Informative EST-SSR markers for genotyping and intraspecific differentiation of Brassica oleracea var. capitata L.
Brassica oleraceae var. capitata L. is characterized by a high level of intraspecific heterogeneity due to some biological features that cause difficulties for breeding creating genetically homogenous forms and maintaining their genetic purity. Microsatellites (SSR) are highly polymorphic markers of...
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| Format: | Article |
| Language: | English |
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Siberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and Breeders
2016-03-01
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| Series: | Вавиловский журнал генетики и селекции |
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| Online Access: | https://vavilov.elpub.ru/jour/article/view/524 |
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| author | M. N. Shapturenko T. V. Pechkovskaya S. I. Vakula A. V. Jakimovich Yu. M. Zabara L. V. Khotyleva |
| author_facet | M. N. Shapturenko T. V. Pechkovskaya S. I. Vakula A. V. Jakimovich Yu. M. Zabara L. V. Khotyleva |
| author_sort | M. N. Shapturenko |
| collection | DOAJ |
| description | Brassica oleraceae var. capitata L. is characterized by a high level of intraspecific heterogeneity due to some biological features that cause difficulties for breeding creating genetically homogenous forms and maintaining their genetic purity. Microsatellites (SSR) are highly polymorphic markers of plant genomes and represent one of the most effective tools for assessing genetic polymorphism. Among microsatellites, EST-SSR are most interesting, because they are directly linked to the expressed sequences and for that reason are widely used for analysis of genetic diversity and population structure. In this work, we studied the effectiveness of the use of transferable EST-SSR markers for both analyzing white cabbage diversity and genotyping pure lines. As a result, 15 microsatellite loci were characterized for the information content, allelic frequencies and heterogeneity levels. The effective multiallelic markers (Bo20TR, BoDCTD4, BoPC34, BoPLD1, BoCalc, BoPC15) with high information content (PIC > 0.7) that could be successfully used for analysis of inter- and intravarietal polymorphism in B. oleracea var. capitata were identified. It has been shown that intervarietal polymorphism expressed as the allelic diversity of EST SSR loci greatly facilitates varietal identification and typing of individual plants for breeding purposes. Based on the SSR-evaluation and subsequent clustering, the genetic structure of the breeding collection was identified, which showed that most experimental forms, in spite of different origin, have a common ancestral genetic basis. The identified donors of rare alleles could potentially be a source of valuable genetic segregation for further B. oleracea breeding improvement. |
| format | Article |
| id | doaj-art-f7b76cbdd6c04d2d871b667afc409e7a |
| institution | Kabale University |
| issn | 2500-3259 |
| language | English |
| publishDate | 2016-03-01 |
| publisher | Siberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and Breeders |
| record_format | Article |
| series | Вавиловский журнал генетики и селекции |
| spelling | doaj-art-f7b76cbdd6c04d2d871b667afc409e7a2025-02-01T09:58:02ZengSiberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and BreedersВавиловский журнал генетики и селекции2500-32592016-03-012015156445Informative EST-SSR markers for genotyping and intraspecific differentiation of Brassica oleracea var. capitata L.M. N. Shapturenko0T. V. Pechkovskaya1S. I. Vakula2A. V. Jakimovich3Yu. M. Zabara4L. V. Khotyleva5Institute of Genetics and Cytology of NAS, Minsk, BelarusInstitute of Genetics and Cytology of NAS, Minsk, BelarusInstitute of Genetics and Cytology of NAS, Minsk, BelarusInstitute of Vegetable Growing, Minsk oblast, Minsk region, Samokhvalovichy, BelarusInstitute of Vegetable Growing, Minsk oblast, Minsk region, Samokhvalovichy, BelarusInstitute of Genetics and Cytology of NAS, Minsk, BelarusBrassica oleraceae var. capitata L. is characterized by a high level of intraspecific heterogeneity due to some biological features that cause difficulties for breeding creating genetically homogenous forms and maintaining their genetic purity. Microsatellites (SSR) are highly polymorphic markers of plant genomes and represent one of the most effective tools for assessing genetic polymorphism. Among microsatellites, EST-SSR are most interesting, because they are directly linked to the expressed sequences and for that reason are widely used for analysis of genetic diversity and population structure. In this work, we studied the effectiveness of the use of transferable EST-SSR markers for both analyzing white cabbage diversity and genotyping pure lines. As a result, 15 microsatellite loci were characterized for the information content, allelic frequencies and heterogeneity levels. The effective multiallelic markers (Bo20TR, BoDCTD4, BoPC34, BoPLD1, BoCalc, BoPC15) with high information content (PIC > 0.7) that could be successfully used for analysis of inter- and intravarietal polymorphism in B. oleracea var. capitata were identified. It has been shown that intervarietal polymorphism expressed as the allelic diversity of EST SSR loci greatly facilitates varietal identification and typing of individual plants for breeding purposes. Based on the SSR-evaluation and subsequent clustering, the genetic structure of the breeding collection was identified, which showed that most experimental forms, in spite of different origin, have a common ancestral genetic basis. The identified donors of rare alleles could potentially be a source of valuable genetic segregation for further B. oleracea breeding improvement.https://vavilov.elpub.ru/jour/article/view/524brassica oleracea var. capitata l.est-ssr markersgenetic polymorphisms |
| spellingShingle | M. N. Shapturenko T. V. Pechkovskaya S. I. Vakula A. V. Jakimovich Yu. M. Zabara L. V. Khotyleva Informative EST-SSR markers for genotyping and intraspecific differentiation of Brassica oleracea var. capitata L. Вавиловский журнал генетики и селекции brassica oleracea var. capitata l. est-ssr markers genetic polymorphisms |
| title | Informative EST-SSR markers for genotyping and intraspecific differentiation of Brassica oleracea var. capitata L. |
| title_full | Informative EST-SSR markers for genotyping and intraspecific differentiation of Brassica oleracea var. capitata L. |
| title_fullStr | Informative EST-SSR markers for genotyping and intraspecific differentiation of Brassica oleracea var. capitata L. |
| title_full_unstemmed | Informative EST-SSR markers for genotyping and intraspecific differentiation of Brassica oleracea var. capitata L. |
| title_short | Informative EST-SSR markers for genotyping and intraspecific differentiation of Brassica oleracea var. capitata L. |
| title_sort | informative est ssr markers for genotyping and intraspecific differentiation of brassica oleracea var capitata l |
| topic | brassica oleracea var. capitata l. est-ssr markers genetic polymorphisms |
| url | https://vavilov.elpub.ru/jour/article/view/524 |
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