Culture Medium Enriched with Ultrafine Carbon Monoxide Bubbles Enhances In Vitro Blastocyst Formation of In Vivo-Fertilized Mouse Zygotes
Oxidative stress induced by in vitro culture conditions impedes the differentiation of fertilized zygotes. Gasotransmitters containing carbon monoxide (CO) exhibit antioxidant properties when exogenously administered at appropriate concentrations. In this study, CO was incorporated into ultrafine bu...
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MDPI AG
2025-06-01
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| Series: | Antioxidants |
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| Online Access: | https://www.mdpi.com/2076-3921/14/6/684 |
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| author | Toyofumi Hirakawa Kazuhiko Nakabayashi Noriko Ito Keisuke Ishiwata Daichi Urushiyama Kohei Miyata Tsukasa Baba Kenichiro Hata Shin’ichiro Yasunaga Fusanori Yotsumoto Katsuro Tachibana Shingo Miyamoto |
| author_facet | Toyofumi Hirakawa Kazuhiko Nakabayashi Noriko Ito Keisuke Ishiwata Daichi Urushiyama Kohei Miyata Tsukasa Baba Kenichiro Hata Shin’ichiro Yasunaga Fusanori Yotsumoto Katsuro Tachibana Shingo Miyamoto |
| author_sort | Toyofumi Hirakawa |
| collection | DOAJ |
| description | Oxidative stress induced by in vitro culture conditions impedes the differentiation of fertilized zygotes. Gasotransmitters containing carbon monoxide (CO) exhibit antioxidant properties when exogenously administered at appropriate concentrations. In this study, CO was incorporated into ultrafine bubbles (UFBs) to devise an innovative method for promoting the efficient differentiation of fertilized mouse zygotes into blastocysts within an in vitro culture environment. While CO typically dissipates rapidly in culture media, its encapsulation into UFBs enabled its prolonged retention within the medium. Fertilized mouse zygotes cultured in the UFB medium exhibited a significantly higher rate of blastocyst hatching compared to those cultured in conventional media. Furthermore, Gene Ontology analysis revealed elevated expression of mitochondrial-related genes and genes essential for blastocyst maturation in the UFB culture medium. These findings underscore the potential of CO-UFB as a potent agent for improving in vitro blastocyst formation and hatching by mitigating oxidative stress, thereby offering a promising strategy for enhancing assisted reproductive technologies. |
| format | Article |
| id | doaj-art-f752115e71cc4e53b5f7d092b6fcdc1a |
| institution | OA Journals |
| issn | 2076-3921 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antioxidants |
| spelling | doaj-art-f752115e71cc4e53b5f7d092b6fcdc1a2025-08-20T02:24:39ZengMDPI AGAntioxidants2076-39212025-06-0114668410.3390/antiox14060684Culture Medium Enriched with Ultrafine Carbon Monoxide Bubbles Enhances In Vitro Blastocyst Formation of In Vivo-Fertilized Mouse ZygotesToyofumi Hirakawa0Kazuhiko Nakabayashi1Noriko Ito2Keisuke Ishiwata3Daichi Urushiyama4Kohei Miyata5Tsukasa Baba6Kenichiro Hata7Shin’ichiro Yasunaga8Fusanori Yotsumoto9Katsuro Tachibana10Shingo Miyamoto11Department of Obstetrics & Gynecology, Faculty of Medicine, Fukuoka University, Fukuoka 8140180, JapanDepartment of Maternal-Fetal Biology, National Center for Child Health and Development, Tokyo 1578535, JapanDepartment of Maternal-Fetal Biology, National Center for Child Health and Development, Tokyo 1578535, JapanDepartment of Maternal-Fetal Biology, National Center for Child Health and Development, Tokyo 1578535, JapanDepartment of Obstetrics & Gynecology, Faculty of Medicine, Fukuoka University, Fukuoka 8140180, JapanDepartment of Obstetrics & Gynecology, Faculty of Medicine, Fukuoka University, Fukuoka 8140180, JapanDepartment of Obstetrics & Gynecology, School of Medicine, Iwate Medical University, Morioka 0283695, JapanDepartment of Maternal-Fetal Biology, National Center for Child Health and Development, Tokyo 1578535, JapanDepartment of Biochemistry, Faculty of Medicine, Fukuoka University, Fukuoka 8140180, JapanDepartment of Obstetrics & Gynecology, Faculty of Medicine, Fukuoka University, Fukuoka 8140180, JapanDepartment of Anatomy, Faculty of Medicine, Fukuoka University, Fukuoka 8140180, JapanDepartment of Obstetrics & Gynecology, School of Medicine, Iwate Medical University, Morioka 0283695, JapanOxidative stress induced by in vitro culture conditions impedes the differentiation of fertilized zygotes. Gasotransmitters containing carbon monoxide (CO) exhibit antioxidant properties when exogenously administered at appropriate concentrations. In this study, CO was incorporated into ultrafine bubbles (UFBs) to devise an innovative method for promoting the efficient differentiation of fertilized mouse zygotes into blastocysts within an in vitro culture environment. While CO typically dissipates rapidly in culture media, its encapsulation into UFBs enabled its prolonged retention within the medium. Fertilized mouse zygotes cultured in the UFB medium exhibited a significantly higher rate of blastocyst hatching compared to those cultured in conventional media. Furthermore, Gene Ontology analysis revealed elevated expression of mitochondrial-related genes and genes essential for blastocyst maturation in the UFB culture medium. These findings underscore the potential of CO-UFB as a potent agent for improving in vitro blastocyst formation and hatching by mitigating oxidative stress, thereby offering a promising strategy for enhancing assisted reproductive technologies.https://www.mdpi.com/2076-3921/14/6/684gasotransmittercarbon monoxideultrafine bubbleblastocyst |
| spellingShingle | Toyofumi Hirakawa Kazuhiko Nakabayashi Noriko Ito Keisuke Ishiwata Daichi Urushiyama Kohei Miyata Tsukasa Baba Kenichiro Hata Shin’ichiro Yasunaga Fusanori Yotsumoto Katsuro Tachibana Shingo Miyamoto Culture Medium Enriched with Ultrafine Carbon Monoxide Bubbles Enhances In Vitro Blastocyst Formation of In Vivo-Fertilized Mouse Zygotes Antioxidants gasotransmitter carbon monoxide ultrafine bubble blastocyst |
| title | Culture Medium Enriched with Ultrafine Carbon Monoxide Bubbles Enhances In Vitro Blastocyst Formation of In Vivo-Fertilized Mouse Zygotes |
| title_full | Culture Medium Enriched with Ultrafine Carbon Monoxide Bubbles Enhances In Vitro Blastocyst Formation of In Vivo-Fertilized Mouse Zygotes |
| title_fullStr | Culture Medium Enriched with Ultrafine Carbon Monoxide Bubbles Enhances In Vitro Blastocyst Formation of In Vivo-Fertilized Mouse Zygotes |
| title_full_unstemmed | Culture Medium Enriched with Ultrafine Carbon Monoxide Bubbles Enhances In Vitro Blastocyst Formation of In Vivo-Fertilized Mouse Zygotes |
| title_short | Culture Medium Enriched with Ultrafine Carbon Monoxide Bubbles Enhances In Vitro Blastocyst Formation of In Vivo-Fertilized Mouse Zygotes |
| title_sort | culture medium enriched with ultrafine carbon monoxide bubbles enhances in vitro blastocyst formation of in vivo fertilized mouse zygotes |
| topic | gasotransmitter carbon monoxide ultrafine bubble blastocyst |
| url | https://www.mdpi.com/2076-3921/14/6/684 |
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