Kajian patogenisitas bakteri Edwardsiella ictaluri pada ikan patin Pangasionodon hypophthalmus
ABSTRACT One of major problem of striped catfish Pangasionodon hypophthalmus culture is enteric septicemia of catfish (ESC), bacterial disease of Edwardsiella ictaluri, caused of more than 50% of mortalities. This reaserch was aimed to determine pathogenicity of local isolate E. ictaluri. Thirty i...
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Asosiasi Sains Akuakultur Indonesia
2016-12-01
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| Series: | Jurnal Akuakultur Indonesia |
| Online Access: | https://journal.ipb.ac.id/index.php/jai/article/view/12603 |
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| author | Wiwik Susanti Agustin Indrawati Fachriyan H Pasaribu |
| author_facet | Wiwik Susanti Agustin Indrawati Fachriyan H Pasaribu |
| author_sort | Wiwik Susanti |
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| description | ABSTRACT
One of major problem of striped catfish Pangasionodon hypophthalmus culture is enteric septicemia of catfish (ESC), bacterial disease of Edwardsiella ictaluri, caused of more than 50% of mortalities. This reaserch was aimed to determine pathogenicity of local isolate E. ictaluri. Thirty individu of five group fishes, 6–10 g in body weight, injected intraperitoneally with 0,1 mL of bacteria suspension of 102 cfu/mL; 104 cfu/mL; 106 cfu/mL; 108 cfu/mL; 1010 cfu/mL; and PBS as control, were culture in 18 of 60×40×45 cm3 aquarium for seven days. External organs of fish (skin and abdomen) and internal organs (liver, kidney, and brain) were examined macroscopicly and microscopicly. Internal organ sample were taken on the 5th day for histopatologic test while blood sample was on the 1st, 3rd, and 5th day after infection. Mortality rate was count to reach LD50. Clinical signs and pathology anatomy of co-infection fish showed vertical swim, petechial hemorrhage in the skin, dropsy, ascites in the abdominal cavity, pale liver and the kidney was dark red. Histopathology showed hydropic degeneration, fatty degeneration, hemorrhage and necrosis in the liver, melano macrophage center (MMC) and necrosis in the kidneys, hemorrhage, and inflammatory cell infiltrates were also found in the kidneys and brain. Decreased of hematocrit and hemoglobin values of all tread group were statistically significant different (P<0,05) compared to controls. LD50 dose was 2,8×104 cfu/mL. The result indicated that E. ictaluri was very pathogenic on striped catfish P. hypophthalmus.
Keywords: Edwardsiella ictaluri, enteric septicemia of catfish (ESC), pathogenicity, striped catfish
ABSTRAK
Salah satu kendala yang dijumpai pada budidaya ikan patin Pangasionodon hypophthalmus yaitu serangan penyakit bakterial. Enteric septicemia of catfish (ESC) adalah penyakit infeksi bakteri Edwardsiella ictaluri yang dapat menyebabkan kematian ikan patin sampai >50%. Penelitian ini dilakukan untuk mengetahui patogenisitas E. ictaluri isolat lokal pada ikan patin. Masing-masing 30 ekor ikan patin ukuran 6–10 g/ekor diinjeksi secara intraperitoneal dengan 0,1 mL larutan bakteri kepadatan 102 cfu/mL; 104 cfu/mL; 106 cfu/mL; 108 cfu/mL; 1010 cfu/mL; dan PBS sebagai kontrol. Ikan dipelihara selama tujuh hari pada akuarium berukuran 60×40×45 cm3. Pemeriksaan makroskopis dan mikroskopis dilakukan terhadap organ eksternal (kulit dan abdomen) dan internal (hati, ginjal, dan otak). Pengambilan sampel organ internal untuk uji histopatologi pada hari kelima dan sampel darah untuk uji gambaran darah pada hari pertama, ketiga, dan kelima pascainfeksi. Jumlah kematian ikan dihitung untuk mendapat nilai LD50. Pengamatan gejala klinis dan patologi anatomi ditemukan ikan berenang vertikal, adanya bercak merah pada kulit, pembengkakan abdomen, asites, hati pucat, dan ginjal berwarna merah kehitaman. Hasil histopatologi terlihat terjadinya degenerasi hidropik, degenerasi lemak, melano macrophage center (MMC), nekrosa, hemoragi, dan infiltrasi sel radang pada hati, ginjal, dan otak. Penurunan nilai hematokrit dan hemoglobin pada perlakuan secara statistik berbeda nyata (P<0,05) dengan kontrol. Dosis LD50 didapat 2,8×104 cfu/mL. Hasil penelitian ini mengindikasikan bahwa E. ictaluri pada ikan patin bersifat sangat patogen.
