Optimization of phenol biodegradation by efficient bacteria isolated from petrochemical effluents
Phenol is an environmental pollutant present in industrial wastewaters such as refineries, coal processing and petrochemicals products. In this study three phenol degrading bacteria from Arak Petrochemical Complex effluent were isolated which consume phenol. Molecular analysis was used to identify b...
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GJESM Publisher
2016-05-01
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| Series: | Global Journal of Environmental Science and Management |
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| Online Access: | http://www.gjesm.net/article_19480_b66717645c3445545c42611396b170aa.pdf |
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| author | M. Shahriari Moghadam N. Safaei G.H. Ebrahimipour |
| author_facet | M. Shahriari Moghadam N. Safaei G.H. Ebrahimipour |
| author_sort | M. Shahriari Moghadam |
| collection | DOAJ |
| description | Phenol is an environmental pollutant present in industrial wastewaters such as refineries, coal processing and petrochemicals products. In this study three phenol degrading bacteria from Arak Petrochemical Complex effluent were isolated which consume phenol. Molecular analysis was used to identify bacteria and isolated bacteria were identified as Rhodococcus pyridinivorans (NS1), Advenella faeciporci (NS2) and Pseudomonas aeroginosa (NS3). Among the isolated strains, NS1 had the highest ability to degrade phenol. In order to observe the best yield in phenol biodegradation using NS1, optimization was performed using one factor at a time of experimental design to investigate the effect of four factors, including pH, temperature, phosphate and urea concentration. The optimal biodegradation condition through or tho pathway was pH = 8, urea = 1 g/L, temperature = 30°C and K2HPO4 = 0.5 g/L. Under the suggested condition, a biodegradation efficiency of 100% was achieved. Moreover, NS1 has shown growth and phenol degradation in concentrations between 250 to 2000 mg/L. In a nutshell, the results revealed thatphenol efficiently consumed by NS1 as the sole carbon source. Obviously, the isolate strain may be seen as an important tool in the bioremediation of wastewater effluent, petrochemical complex. |
| format | Article |
| id | doaj-art-f7e3cc8d1ef44ee89066549a0df9b3f3 |
| institution | Kabale University |
| issn | 2383-3572 2383-3866 |
| language | English |
| publishDate | 2016-05-01 |
| publisher | GJESM Publisher |
| record_format | Article |
| series | Global Journal of Environmental Science and Management |
| spelling | doaj-art-f7e3cc8d1ef44ee89066549a0df9b3f32025-02-02T01:16:29ZengGJESM PublisherGlobal Journal of Environmental Science and Management2383-35722383-38662016-05-012324925610.7508/gjesm.2016.03.00419480Optimization of phenol biodegradation by efficient bacteria isolated from petrochemical effluentsM. Shahriari Moghadam0N. Safaei1G.H. Ebrahimipour2Department of the Environment, Faculty of Natural Resources, University of Zabol, Zabol, IranDepartment of Microbiology, Faculty of Biological Science, University of Shahid Beheshti, Tehran, IranDepartment of Microbiology, Faculty of Biological Science, University of Shahid Beheshti, Tehran, IranPhenol is an environmental pollutant present in industrial wastewaters such as refineries, coal processing and petrochemicals products. In this study three phenol degrading bacteria from Arak Petrochemical Complex effluent were isolated which consume phenol. Molecular analysis was used to identify bacteria and isolated bacteria were identified as Rhodococcus pyridinivorans (NS1), Advenella faeciporci (NS2) and Pseudomonas aeroginosa (NS3). Among the isolated strains, NS1 had the highest ability to degrade phenol. In order to observe the best yield in phenol biodegradation using NS1, optimization was performed using one factor at a time of experimental design to investigate the effect of four factors, including pH, temperature, phosphate and urea concentration. The optimal biodegradation condition through or tho pathway was pH = 8, urea = 1 g/L, temperature = 30°C and K2HPO4 = 0.5 g/L. Under the suggested condition, a biodegradation efficiency of 100% was achieved. Moreover, NS1 has shown growth and phenol degradation in concentrations between 250 to 2000 mg/L. In a nutshell, the results revealed thatphenol efficiently consumed by NS1 as the sole carbon source. Obviously, the isolate strain may be seen as an important tool in the bioremediation of wastewater effluent, petrochemical complex.http://www.gjesm.net/article_19480_b66717645c3445545c42611396b170aa.pdfBiodegradationEffluentsOptimiztionPetrochemical effluentPhenolRhodococcus pyridinovorans |
| spellingShingle | M. Shahriari Moghadam N. Safaei G.H. Ebrahimipour Optimization of phenol biodegradation by efficient bacteria isolated from petrochemical effluents Global Journal of Environmental Science and Management Biodegradation Effluents Optimiztion Petrochemical effluent Phenol Rhodococcus pyridinovorans |
| title | Optimization of phenol biodegradation by efficient bacteria isolated from petrochemical effluents |
| title_full | Optimization of phenol biodegradation by efficient bacteria isolated from petrochemical effluents |
| title_fullStr | Optimization of phenol biodegradation by efficient bacteria isolated from petrochemical effluents |
| title_full_unstemmed | Optimization of phenol biodegradation by efficient bacteria isolated from petrochemical effluents |
| title_short | Optimization of phenol biodegradation by efficient bacteria isolated from petrochemical effluents |
| title_sort | optimization of phenol biodegradation by efficient bacteria isolated from petrochemical effluents |
| topic | Biodegradation Effluents Optimiztion Petrochemical effluent Phenol Rhodococcus pyridinovorans |
| url | http://www.gjesm.net/article_19480_b66717645c3445545c42611396b170aa.pdf |
| work_keys_str_mv | AT mshahriarimoghadam optimizationofphenolbiodegradationbyefficientbacteriaisolatedfrompetrochemicaleffluents AT nsafaei optimizationofphenolbiodegradationbyefficientbacteriaisolatedfrompetrochemicaleffluents AT ghebrahimipour optimizationofphenolbiodegradationbyefficientbacteriaisolatedfrompetrochemicaleffluents |