Seminal F<sub>2</sub>-IsoP and RvD1 Levels in Idiopathic Infertile Men

Seminal F<sub>2</sub>-IsoP and RvD1 Levels in Idiopathic Infertile Men

30 percent of infertile men are diagnosed with idiopathic infertility. This study aimed to assess oxidative stress in the semen of 77 patients with idiopathic infertility by measuring F<sub>2</sub>-isoprostane (F<sub>2</sub>-IsoP), resolvin D1 (RvD1) levels, and semen paramet...

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Bibliographic Details
Main Authors: Elena Moretti, Giulia Collodel, Caterina Marcucci, Laura Liguori, Laura Gambera, Cinzia Signorini
Format: Article
Language:English
Published: MDPI AG 2025-04-01
Series:Biology
Subjects:
F<sub>2</sub>-isoprostanes
human semen
idiopathic infertility
oxidative stress
resolvin D1
Online Access:https://www.mdpi.com/2079-7737/14/4/450
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Summary:30 percent of infertile men are diagnosed with idiopathic infertility. This study aimed to assess oxidative stress in the semen of 77 patients with idiopathic infertility by measuring F<sub>2</sub>-isoprostane (F<sub>2</sub>-IsoP), resolvin D1 (RvD1) levels, and semen parameters. The presence and localization of 8-IsoProstaglandin F<sub>2α</sub> were determined using immunofluorescence. No significant correlations were observed for F<sub>2</sub>-IsoP and RvD1 levels with the semen variables. Based on F<sub>2</sub>-IsoP levels, individuals were classified into two groups: Group 1 (F<sub>2</sub>-IsoPs ≤ 29.96 ng/mL, 51%) and Group 2 (F<sub>2</sub>-IsoPs > 29.96 ng/mL, 49%). In comparison to Group 1, Group 2 showed significantly higher F<sub>2</sub>-IsoP levels (13.33 ng/mL vs. 44.80 ng/mL; <i>p</i> < 0.05), a lower progressive motility percentage (30% vs. 25%; <i>p</i> < 0.05), and increased RvD1 levels (36.09% vs. 44.94%). Immunofluorescence analysis revealed a different localization of 8-IsoProstaglandin F<sub>2α</sub> in the ejaculated sperm of Group 1 compared to that observed in Group 2. A weak signal was detected in the sperm tail (Group 1, 79.1% vs. Group 2, 36.9; <i>p</i> < 0.01). In spermatozoa of Group 2 patients, a strong signal in the acrosome, midpiece, and tail was highlighted. These findings suggest the need to test oxidative stress during routine semen analysis in patients with idiopathic infertility to improve diagnosis and treatment.
ISSN:2079-7737

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