Behavioral phenotyping of mice deficient for tumor necrosis factor

The tumor necrosis factor (TNF) is a cytokine exerting both homeostatic and pathophysiological roles in the central nervous system (CNS). It has been demonstrated that TNF plays roles in such diseases as Alzheimer’s disease, Parkinson’s disease and multiple sclerosis. Nevertheless, the role of TNF i...

Full description

Saved in:
Bibliographic Details
Main Authors: D. V. Fursenko, N. V. Khotskin, V. A. Kulikov, A. V. Kulikov
Format: Article
Language:English
Published: Siberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and Breeders 2015-12-01
Series:Вавиловский журнал генетики и селекции
Subjects:
Online Access:https://vavilov.elpub.ru/jour/article/view/424
Tags: Add Tag
No Tags, Be the first to tag this record!
_version_ 1832575268932288512
author D. V. Fursenko
N. V. Khotskin
V. A. Kulikov
A. V. Kulikov
author_facet D. V. Fursenko
N. V. Khotskin
V. A. Kulikov
A. V. Kulikov
author_sort D. V. Fursenko
collection DOAJ
description The tumor necrosis factor (TNF) is a cytokine exerting both homeostatic and pathophysiological roles in the central nervous system (CNS). It has been demonstrated that TNF plays roles in such diseases as Alzheimer’s disease, Parkinson’s disease and multiple sclerosis. Nevertheless, the role of TNF in the CNS under normal physiological conditions is poorly studied. A novel mouse strain with TNF deficiency (TNFKO) was developed in S.A. Nedospa­sov’s labora­tory of Engelhardt Institute of Molecular Bio­logy of the Russian Academy of Sciences on the C57Bl/6 (WT) background. In our study, we compared the behavior of TNFKO and WT adult mice in a battery of tests: open-field, elevated plus-maze and the forced-swim test. We showed that TNF deficiency had no effect on locomotor activity or exploration in the openfield test. At the same time, in this test, TNFKO mice spent more time in the center of the arena, but had a higher level of defecation and lower rearing duration. This result indicates that, in the openfield conditions, TNFKO mice show disorientation rather than anxiety-like behavior. There were no differences between TNFKO and WT in anxiety level in the elevated plus-maze test or in depressive-like behavior in the forcedswim test. These data suggest that TNF deficiency leads to changes in neurofunctional interactions that alter the mouse response to mild stress in the open-field test.
format Article
id doaj-art-f78f02c6185140c6b28e11f1e89e83eb
institution Kabale University
issn 2500-3259
language English
publishDate 2015-12-01
publisher Siberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and Breeders
record_format Article
series Вавиловский журнал генетики и селекции
spelling doaj-art-f78f02c6185140c6b28e11f1e89e83eb2025-02-01T09:58:02ZengSiberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and BreedersВавиловский журнал генетики и селекции2500-32592015-12-0119439439810.18699/VJ15.050383Behavioral phenotyping of mice deficient for tumor necrosis factorD. V. Fursenko0N. V. Khotskin1V. A. Kulikov2A. V. Kulikov3Institute of Cytology and Genetics SB RAS, Novosibirsk, RussiaInstitute of Cytology and Genetics SB RAS, Novosibirsk, RussiaInstitute of Automation and Electrometry SB RAS, Novosibirsk, RussiaInstitute of Cytology and Genetics SB RAS, Novosibirsk, RussiaThe tumor necrosis factor (TNF) is a cytokine exerting both homeostatic and pathophysiological roles in the central nervous system (CNS). It has been demonstrated that TNF plays roles in such diseases as Alzheimer’s disease, Parkinson’s disease and multiple sclerosis. Nevertheless, the role of TNF in the CNS under normal physiological conditions is poorly studied. A novel mouse strain with TNF deficiency (TNFKO) was developed in S.A. Nedospa­sov’s labora­tory of Engelhardt Institute of Molecular Bio­logy of the Russian Academy of Sciences on the C57Bl/6 (WT) background. In our study, we compared the behavior of TNFKO and WT adult mice in a battery of tests: open-field, elevated plus-maze and the forced-swim test. We showed that TNF deficiency had no effect on locomotor activity or exploration in the openfield test. At the same time, in this test, TNFKO mice spent more time in the center of the arena, but had a higher level of defecation and lower rearing duration. This result indicates that, in the openfield conditions, TNFKO mice show disorientation rather than anxiety-like behavior. There were no differences between TNFKO and WT in anxiety level in the elevated plus-maze test or in depressive-like behavior in the forcedswim test. These data suggest that TNF deficiency leads to changes in neurofunctional interactions that alter the mouse response to mild stress in the open-field test.https://vavilov.elpub.ru/jour/article/view/424tumor necrosis factorknockout miceopenfieldelevated plus-mazeforced-swim test
spellingShingle D. V. Fursenko
N. V. Khotskin
V. A. Kulikov
A. V. Kulikov
Behavioral phenotyping of mice deficient for tumor necrosis factor
Вавиловский журнал генетики и селекции
tumor necrosis factor
knockout mice
openfield
elevated plus-maze
forced-swim test
title Behavioral phenotyping of mice deficient for tumor necrosis factor
title_full Behavioral phenotyping of mice deficient for tumor necrosis factor
title_fullStr Behavioral phenotyping of mice deficient for tumor necrosis factor
title_full_unstemmed Behavioral phenotyping of mice deficient for tumor necrosis factor
title_short Behavioral phenotyping of mice deficient for tumor necrosis factor
title_sort behavioral phenotyping of mice deficient for tumor necrosis factor
topic tumor necrosis factor
knockout mice
openfield
elevated plus-maze
forced-swim test
url https://vavilov.elpub.ru/jour/article/view/424
work_keys_str_mv AT dvfursenko behavioralphenotypingofmicedeficientfortumornecrosisfactor
AT nvkhotskin behavioralphenotypingofmicedeficientfortumornecrosisfactor
AT vakulikov behavioralphenotypingofmicedeficientfortumornecrosisfactor
AT avkulikov behavioralphenotypingofmicedeficientfortumornecrosisfactor