Seminal F<sub>2</sub>-IsoP and RvD1 Levels in Idiopathic Infertile Men
30 percent of infertile men are diagnosed with idiopathic infertility. This study aimed to assess oxidative stress in the semen of 77 patients with idiopathic infertility by measuring F<sub>2</sub>-isoprostane (F<sub>2</sub>-IsoP), resolvin D1 (RvD1) levels, and semen paramet...
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2025-04-01
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| author | Elena Moretti Giulia Collodel Caterina Marcucci Laura Liguori Laura Gambera Cinzia Signorini |
| author_facet | Elena Moretti Giulia Collodel Caterina Marcucci Laura Liguori Laura Gambera Cinzia Signorini |
| author_sort | Elena Moretti |
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| description | 30 percent of infertile men are diagnosed with idiopathic infertility. This study aimed to assess oxidative stress in the semen of 77 patients with idiopathic infertility by measuring F<sub>2</sub>-isoprostane (F<sub>2</sub>-IsoP), resolvin D1 (RvD1) levels, and semen parameters. The presence and localization of 8-IsoProstaglandin F<sub>2α</sub> were determined using immunofluorescence. No significant correlations were observed for F<sub>2</sub>-IsoP and RvD1 levels with the semen variables. Based on F<sub>2</sub>-IsoP levels, individuals were classified into two groups: Group 1 (F<sub>2</sub>-IsoPs ≤ 29.96 ng/mL, 51%) and Group 2 (F<sub>2</sub>-IsoPs > 29.96 ng/mL, 49%). In comparison to Group 1, Group 2 showed significantly higher F<sub>2</sub>-IsoP levels (13.33 ng/mL vs. 44.80 ng/mL; <i>p</i> < 0.05), a lower progressive motility percentage (30% vs. 25%; <i>p</i> < 0.05), and increased RvD1 levels (36.09% vs. 44.94%). Immunofluorescence analysis revealed a different localization of 8-IsoProstaglandin F<sub>2α</sub> in the ejaculated sperm of Group 1 compared to that observed in Group 2. A weak signal was detected in the sperm tail (Group 1, 79.1% vs. Group 2, 36.9; <i>p</i> < 0.01). In spermatozoa of Group 2 patients, a strong signal in the acrosome, midpiece, and tail was highlighted. These findings suggest the need to test oxidative stress during routine semen analysis in patients with idiopathic infertility to improve diagnosis and treatment. |
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| language | English |
| publishDate | 2025-04-01 |
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| spelling | doaj-art-f7d2a6b9d83d4abcbbf2eaee8ab106692025-08-20T03:14:17ZengMDPI AGBiology2079-77372025-04-0114445010.3390/biology14040450Seminal F<sub>2</sub>-IsoP and RvD1 Levels in Idiopathic Infertile MenElena Moretti0Giulia Collodel1Caterina Marcucci2Laura Liguori3Laura Gambera4Cinzia Signorini5Department of Molecular and Developmental Medicine, University of Siena, 53100 Siena, ItalyDepartment of Molecular and Developmental Medicine, University of Siena, 53100 Siena, ItalyDepartment of Molecular and Developmental Medicine, University of Siena, 53100 Siena, ItalyDepartment of Molecular and Developmental Medicine, University of Siena, 53100 Siena, ItalyFertility Center, AGI Medica, 53100 Siena, ItalyDepartment of Molecular and Developmental Medicine, University of Siena, 53100 Siena, Italy30 percent of infertile men are diagnosed with idiopathic infertility. This study aimed to assess oxidative stress in the semen of 77 patients with idiopathic infertility by measuring F<sub>2</sub>-isoprostane (F<sub>2</sub>-IsoP), resolvin D1 (RvD1) levels, and semen parameters. The presence and localization of 8-IsoProstaglandin F<sub>2α</sub> were determined using immunofluorescence. No significant correlations were observed for F<sub>2</sub>-IsoP and RvD1 levels with the semen variables. Based on F<sub>2</sub>-IsoP levels, individuals were classified into two groups: Group 1 (F<sub>2</sub>-IsoPs ≤ 29.96 ng/mL, 51%) and Group 2 (F<sub>2</sub>-IsoPs > 29.96 ng/mL, 49%). In comparison to Group 1, Group 2 showed significantly higher F<sub>2</sub>-IsoP levels (13.33 ng/mL vs. 44.80 ng/mL; <i>p</i> < 0.05), a lower progressive motility percentage (30% vs. 25%; <i>p</i> < 0.05), and increased RvD1 levels (36.09% vs. 44.94%). Immunofluorescence analysis revealed a different localization of 8-IsoProstaglandin F<sub>2α</sub> in the ejaculated sperm of Group 1 compared to that observed in Group 2. A weak signal was detected in the sperm tail (Group 1, 79.1% vs. Group 2, 36.9; <i>p</i> < 0.01). In spermatozoa of Group 2 patients, a strong signal in the acrosome, midpiece, and tail was highlighted. These findings suggest the need to test oxidative stress during routine semen analysis in patients with idiopathic infertility to improve diagnosis and treatment.https://www.mdpi.com/2079-7737/14/4/450F<sub>2</sub>-isoprostaneshuman semenidiopathic infertilityoxidative stressresolvin D1 |
| spellingShingle | Elena Moretti Giulia Collodel Caterina Marcucci Laura Liguori Laura Gambera Cinzia Signorini Seminal F<sub>2</sub>-IsoP and RvD1 Levels in Idiopathic Infertile Men Biology F<sub>2</sub>-isoprostanes human semen idiopathic infertility oxidative stress resolvin D1 |
| title | Seminal F<sub>2</sub>-IsoP and RvD1 Levels in Idiopathic Infertile Men |
| title_full | Seminal F<sub>2</sub>-IsoP and RvD1 Levels in Idiopathic Infertile Men |
| title_fullStr | Seminal F<sub>2</sub>-IsoP and RvD1 Levels in Idiopathic Infertile Men |
| title_full_unstemmed | Seminal F<sub>2</sub>-IsoP and RvD1 Levels in Idiopathic Infertile Men |
| title_short | Seminal F<sub>2</sub>-IsoP and RvD1 Levels in Idiopathic Infertile Men |
| title_sort | seminal f sub 2 sub isop and rvd1 levels in idiopathic infertile men |
| topic | F<sub>2</sub>-isoprostanes human semen idiopathic infertility oxidative stress resolvin D1 |
| url | https://www.mdpi.com/2079-7737/14/4/450 |
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