Molecular Prevalence and Phylogenetic Analysis of <i>Anaplasma</i> spp. in Goats from Adana, Türkiye
Anaplasmosis is a disease in animals that leads to significant economic losses. In addition, the zoonotic potential of vector-borne <i>Anaplasma</i> species is increasing its importance, both around the world and in Türkiye, in particular. The aim of this study was to determine the preva...
Saved in:
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2025-05-01
|
| Series: | Veterinary Sciences |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2306-7381/12/5/481 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849718926934540288 |
|---|---|
| author | Tülin Güven Gökmen Armağan Erdem Ütük Esra Aslan Tokgöz Nur Sima Uprak Afra Sena Tekin Ufuk Erol Pınar Ayvazoğlu Demir Osman Sezer Elçin Günaydın |
| author_facet | Tülin Güven Gökmen Armağan Erdem Ütük Esra Aslan Tokgöz Nur Sima Uprak Afra Sena Tekin Ufuk Erol Pınar Ayvazoğlu Demir Osman Sezer Elçin Günaydın |
| author_sort | Tülin Güven Gökmen |
| collection | DOAJ |
| description | Anaplasmosis is a disease in animals that leads to significant economic losses. In addition, the zoonotic potential of vector-borne <i>Anaplasma</i> species is increasing its importance, both around the world and in Türkiye, in particular. The aim of this study was to determine the prevalence and phylogenetic profile of <i>Anaplasma</i> species in goats from the districts of Adana province, Türkiye, via molecular methods. For this purpose, blood samples from 364 goats of various breeds, ages, and genders were collected, and the presence of 55 <i>Anaplasma</i> spp. was determined through the PCR method. <i>Anaplasma ovis</i> was detected in 55 samples (15.1%) and <i>A. phagocytophilum</i> in 11 samples (3%) by Nested-PCR. However, <i>A. capra</i> was not detected. The phylogenetic relationships of <i>A. ovis</i> and <i>A. phagocytophilum</i> were investigated by DNA sequencing of the <i>msp4</i> and <i>16S rRNA</i> gene regions. Through sequence analysis in <i>A. phagocytophilum</i>, we identified nine isolates as <i>A. phagocytophilum</i>-like 1 and one isolate as <i>A. phagocytophilum</i>-like 2. Forty-six of the <i>A. ovis</i> isolates were 100% similar to each other. The other 2 isolates were also 100% similar to each other but had a single nucleotide difference from the other 46 isolates. Notably, the isolate identified as <i>A. phagocytophilum</i>-like 2 was detected for the first time in goats in Türkiye. |
| format | Article |
| id | doaj-art-f8a810726db7494bb3e1e4dfce78d009 |
| institution | DOAJ |
| issn | 2306-7381 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Veterinary Sciences |
| spelling | doaj-art-f8a810726db7494bb3e1e4dfce78d0092025-08-20T03:12:15ZengMDPI AGVeterinary Sciences2306-73812025-05-0112548110.3390/vetsci12050481Molecular Prevalence and Phylogenetic Analysis of <i>Anaplasma</i> spp. in Goats from Adana, TürkiyeTülin Güven Gökmen0Armağan Erdem Ütük1Esra Aslan Tokgöz2Nur Sima Uprak3Afra Sena Tekin4Ufuk Erol5Pınar Ayvazoğlu Demir6Osman Sezer7Elçin Günaydın8Department of Microbiology, Ceyhan Veterinary Faculty, Çukurova University, Adana 01330, TürkiyeDepartment of Parasitology, Ceyhan Veterinary Faculty, Çukurova University, Adana 01330, TürkiyeAdana Veterinary Control Institute, Adana 01122, TürkiyeDepartment of Microbiology, Ceyhan Veterinary Faculty, Çukurova University, Adana 01330, TürkiyeDepartment of Parasitology, Ceyhan Veterinary Faculty, Çukurova University, Adana 01330, TürkiyeDepartment of Parasitology, Veterinary Faculty, Sivas Cumhuriyet University, Sivas 58140, TürkiyeDepartment of Animal Health Economics and Management, Veterinary Faculty, Kırıkkale University, Kırıkkale 71450, TürkiyeAdana Veterinary Control Institute, Adana 01122, TürkiyeDepartment of Microbiology, Veterinary