Evaluation of TaMFT-3A and TaMKK3-4A alleles on wheat pre-harvest sprouting
Pre‐harvest sprouting (PHS) is a significant challenge affecting global production of wheat (Triticum aestivum L.). Resistance to PHS is governed by both genetic and environmental factors, making reliable molecular markers essential for enhancing PHS resistance through molecular marker‐assisted sele...
Saved in:
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2025-05-01
|
| Series: | Frontiers in Plant Science |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2025.1594385/full |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850136365227835392 |
|---|---|
| author | Bo-Wen Zhang Bo-Wen Zhang Bai-Song Yang Xiao-Neng Wan Xin Ma Kai-Di Lyu Han Wang Shu-Ying Yang Hui-Hui Zhang Shu-Nv Hao Jian Ma Guo-Zhong Sun |
| author_facet | Bo-Wen Zhang Bo-Wen Zhang Bai-Song Yang Xiao-Neng Wan Xin Ma Kai-Di Lyu Han Wang Shu-Ying Yang Hui-Hui Zhang Shu-Nv Hao Jian Ma Guo-Zhong Sun |
| author_sort | Bo-Wen Zhang |
| collection | DOAJ |
| description | Pre‐harvest sprouting (PHS) is a significant challenge affecting global production of wheat (Triticum aestivum L.). Resistance to PHS is governed by both genetic and environmental factors, making reliable molecular markers essential for enhancing PHS resistance through molecular marker‐assisted selection (MAS). Genes TaMFT-3A and TaMKK3-4A have been cloned in wheat and are known to regulate PHS resistance in white-grained varieties. In this study, we assessed the allelic variations in these genes and their combined effects on PHS resistance using two recombinant inbred line (RIL) populations, Wanxianbaimaizi/Zhongyou 9507 (WZ) and Wanxianbaimaizi/Jing 411 (WJ), under two distinct field environmental conditions. PHS resistance was assessed by measuring seed germination in physiologically mature stage, correlation and ANOVA were used to analyze PHS data. The germination percentage (GP) and germination index (GI) were significantly correlated across both RIL populations. Specific allelic variations at positions -222, +646, and +666 in the TaMFT-3A gene strongly correlated with PHS resistance. The CGA haplotype at these loci was linked to the highest resistance, while the TAA haplotype was associated with the lowest resistance levels. Additionally, haplotype variation at the +660 locus of TaMKK3-4A demonstrated a weak but environmentally modulated correlation with PHS resistance. This study provides a theoretical foundation for utilizing TaMFT-3A and TaMKK3-4A in molecular breeding strategies to enhance wheat resilience to PHS. |
| format | Article |
| id | doaj-art-f7301d8edf124de683335331c745d6ff |
| institution | OA Journals |
| issn | 1664-462X |
| language | English |
| publishDate | 2025-05-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj-art-f7301d8edf124de683335331c745d6ff2025-08-20T02:31:09ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2025-05-011610.3389/fpls.2025.15943851594385Evaluation of TaMFT-3A and TaMKK3-4A alleles on wheat pre-harvest sproutingBo-Wen Zhang0Bo-Wen Zhang1Bai-Song Yang2Xiao-Neng Wan3Xin Ma4Kai-Di Lyu5Han Wang6Shu-Ying Yang7Hui-Hui Zhang8Shu-Nv Hao9Jian Ma10Guo-Zhong Sun11College of Agronomy, Jilin Agricultural University, Changchun, ChinaNational Engineering Research Center for Crop Molecular Breeding, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences (CAAS), Beijing, ChinaNational Engineering Research Center for Crop Molecular Breeding, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences (CAAS), Beijing, ChinaNational Engineering Research Center for Crop Molecular Breeding, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences (CAAS), Beijing, ChinaNational Engineering Research Center for Crop Molecular Breeding, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences (CAAS), Beijing, ChinaNational Engineering Research Center for Crop Molecular Breeding, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences (CAAS), Beijing, ChinaNational Engineering Research Center for Crop Molecular Breeding, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences (CAAS), Beijing, ChinaNational Engineering Research Center for Crop Molecular Breeding, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences (CAAS), Beijing, ChinaNational Engineering Research Center for Crop Molecular Breeding, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences (CAAS), Beijing, ChinaNational Engineering Research Center for Crop Molecular Breeding, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences (CAAS), Beijing, ChinaCollege of Agronomy, Jilin Agricultural University, Changchun, ChinaNational Engineering Research Center for Crop Molecular Breeding, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences (CAAS), Beijing, ChinaPre‐harvest sprouting (PHS) is a significant challenge affecting global production of wheat (Triticum aestivum L.). Resistance to PHS is governed by both genetic and environmental factors, making reliable molecular markers essential for enhancing PHS resistance through molecular marker‐assisted selection (MAS). Genes TaMFT-3A and TaMKK3-4A have been cloned in wheat and are known to regulate PHS resistance in white-grained varieties. In this study, we assessed the allelic variations in these genes and their combined effects on PHS resistance using two recombinant inbred line (RIL) populations, Wanxianbaimaizi/Zhongyou 9507 (WZ) and Wanxianbaimaizi/Jing 411 (WJ), under two distinct field environmental conditions. PHS resistance was assessed by measuring seed germination in physiologically mature stage, correlation and ANOVA were used to analyze PHS data. The germination percentage (GP) and germination index (GI) were significantly correlated across both RIL populations. Specific allelic variations at positions -222, +646, and +666 in the TaMFT-3A gene strongly correlated with PHS resistance. The CGA haplotype at these loci was linked to the highest resistance, while the TAA haplotype was associated with the lowest resistance levels. Additionally, haplotype variation at the +660 locus of TaMKK3-4A demonstrated a weak but environmentally modulated correlation with PHS resistance. This study provides a theoretical foundation for utilizing TaMFT-3A and TaMKK3-4A in molecular breeding strategies to enhance wheat resilience to PHS.https://www.frontiersin.org/articles/10.3389/fpls.2025.1594385/fullwheatpre-harvest spoutingrecombinant inbred linehaplotypemarker-assisted selection |
| spellingShingle | Bo-Wen Zhang Bo-Wen Zhang Bai-Song Yang Xiao-Neng Wan Xin Ma Kai-Di Lyu Han Wang Shu-Ying Yang Hui-Hui Zhang Shu-Nv Hao Jian Ma Guo-Zhong Sun Evaluation of TaMFT-3A and TaMKK3-4A alleles on wheat pre-harvest sprouting Frontiers in Plant Science wheat pre-harvest spouting recombinant inbred line haplotype marker-assisted selection |
| title | Evaluation of TaMFT-3A and TaMKK3-4A alleles on wheat pre-harvest sprouting |
| title_full | Evaluation of TaMFT-3A and TaMKK3-4A alleles on wheat pre-harvest sprouting |
| title_fullStr | Evaluation of TaMFT-3A and TaMKK3-4A alleles on wheat pre-harvest sprouting |
| title_full_unstemmed | Evaluation of TaMFT-3A and TaMKK3-4A alleles on wheat pre-harvest sprouting |
| title_short | Evaluation of TaMFT-3A and TaMKK3-4A alleles on wheat pre-harvest sprouting |
| title_sort | evaluation of tamft 3a and tamkk3 4a alleles on wheat pre harvest sprouting |
| topic | wheat pre-harvest spouting recombinant inbred line haplotype marker-assisted selection |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2025.1594385/full |
| work_keys_str_mv | AT bowenzhang evaluationoftamft3aandtamkk34aallelesonwheatpreharvestsprouting AT bowenzhang evaluationoftamft3aandtamkk34aallelesonwheatpreharvestsprouting AT baisongyang evaluationoftamft3aandtamkk34aallelesonwheatpreharvestsprouting AT xiaonengwan evaluationoftamft3aandtamkk34aallelesonwheatpreharvestsprouting AT xinma evaluationoftamft3aandtamkk34aallelesonwheatpreharvestsprouting AT kaidilyu evaluationoftamft3aandtamkk34aallelesonwheatpreharvestsprouting AT hanwang evaluationoftamft3aandtamkk34aallelesonwheatpreharvestsprouting AT shuyingyang evaluationoftamft3aandtamkk34aallelesonwheatpreharvestsprouting AT huihuizhang evaluationoftamft3aandtamkk34aallelesonwheatpreharvestsprouting AT shunvhao evaluationoftamft3aandtamkk34aallelesonwheatpreharvestsprouting AT jianma evaluationoftamft3aandtamkk34aallelesonwheatpreharvestsprouting AT guozhongsun evaluationoftamft3aandtamkk34aallelesonwheatpreharvestsprouting |