Knockdown of PRR14 inhibits the growth and migration of gastric cancer cells
Background: Although proline-rich protein (PRR) 14 has been implicated in various malignancies, its involvement in stomach cancer is yet unclear. In order to improve therapeutic approaches for gastric cancer, this study intends to explore the ways in which PRR14 controls the growth and metastasis...
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| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
MRE Press
2025-06-01
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| Series: | Journal of Men's Health |
| Subjects: | |
| Online Access: | https://oss.jomh.org/files/article/20250627-578/pdf/JOMH2025042801.pdf |
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| Summary: | Background: Although proline-rich protein (PRR) 14 has been implicated
in various malignancies, its involvement in stomach cancer is yet unclear. In
order to improve therapeutic approaches for gastric cancer, this study intends to
explore the ways in which PRR14 controls the growth and metastasis of gastric
cancer cells. Methods: PRR14 expression levels in gastric cancer were
analyzed using the The University of Alabama at
Birmingham CANcer data analysis Portal (UALCAN) database, while the Kaplan-Meier
Plotter database was used to evaluate the impact of PRR14 expression on overall
survival in gastric cancer patients. After PRR14 knockdown, cell proliferation
was evaluated using colony formation and cell counting kit-8 (CCK8) assays. Using
flow cytometry, the rates of cell apoptosis were determined. Cell migration and
invasion were assessed using transwell and wound healing assays. Protein
expression in the phosphatidylinositol 3-kinase (PI3K)/protein kinase B (Akt)
signaling pathway was evaluated by Western blotting.
Results: Significant expression of
PRR14 was discovered in stomach cancer tissues, and it was associated with poor
prognosis. Knockdown of PRR14 significantly reduced cell viability, colony
formation, migration and invasion, while promoting apoptosis in gastric cancer
cells. Furthermore, the expression levels of p-PI3K and
p-Akt were markedly decreased following PRR14
knockdown. Conclusions: PRR14 knockdown inhibits the proliferation,
migration and invasion of gastric cancer cells while promoting apoptosis,
possibly via blocking the PI3K/Akt signaling pathway. According to these results,
PRR14 might be a viable target for treatment in gastric cancer. |
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| ISSN: | 1875-6867 1875-6859 |