Faculty, Kastamonu University, Kastamonu 37150, TürkiyeAnaplasmosis is a disease in animals that leads to significant economic losses. In addition, the zoonotic potential of vector-borne <i>Anaplasma</i> species is increasing its importance, both around the world and in Türkiye, in particular. The aim of this study was to determine the prevalence and phylogenetic profile of <i>Anaplasma</i> species in goats from the districts of Adana province, Türkiye, via molecular methods. For this purpose, blood samples from 364 goats of various breeds, ages, and genders were collected, and the presence of 55 <i>Anaplasma</i> spp. was determined through the PCR method. <i>Anaplasma ovis</i> was detected in 55 samples (15.1%) and <i>A. phagocytophilum</i> in 11 samples (3%) by Nested-PCR. However, <i>A. capra</i> was not detected. The phylogenetic relationships of <i>A. ovis</i> and <i>A. phagocytophilum</i> were investigated by DNA sequencing of the <i>msp4</i> and <i>16S rRNA</i> gene regions. Through sequence analysis in <i>A. phagocytophilum</i>, we identified nine isolates as <i>A. phagocytophilum</i>-like 1 and one isolate as <i>A. phagocytophilum</i>-like 2. Forty-six of the <i>A. ovis</i> isolates were 100% similar to each other. The other 2 isolates were also 100% similar to each other but had a single nucleotide difference from the other 46 isolates. Notably, the isolate identified as <i>A. phagocytophilum</i>-like 2 was detected for the first time in goats in Türkiye.https://www.mdpi.com/2306-7381/12/5/481<i>A. ovis</i><i>A. phagocytophilum</i>-like 1<i>A. phagocytophilum</i>-like 2<i>A. capra</i>goat |
| spellingShingle | Tülin Güven Gökmen Armağan Erdem Ütük Esra Aslan Tokgöz Nur Sima Uprak Afra Sena Tekin Ufuk Erol Pınar Ayvazoğlu Demir Osman Sezer Elçin Günaydın Molecular Prevalence and Phylogenetic Analysis of <i>Anaplasma</i> spp. in Goats from Adana, Türkiye Veterinary Sciences <i>A. ovis</i> <i>A. phagocytophilum</i>-like 1 <i>A. phagocytophilum</i>-like 2 <i>A. capra</i> goat |
| title | Molecular Prevalence and Phylogenetic Analysis of <i>Anaplasma</i> spp. in Goats from Adana, Türkiye |
| title_full | Molecular Prevalence and Phylogenetic Analysis of <i>Anaplasma</i> spp. in Goats from Adana, Türkiye |
| title_fullStr | Molecular Prevalence and Phylogenetic Analysis of <i>Anaplasma</i> spp. in Goats from Adana, Türkiye |
| title_full_unstemmed | Molecular Prevalence and Phylogenetic Analysis of <i>Anaplasma</i> spp. in Goats from Adana, Türkiye |
| title_short | Molecular Prevalence and Phylogenetic Analysis of <i>Anaplasma</i> spp. in Goats from Adana, Türkiye |
| title_sort | molecular prevalence and phylogenetic analysis of i anaplasma i spp in goats from adana turkiye |
| topic | <i>A. ovis</i> <i>A. phagocytophilum</i>-like 1 <i>A. phagocytophilum</i>-like 2 <i>A. capra</i> goat |
| url | https://www.mdpi.com/2306-7381/12/5/481 |
| work_keys_str_mv | AT tulinguvengokmen molecularprevalenceandphylogeneticanalysisofianaplasmaisppingoatsfromadanaturkiye AT armaganerdemutuk molecularprevalenceandphylogeneticanalysisofianaplasmaisppingoatsfromadanaturkiye AT esraaslantokgoz molecularprevalenceandphylogeneticanalysisofianaplasmaisppingoatsfromadanaturkiye AT nursimauprak molecularprevalenceandphylogeneticanalysisofianaplasmaisppingoatsfromadanaturkiye AT afrasenatekin molecularprevalenceandphylogeneticanalysisofianaplasmaisppingoatsfromadanaturkiye AT ufukerol molecularprevalenceandphylogeneticanalysisofianaplasmaisppingoatsfromadanaturkiye AT pınarayvazogludemir molecularprevalenceandphylogeneticanalysisofianaplasmaisppingoatsfromadanaturkiye AT osmansezer molecularprevalenceandphylogeneticanalysisofianaplasmaisppingoatsfromadanaturkiye AT elcingunaydın molecularprevalenceandphylogeneticanalysisofianaplasmaisppingoatsfromadanaturkiye |