses to identify differences in SCFAs and gut microbiota that may contribute to regulating fat deposition in pigs. The metabolome of pig fecal samples targeted for an analysis of SCFAs identified seven SCFAs, with caproic acid (except isovaleric acid) being the significantly different one. The results of the fecal 16s rRNA analysis demonstrated a notable reduction in the abundance of <i>Streptococcus</i> spp. in the KI pigs in comparison to the WT pigs, with a statistically significant difference. Correlation analyses demonstrated a statistically significant positive correlation between the abundance of <i>Streptococcus</i> spp. and SCFAs, as well as pig body weight and fatness. It was postulated that the reduction in SCFAs in the intestinal tracts of KI pigs may be associated with a reduction in <i>Streptococcus</i> spp. abundance. Compared to WT pigs, the concentration of fecal SCFAs in KI pigs was significantly reduced, which may be related to the decreased abundance of <i>Streptococcus</i>. The in vitro experiments showed that caproic acid could significantly enhance the differentiation efficiency of porcine SVF cells into mature adipocytes by activating the <i>FFAR4</i> gene. |
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| institution | DOAJ |
| issn | 2306-7381 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | MDPI AG |
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| spelling | doaj-art-f8bbdabbb43a4d4f8bc6fa941c94f02f2025-08-20T03:12:10ZengMDPI AGVeterinary Sciences2306-73812025-02-0112210210.3390/vetsci12020102Differential Analysis of Fecal SCFAs and Their Contribution to Adipogenesis in UCP1 Knock-In PigsChengyu Zhao0Jianfei Pan1Yanfang Wang2Jianguo Zhao3Jiaojiao Huang4College of Animal Science and Technology, Qingdao Agricultural University, Qingdao 266109, ChinaState Key Laboratory of Animal Biotech Breeding, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, ChinaState Key Laboratory of Animal Biotech Breeding, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, ChinaState Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, ChinaCollege of Animal Science and Technology, Qingdao Agricultural University, Qingdao 266109, ChinaThis study aimed to investigate the changes in fecal short-chain fatty acids (SCFAs) content in UCP1 knock-in pigs (KI pigs) and their effect on adipogenesis. Fecal samples from five 6-month-old wild-type (WT) and KI pigs were collected for targeted metabolomics and 16s rRNA sequencing analyses to identify differences in SCFAs and gut microbiota that may contribute to regulating fat deposition in pigs. The metabolome of pig fecal samples targeted for an analysis of SCFAs identified seven SCFAs, with caproic acid (except isovaleric acid) being the significantly different one. The results of the fecal 16s rRNA analysis demonstrated a notable reduction in the abundance of <i>Streptococcus</i> spp. in the KI pigs in comparison to the WT pigs, with a statistically significant difference. Correlation analyses demonstrated a statistically significant positive correlation between the abundance of <i>Streptococcus</i> spp. and SCFAs, as well as pig body weight and fatness. It was postulated that the reduction in SCFAs in the intestinal tracts of KI pigs may be associated with a reduction in <i>Streptococcus</i> spp. abundance. Compared to WT pigs, the concentration of fecal SCFAs in KI pigs was significantly reduced, which may be related to the decreased abundance of <i>Streptococcus</i>. The in vitro experiments showed that caproic acid could significantly enhance the differentiation efficiency of porcine SVF cells into mature adipocytes by activating the <i>FFAR4</i> gene.https://www.mdpi.com/2306-7381/12/2/102pigs<i>UCP1</i>SCFAscaproic acidfat depositiongut microbiota |
| spellingShingle | Chengyu Zhao Jianfei Pan Yanfang Wang Jianguo Zhao Jiaojiao Huang Differential Analysis of Fecal SCFAs and Their Contribution to Adipogenesis in UCP1 Knock-In Pigs Veterinary Sciences pigs <i>UCP1</i> SCFAs caproic acid fat deposition gut microbiota |
| title | Differential Analysis of Fecal SCFAs and Their Contribution to Adipogenesis in UCP1 Knock-In Pigs |
| title_full | Differential Analysis of Fecal SCFAs and Their Contribution to Adipogenesis in UCP1 Knock-In Pigs |
| title_fullStr | Differential Analysis of Fecal SCFAs and Their Contribution to Adipogenesis in UCP1 Knock-In Pigs |
| title_full_unstemmed | Differential Analysis of Fecal SCFAs and Their Contribution to Adipogenesis in UCP1 Knock-In Pigs |
| title_short | Differential Analysis of Fecal SCFAs and Their Contribution to Adipogenesis in UCP1 Knock-In Pigs |
| title_sort | differential analysis of fecal scfas and their contribution to adipogenesis in ucp1 knock in pigs |
| topic | pigs <i>UCP1</i> SCFAs caproic acid fat deposition gut microbiota |
| url | https://www.mdpi.com/2306-7381/12/2/102 |
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