Molecular characterization of Pseudomyxoma peritonei with single-cell and bulk RNA sequencing
Abstract Pseudomyxoma peritonei (PMP), a rare condition characterized by mucinous ascites in the peritoneal cavity, often leads to a poor prognosis. However, omics profiling of this disease remains significantly underexplored. Here, we present single-cell transcriptomic profiling of five PMP cases t...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2025-02-01
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| Series: | Scientific Data |
| Online Access: | https://doi.org/10.1038/s41597-025-04561-4 |
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| author | Ye Jin Ha Seong-Hwan Park Seon-Kyu Kim Ka Hee Tak Jeong-Hwan Kim Chan Wook Kim Yong Sik Yoon Seon-Young Kim Jong Lyul Lee |
| author_facet | Ye Jin Ha Seong-Hwan Park Seon-Kyu Kim Ka Hee Tak Jeong-Hwan Kim Chan Wook Kim Yong Sik Yoon Seon-Young Kim Jong Lyul Lee |
| author_sort | Ye Jin Ha |
| collection | DOAJ |
| description | Abstract Pseudomyxoma peritonei (PMP), a rare condition characterized by mucinous ascites in the peritoneal cavity, often leads to a poor prognosis. However, omics profiling of this disease remains significantly underexplored. Here, we present single-cell transcriptomic profiling of five PMP cases to identify cell type-specific gene features associated with PMP pathogenesis. Additionally, we provide bulk RNA-seq datasets from two independent cohorts: 19 fresh frozen tissue samples (12 PMPs) and 34 formalin-fixed paraffin-embedded (FFPE) samples (25 PMPs). We also offer protein expression data from a tissue microarray (TMA) analysis of 90 samples (45 PMPs). Our single-cell and bulk transcriptomic profiles, along with TMA verifications, reveal the cellular diversity of PMP, highlighting the coexistence of epithelial and mesenchymal characteristics within PMP cells. These datasets enhance our understanding of PMP pathogenesis and provide a valuable resource for uncovering the intricate molecular landscape of PMP, with the potential to improve clinical utility through further research. |
| format | Article |
| id | doaj-art-f70ec639624a4a84be1f751cbdfd515c |
| institution | Kabale University |
| issn | 2052-4463 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Data |
| spelling | doaj-art-f70ec639624a4a84be1f751cbdfd515c2025-02-09T12:11:49ZengNature PortfolioScientific Data2052-44632025-02-011211810.1038/s41597-025-04561-4Molecular characterization of Pseudomyxoma peritonei with single-cell and bulk RNA sequencingYe Jin Ha0Seong-Hwan Park1Seon-Kyu Kim2Ka Hee Tak3Jeong-Hwan Kim4Chan Wook Kim5Yong Sik Yoon6Seon-Young Kim7Jong Lyul Lee8Asan Institute for Life Sciences, Asan Medical CenterAging Convergence Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB)Aging Convergence Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB)Asan Institute for Life Sciences, Asan Medical CenterAging Convergence Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB)Asan Institute for Life Sciences, Asan Medical CenterAsan Institute for Life Sciences, Asan Medical CenterDepartment of Bioscience, University of Science and TechnologyAsan Institute for Life Sciences, Asan Medical CenterAbstract Pseudomyxoma peritonei (PMP), a rare condition characterized by mucinous ascites in the peritoneal cavity, often leads to a poor prognosis. However, omics profiling of this disease remains significantly underexplored. Here, we present single-cell transcriptomic profiling of five PMP cases to identify cell type-specific gene features associated with PMP pathogenesis. Additionally, we provide bulk RNA-seq datasets from two independent cohorts: 19 fresh frozen tissue samples (12 PMPs) and 34 formalin-fixed paraffin-embedded (FFPE) samples (25 PMPs). We also offer protein expression data from a tissue microarray (TMA) analysis of 90 samples (45 PMPs). Our single-cell and bulk transcriptomic profiles, along with TMA verifications, reveal the cellular diversity of PMP, highlighting the coexistence of epithelial and mesenchymal characteristics within PMP cells. These datasets enhance our understanding of PMP pathogenesis and provide a valuable resource for uncovering the intricate molecular landscape of PMP, with the potential to improve clinical utility through further research.https://doi.org/10.1038/s41597-025-04561-4 |
| spellingShingle | Ye Jin Ha Seong-Hwan Park Seon-Kyu Kim Ka Hee Tak Jeong-Hwan Kim Chan Wook Kim Yong Sik Yoon Seon-Young Kim Jong Lyul Lee Molecular characterization of Pseudomyxoma peritonei with single-cell and bulk RNA sequencing Scientific Data |
| title | Molecular characterization of Pseudomyxoma peritonei with single-cell and bulk RNA sequencing |
| title_full | Molecular characterization of Pseudomyxoma peritonei with single-cell and bulk RNA sequencing |
| title_fullStr | Molecular characterization of Pseudomyxoma peritonei with single-cell and bulk RNA sequencing |
| title_full_unstemmed | Molecular characterization of Pseudomyxoma peritonei with single-cell and bulk RNA sequencing |
| title_short | Molecular characterization of Pseudomyxoma peritonei with single-cell and bulk RNA sequencing |
| title_sort | molecular characterization of pseudomyxoma peritonei with single cell and bulk rna sequencing |
| url | https://doi.org/10.1038/s41597-025-04561-4 |
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