Decreased CD127 Expression on CD4+ T-Cells and Elevated Frequencies of CD4+CD25+CD127− T-Cells in Children with Long-Lasting Type 1 Diabetes
Pathobiology of type 1 diabetes (T1D) is predominantly associated with T-cell-related actions. Homeostasis of majority of T-cells is critically dependent on signals mediated by CD127 (interleukin-7 receptor, IL-7R). In contrast, regulatory T-cells express very little CD127 and thereby may be delinea...
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| Format: | Article |
| Language: | English |
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Wiley
2013-01-01
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| Series: | Clinical and Developmental Immunology |
| Online Access: | http://dx.doi.org/10.1155/2013/459210 |
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| author | Marcin Moniuszko Barbara Glowinska-Olszewska Malgorzata Rusak Marta Jeznach Kamil Grubczak Danuta Lipinska Robert Milewski Anna Justyna Milewska Milena Dabrowska Ewa Jablonska Adam Kretowski Maria Gorska Anna Bodzenta-Lukaszyk Artur Bossowski |
| author_facet | Marcin Moniuszko Barbara Glowinska-Olszewska Malgorzata Rusak Marta Jeznach Kamil Grubczak Danuta Lipinska Robert Milewski Anna Justyna Milewska Milena Dabrowska Ewa Jablonska Adam Kretowski Maria Gorska Anna Bodzenta-Lukaszyk Artur Bossowski |
| author_sort | Marcin Moniuszko |
| collection | DOAJ |
| description | Pathobiology of type 1 diabetes (T1D) is predominantly associated with T-cell-related actions. Homeostasis of majority of T-cells is critically dependent on signals mediated by CD127 (interleukin-7 receptor, IL-7R). In contrast, regulatory T-cells express very little CD127 and thereby may be delineated by CD4+CD25+CD127− phenotype. Here we aimed to analyze CD127 expression on CD4+ and CD8+ T-cells and enumerate CD4+CD25+CD127− T-cells in long-lasting T1D. T-cells were analyzed by flow cytometry and immunologic data were correlated with vascular, metabolic, and inflammatory parameters. We demonstrated significantly decreased CD127 levels on CD4+, but not CD8+, T cells in T1D pediatric patients. Interestingly, frequencies of CD4+CD25+CD127− T-cells were significantly enhanced in T1D children and correlated well with frequencies of CD34+CD144+ endothelial progenitor cells and CD4+CD25− T-cells. Levels of CD127 on both CD4+ and CD8+ T-cells in T1D patients were not correlated to each other or HbA1C. Interestingly, however, CD127 levels on CD4+ T-cells were significantly correlated to frequencies of CD4+CD25+CD127− T-cells, whereas CD127 levels on CD8+ T-cells were significantly correlated to concentrations of VEGF and triglycerides. Our data indicate that CD127 expression is differentially modulated on CD4+ and CD8+ T-cells in the course of T1D. Moreover, we demonstrated that, in contrast to recent-onset T1D, long-lasting T1D is associated with enhancement of T-cells with regulatory phenotype. |
| format | Article |
| id | doaj-art-f74ca6925ceb41c2acf1cea1e9ae7dbe |
| institution | Kabale University |
| issn | 1740-2522 1740-2530 |
| language | English |
| publishDate | 2013-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Clinical and Developmental Immunology |
| spelling | doaj-art-f74ca6925ceb41c2acf1cea1e9ae7dbe2025-08-20T03:25:53ZengWileyClinical and Developmental Immunology1740-25221740-25302013-01-01201310.1155/2013/459210459210Decreased CD127 Expression on CD4+ T-Cells and Elevated Frequencies of CD4+CD25+CD127− T-Cells in Children with Long-Lasting Type 1 DiabetesMarcin Moniuszko0Barbara Glowinska-Olszewska1Malgorzata Rusak2Marta Jeznach3Kamil Grubczak4Danuta Lipinska5Robert Milewski6Anna Justyna Milewska7Milena Dabrowska8Ewa Jablonska9Adam Kretowski10Maria Gorska11Anna Bodzenta-Lukaszyk12Artur Bossowski13Department of Regenerative Medicine and Immune Regulation, Medical University of Bialystok, 15-269 Bialystok, PolandDepartment of Pediatrics, Endocrinology, Diabetology with Cardiology Division, Medical University of Bialystok, 15-274 Bialystok, PolandDepartment of Hematological Diagnostics, Medical University of Bialystok, 15-274 Bialystok, PolandDepartment of Allergology and Internal