Kata kunci: Edwardsiella ictaluri, enteric septicemia of catfish (ESC), patogenisitas, patin
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| format | Article |
| id | doaj-art-f78ba111066a495b82c6c4f774f53579 |
| institution | Kabale University |
| issn | 1412-5269 2354-6700 |
| language | English |
| publishDate | 2016-12-01 |
| publisher | Asosiasi Sains Akuakultur Indonesia |
| record_format | Article |
| series | Jurnal Akuakultur Indonesia |
| spelling | doaj-art-f78ba111066a495b82c6c4f774f535792025-01-24T17:58:21ZengAsosiasi Sains Akuakultur IndonesiaJurnal Akuakultur Indonesia1412-52692354-67002016-12-0115210.19027/jai.15.99-1079934Kajian patogenisitas bakteri Edwardsiella ictaluri pada ikan patin Pangasionodon hypophthalmusWiwik Susanti0Agustin Indrawati1Fachriyan H Pasaribu2Balai Perikanan Budidaya Air Tawar Mandiangin Kalimantan SelatanProgram Studi Mikrobiologi Medik, Fakultas Kedokteran Hewan, Institut Pertanian BogorProgram Studi Mikrobiologi Medik, Fakultas Kedokteran Hewan, Institut Pertanian BogorABSTRACT One of major problem of striped catfish Pangasionodon hypophthalmus culture is enteric septicemia of catfish (ESC), bacterial disease of Edwardsiella ictaluri, caused of more than 50% of mortalities. This reaserch was aimed to determine pathogenicity of local isolate E. ictaluri. Thirty individu of five group fishes, 6–10 g in body weight, injected intraperitoneally with 0,1 mL of bacteria suspension of 102 cfu/mL; 104 cfu/mL; 106 cfu/mL; 108 cfu/mL; 1010 cfu/mL; and PBS as control, were culture in 18 of 60×40×45 cm3 aquarium for seven days. External organs of fish (skin and abdomen) and internal organs (liver, kidney, and brain) were examined macroscopicly and microscopicly. Internal organ sample were taken on the 5th day for histopatologic test while blood sample was on the 1st, 3rd, and 5th day after infection. Mortality rate was count to reach LD50. Clinical signs and pathology anatomy of co-infection fish showed vertical swim, petechial hemorrhage in the skin, dropsy, ascites in the abdominal cavity, pale liver and the kidney was dark red. Histopathology showed hydropic degeneration, fatty degeneration, hemorrhage and necrosis in the liver, melano macrophage center (MMC) and necrosis in the kidneys, hemorrhage, and inflammatory cell infiltrates were also found in the kidneys and brain. Decreased of hematocrit and hemoglobin values of all tread group were statistically significant different (P<0,05) compared to controls. LD50 dose was 2,8×104 cfu/mL. The result indicated that E. ictaluri was very pathogenic on striped catfish P. hypophthalmus. Keywords: Edwardsiella ictaluri, enteric septicemia of catfish (ESC), pathogenicity, striped catfish ABSTRAK Salah satu kendala yang dijumpai pada budidaya ikan patin Pangasionodon hypophthalmus yaitu serangan penyakit bakterial. Enteric septicemia of catfish (ESC) adalah penyakit infeksi bakteri Edwardsiella ictaluri yang dapat menyebabkan kematian ikan patin sampai >50%. Penelitian ini dilakukan untuk mengetahui patogenisitas E. ictaluri isolat lokal pada ikan patin. Masing-masing 30 ekor ikan patin ukuran 6–10 g/ekor diinjeksi secara intraperitoneal dengan 0,1 mL larutan bakteri kepadatan 102 cfu/mL; 104 cfu/mL; 106 cfu/mL; 108 cfu/mL; 1010 cfu/mL; dan PBS sebagai kontrol. Ikan dipelihara selama tujuh hari pada akuarium berukuran 60×40×45 cm3. Pemeriksaan makroskopis dan mikroskopis dilakukan terhadap organ eksternal (kulit dan abdomen) dan internal (hati, ginjal, dan otak). Pengambilan sampel organ internal untuk uji histopatologi pada hari kelima dan sampel darah untuk uji gambaran darah pada hari pertama, ketiga, dan kelima pascainfeksi. Jumlah kematian ikan dihitung untuk mendapat nilai LD50. Pengamatan gejala klinis dan patologi anatomi ditemukan ikan berenang vertikal, adanya bercak merah pada kulit, pembengkakan abdomen, asites, hati pucat, dan ginjal berwarna merah kehitaman. Hasil histopatologi terlihat terjadinya degenerasi hidropik, degenerasi lemak, melano macrophage center (MMC), nekrosa, hemoragi, dan infiltrasi sel radang pada hati, ginjal, dan otak. Penurunan nilai hematokrit dan hemoglobin pada perlakuan secara statistik berbeda nyata (P<0,05) dengan kontrol. Dosis LD50 didapat 2,8×104 cfu/mL. Hasil penelitian ini mengindikasikan bahwa E. ictaluri pada ikan patin bersifat sangat patogen. Kata kunci: Edwardsiella ictaluri, enteric septicemia of catfish (ESC), patogenisitas, patin https://journal.ipb.ac.id/index.php/jai/article/view/12603 |
| spellingShingle | Wiwik Susanti Agustin Indrawati Fachriyan H Pasaribu Kajian patogenisitas bakteri Edwardsiella ictaluri pada ikan patin Pangasionodon hypophthalmus Jurnal Akuakultur Indonesia |
| title | Kajian patogenisitas bakteri Edwardsiella ictaluri pada ikan patin Pangasionodon hypophthalmus |
| title_full | Kajian patogenisitas bakteri Edwardsiella ictaluri pada ikan patin Pangasionodon hypophthalmus |
| title_fullStr | Kajian patogenisitas bakteri Edwardsiella ictaluri pada ikan patin Pangasionodon hypophthalmus |
| title_full_unstemmed | Kajian patogenisitas bakteri Edwardsiella ictaluri pada ikan patin Pangasionodon hypophthalmus |
| title_short | Kajian patogenisitas bakteri Edwardsiella ictaluri pada ikan patin Pangasionodon hypophthalmus |
| title_sort | kajian patogenisitas bakteri edwardsiella ictaluri pada ikan patin pangasionodon hypophthalmus |
| url | https://journal.ipb.ac.id/index.php/jai/article/view/12603 |
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