Determination of secoisolariciresinol diglucoside by high performance liquid chromatography
High performance liquid chromatography-photodiode array detection-elctrospray ionizationmass spectrometry (HPLC-PAD-ESI-MS) method was used to determine secoisolariciresinol diglucoside (SDG) qualitatively and quantitatively. Precision, stability, limits of quantification, SDG recovery and sample pr...
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| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Zhejiang University Press
2008-09-01
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| Series: | 浙江大学学报. 农业与生命科学版 |
| Subjects: | |
| Online Access: | https://www.academax.com/doi/10.3785/j.issn.1008-9209.2008.05.013 |
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| Summary: | High performance liquid chromatography-photodiode array detection-elctrospray ionizationmass spectrometry (HPLC-PAD-ESI-MS) method was used to determine secoisolariciresinol diglucoside (SDG) qualitatively and quantitatively. Precision, stability, limits of quantification, SDG recovery and sample preparation method were studied. SDG was isolated on a Sephadex LH-20 column chromatography and characterized by <sup>1</sup>H-NMR and <sup>13</sup>C-NMR. Calibration curve was built with the purified SDG (with purity of 96.6%) as a standard. The UV spectrum and ESI-MS spectra were in accordance with SDG structure. The HPLC-PAD-ESI-MS method had high precision (RSD 1.36%), good stability (RSD 1.21% after a week), low limit of quantification (3.4 mg·L<sup>-1</sup>) and satisfying recovery (99.7 ± 3.2)%. In addition, by combination of solvent extraction with alkaline hydrolysis, sample preparation time was saved and SDG yield was improved. This HPLC-PAD-ESI-MS method is convenient, time-saving, accurate and reliable. |
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| ISSN: | 1008-9209 2097-5155 |