Medicine, University of Bialystok, 15-276 Bialystok, PolandDepartment of Immunology, Medical University of Bialystok, 15-276 Bialystok, PolandDepartment of Endocrinology, Diabetology and Internal Medicine, Medical University of Bialystok, 15-276 Bialystok, PolandDepartment of Statistics and Medical Informatics, Medical University of Bialystok, 15-295 Bialystok, PolandDepartment of Statistics and Medical Informatics, Medical University of Bialystok, 15-295 Bialystok, PolandDepartment of Hematological Diagnostics, Medical University of Bialystok, 15-274 Bialystok, PolandDepartment of Immunology, Medical University of Bialystok, 15-276 Bialystok, PolandDepartment of Endocrinology, Diabetology and Internal Medicine, Medical University of Bialystok, 15-276 Bialystok, PolandDepartment of Endocrinology, Diabetology and Internal Medicine, Medical University of Bialystok, 15-276 Bialystok, PolandDepartment of Allergology and Internal Medicine, University of Bialystok, 15-276 Bialystok, PolandDepartment of Pediatrics, Endocrinology, Diabetology with Cardiology Division, Medical University of Bialystok, 15-274 Bialystok, PolandPathobiology of type 1 diabetes (T1D) is predominantly associated with T-cell-related actions. Homeostasis of majority of T-cells is critically dependent on signals mediated by CD127 (interleukin-7 receptor, IL-7R). In contrast, regulatory T-cells express very little CD127 and thereby may be delineated by CD4+CD25+CD127− phenotype. Here we aimed to analyze CD127 expression on CD4+ and CD8+ T-cells and enumerate CD4+CD25+CD127− T-cells in long-lasting T1D. T-cells were analyzed by flow cytometry and immunologic data were correlated with vascular, metabolic, and inflammatory parameters. We demonstrated significantly decreased CD127 levels on CD4+, but not CD8+, T cells in T1D pediatric patients. Interestingly, frequencies of CD4+CD25+CD127− T-cells were significantly enhanced in T1D children and correlated well with frequencies of CD34+CD144+ endothelial progenitor cells and CD4+CD25− T-cells. Levels of CD127 on both CD4+ and CD8+ T-cells in T1D patients were not correlated to each other or HbA1C. Interestingly, however, CD127 levels on CD4+ T-cells were significantly correlated to frequencies of CD4+CD25+CD127− T-cells, whereas CD127 levels on CD8+ T-cells were significantly correlated to concentrations of VEGF and triglycerides. Our data indicate that CD127 expression is differentially modulated on CD4+ and CD8+ T-cells in the course of T1D. Moreover, we demonstrated that, in contrast to recent-onset T1D, long-lasting T1D is associated with enhancement of T-cells with regulatory phenotype.http://dx.doi.org/10.1155/2013/459210 |
| spellingShingle | Marcin Moniuszko Barbara Glowinska-Olszewska Malgorzata Rusak Marta Jeznach Kamil Grubczak Danuta Lipinska Robert Milewski Anna Justyna Milewska Milena Dabrowska Ewa Jablonska Adam Kretowski Maria Gorska Anna Bodzenta-Lukaszyk Artur Bossowski Decreased CD127 Expression on CD4+ T-Cells and Elevated Frequencies of CD4+CD25+CD127− T-Cells in Children with Long-Lasting Type 1 Diabetes Clinical and Developmental Immunology |
| title | Decreased CD127 Expression on CD4+ T-Cells and Elevated Frequencies of CD4+CD25+CD127− T-Cells in Children with Long-Lasting Type 1 Diabetes |
| title_full | Decreased CD127 Expression on CD4+ T-Cells and Elevated Frequencies of CD4+CD25+CD127− T-Cells in Children with Long-Lasting Type 1 Diabetes |
| title_fullStr | Decreased CD127 Expression on CD4+ T-Cells and Elevated Frequencies of CD4+CD25+CD127− T-Cells in Children with Long-Lasting Type 1 Diabetes |
| title_full_unstemmed | Decreased CD127 Expression on CD4+ T-Cells and Elevated Frequencies of CD4+CD25+CD127− T-Cells in Children with Long-Lasting Type 1 Diabetes |
| title_short | Decreased CD127 Expression on CD4+ T-Cells and Elevated Frequencies of CD4+CD25+CD127− T-Cells in Children with Long-Lasting Type 1 Diabetes |
| title_sort | decreased cd127 expression on cd4 t cells and elevated frequencies of cd4 cd25 cd127 t cells in children with long lasting type 1 diabetes |
| url | http://dx.doi.org/10.1155/2013/459210 |
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