1.4 nm gold nanoparticle-antibody conjugates for in situ gold immunolabelling after transduction into living human cells
Despite advances in Electron Microscopy (EM) that enable to image protein assemblies within vitreous sections of cells at nearly atomic resolution, labelling is still necessary to locate small proteins or rare complexes. Gold immunolabelling has been used for decades to localise specific proteins wi...
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| Format: | Article |
| Language: | English |
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Académie des sciences
2023-10-01
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| Series: | Comptes Rendus. Chimie |
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| Online Access: | https://comptes-rendus.academie-sciences.fr/chimie/articles/10.5802/crchim.251/ |
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| author | Groysbeck, Nadja Haeberlé, Anne Marie Ory, Stéphane Hanss, Victor Eltsov, Mikhael Schultz, Patrick Zuber, Guy |
| author_facet | Groysbeck, Nadja Haeberlé, Anne Marie Ory, Stéphane Hanss, Victor Eltsov, Mikhael Schultz, Patrick Zuber, Guy |
| author_sort | Groysbeck, Nadja |
| collection | DOAJ |
| description | Despite advances in Electron Microscopy (EM) that enable to image protein assemblies within vitreous sections of cells at nearly atomic resolution, labelling is still necessary to locate small proteins or rare complexes. Gold immunolabelling has been used for decades to localise specific proteins within cellular sections. However, current gold particle-antibody conjugates are not built with enough chemical precision to match the current resolution offered by cryo-EM methodology. Furthermore, as a close to native specimen state can only be achieved by strict preservation of a frozen hydrated state, it is required to deliver gold labelling agents into living cells prior to their vitrification. Several 1.4 nm gold nanoparticle-antibody conjugates were synthesised. Their abilities to bind to and label their corresponding epitopes within living cells after cytosolic delivery by electroporation are documented here. |
| format | Article |
| id | doaj-art-f7409895d18f4bc28a8ff3d3826b03ce |
| institution | Kabale University |
| issn | 1878-1543 |
| language | English |
| publishDate | 2023-10-01 |
| publisher | Académie des sciences |
| record_format | Article |
| series | Comptes Rendus. Chimie |
| spelling | doaj-art-f7409895d18f4bc28a8ff3d3826b03ce2025-02-07T13:38:54ZengAcadémie des sciencesComptes Rendus. Chimie1878-15432023-10-0126S3536610.5802/crchim.25110.5802/crchim.2511.4 nm gold nanoparticle-antibody conjugates for in situ gold immunolabelling after transduction into living human cellsGroysbeck, Nadja0https://orcid.org/0000-0002-8672-555XHaeberlé, Anne Marie1Ory, Stéphane2https://orcid.org/0000-0003-4359-1157Hanss, Victor3https://orcid.org/0000-0001-5603-8321Eltsov, Mikhael4https://orcid.org/0000-0003-2298-8964Schultz, Patrick5https://orcid.org/0000-0002-7310-6186Zuber, Guy6https://orcid.org/0000-0001-8032-2410Université de Strasbourg - CNRS, UMR7242 Biotechnologie et Signalisation Cellulaire, 300 Bd Sébastien Brant, CS 10413, 67412 Illkirch, FranceCentre National de la Recherche Scientifique, Université de Strasbourg, Institut des Neurosciences Cellulaires et Intégratives, F-67000 Strasbourg, FranceCentre National de la Recherche Scientifique, Université de Strasbourg, Institut des Neurosciences Cellulaires et Intégratives, F-67000 Strasbourg, FranceCentre for Integrative Biology (CBI), Department of Integrated Structural Biology, Institut de Génétique et de Biologie Moléculaire et Cellulaire (IGBMC), 1 rue Laurent Fries, BP10142, F-67404 Illkirch Cedex, FranceCentre for Integrative Biology (CBI), Department of Integrated Structural Biology, Institut de Génétique et de Biologie Moléculaire et Cellulaire (IGBMC), 1 rue Laurent Fries, BP10142, F-67404 Illkirch Cedex, FranceCentre for Integrative Biology (CBI), Department of Integrated Structural Biology, Institut de Génétique et de Biologie Moléculaire et Cellulaire (IGBMC), 1 rue Laurent Fries, BP10142, F-67404 Illkirch Cedex, FranceUniversité de Strasbourg - CNRS, UMR7242 Biotechnologie et Signalisation Cellulaire, 300 Bd Sébastien Brant, CS 10413, 67412 Illkirch, FranceDespite advances in Electron Microscopy (EM) that enable to image protein assemblies within vitreous sections of cells at nearly atomic resolution, labelling is still necessary to locate small proteins or rare complexes. Gold immunolabelling has been used for decades to localise specific proteins within cellular sections. However, current gold particle-antibody conjugates are not built with enough chemical precision to match the current resolution offered by cryo-EM methodology. Furthermore, as a close to native specimen state can only be achieved by strict preservation of a frozen hydrated state, it is required to deliver gold labelling agents into living cells prior to their vitrification. Several 1.4 nm gold nanoparticle-antibody conjugates were synthesised. Their abilities to bind to and label their corresponding epitopes within living cells after cytosolic delivery by electroporation are documented here.https://comptes-rendus.academie-sciences.fr/chimie/articles/10.5802/crchim.251/Gold nanoparticleAntibodyConjugateIntracellular deliveryGold immunolabelling |
| spellingShingle | Groysbeck, Nadja Haeberlé, Anne Marie Ory, Stéphane Hanss, Victor Eltsov, Mikhael Schultz, Patrick Zuber, Guy 1.4 nm gold nanoparticle-antibody conjugates for in situ gold immunolabelling after transduction into living human cells Comptes Rendus. Chimie Gold nanoparticle Antibody Conjugate Intracellular delivery Gold immunolabelling |
| title | 1.4 nm gold nanoparticle-antibody conjugates for in situ gold immunolabelling after transduction into living human cells |
| title_full | 1.4 nm gold nanoparticle-antibody conjugates for in situ gold immunolabelling after transduction into living human cells |
| title_fullStr | 1.4 nm gold nanoparticle-antibody conjugates for in situ gold immunolabelling after transduction into living human cells |
| title_full_unstemmed | 1.4 nm gold nanoparticle-antibody conjugates for in situ gold immunolabelling after transduction into living human cells |
| title_short | 1.4 nm gold nanoparticle-antibody conjugates for in situ gold immunolabelling after transduction into living human cells |
| title_sort | 1 4 nm gold nanoparticle antibody conjugates for in situ gold immunolabelling after transduction into living human cells |
| topic | Gold nanoparticle Antibody Conjugate Intracellular delivery Gold immunolabelling |
| url | https://comptes-rendus.academie-sciences.fr/chimie/articles/10.5802/crchim.251/ |
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