Evaluation of Aromatic Boronic Acids as Ligands for Measuring Diabetes Markers on Carbon Nanotube Field-Effect Transistors
Biomolecular detections performed on carbon nanotube field-effect transistors (CNT-FETs) frequently use reactive pyrenes as an anchor to tether bioactive ligands to the hydrophobic nanotubes. In this paper, we explore the possibility of directly using bioactive aromatic compounds themselves as CNT-F...
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| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2012-01-01
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| Series: | Journal of Nanotechnology |
| Online Access: | http://dx.doi.org/10.1155/2012/371487 |
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| author | Steingrimur Stefansson Lára A. Stefansson Suk-won Chung Kevin Ko Hena H. Kwon Saeyoung Nate Ahn |
| author_facet | Steingrimur Stefansson Lára A. Stefansson Suk-won Chung Kevin Ko Hena H. Kwon Saeyoung Nate Ahn |
| author_sort | Steingrimur Stefansson |
| collection | DOAJ |
| description | Biomolecular detections performed on carbon nanotube field-effect transistors (CNT-FETs) frequently use reactive pyrenes as an anchor to tether bioactive ligands to the hydrophobic nanotubes. In this paper, we explore the possibility of directly using bioactive aromatic compounds themselves as CNT-FET ligands. This would be an efficient way to functionalize CNT-FETs since many aromatic compounds bind avidly to nanotubes, and it would also ensure that ligand-binding molecules would be brought in close proximity to the nanotubes. Using a model system consisting of pyrene, phenanthrene, naphthalene, or phenyl boronic acids immobilized on CNT-FET wafers, we show that all are able to bind glycated human serum albumin (gHSA), which is an important diabetes marker. Pyrene boronic acid proved to bind CNTs with the greatest apparent affinity as measured by gHSA impedance. Interestingly, gHSA CNT-FET signal intensity, which is proportional to amount of protein bound, remained essentially unchanged for all the boronic acids tested. |
| format | Article |
| id | doaj-art-f70ca9bc79e5492db0df3c629634d059 |
| institution | Kabale University |
| issn | 1687-9503 1687-9511 |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Nanotechnology |
| spelling | doaj-art-f70ca9bc79e5492db0df3c629634d0592025-02-03T01:23:01ZengWileyJournal of Nanotechnology1687-95031687-95112012-01-01201210.1155/2012/371487371487Evaluation of Aromatic Boronic Acids as Ligands for Measuring Diabetes Markers on Carbon Nanotube Field-Effect TransistorsSteingrimur Stefansson0Lára A. Stefansson1Suk-won Chung2Kevin Ko3Hena H. Kwon4Saeyoung Nate Ahn5Fuzbien Technology Institute, 9700 Great Seneca Highway, Suite 302, Rockville, MD 20850, USAMagruder High School, 5939 Muncaster Mill Road, Rockville, MD 20855, USACentreville High School, 6001 Union Mill Road, Clifton, VA 20124, USAThomas Jefferson High School for Science and Technology, 6560 Braddock Road, Alexandria, VA 22312, USAMediSourcePlus Co., Ltd., 222-22 Gurodong, Gurogu, Seoul 336-708, Republic of KoreaFuzbien Technology Institute, 9700 Great Seneca Highway, Suite 302, Rockville, MD 20850, USABiomolecular detections performed on carbon nanotube field-effect transistors (CNT-FETs) frequently use reactive pyrenes as an anchor to tether bioactive ligands to the hydrophobic nanotubes. In this paper, we explore the possibility of directly using bioactive aromatic compounds themselves as CNT-FET ligands. This would be an efficient way to functionalize CNT-FETs since many aromatic compounds bind avidly to nanotubes, and it would also ensure that ligand-binding molecules would be brought in close proximity to the nanotubes. Using a model system consisting of pyrene, phenanthrene, naphthalene, or phenyl boronic acids immobilized on CNT-FET wafers, we show that all are able to bind glycated human serum albumin (gHSA), which is an important diabetes marker. Pyrene boronic acid proved to bind CNTs with the greatest apparent affinity as measured by gHSA impedance. Interestingly, gHSA CNT-FET signal intensity, which is proportional to amount of protein bound, remained essentially unchanged for all the boronic acids tested.http://dx.doi.org/10.1155/2012/371487 |
| spellingShingle | Steingrimur Stefansson Lára A. Stefansson Suk-won Chung Kevin Ko Hena H. Kwon Saeyoung Nate Ahn Evaluation of Aromatic Boronic Acids as Ligands for Measuring Diabetes Markers on Carbon Nanotube Field-Effect Transistors Journal of Nanotechnology |
| title | Evaluation of Aromatic Boronic Acids as Ligands for Measuring Diabetes Markers on Carbon Nanotube Field-Effect Transistors |
| title_full | Evaluation of Aromatic Boronic Acids as Ligands for Measuring Diabetes Markers on Carbon Nanotube Field-Effect Transistors |
| title_fullStr | Evaluation of Aromatic Boronic Acids as Ligands for Measuring Diabetes Markers on Carbon Nanotube Field-Effect Transistors |
| title_full_unstemmed | Evaluation of Aromatic Boronic Acids as Ligands for Measuring Diabetes Markers on Carbon Nanotube Field-Effect Transistors |
| title_short | Evaluation of Aromatic Boronic Acids as Ligands for Measuring Diabetes Markers on Carbon Nanotube Field-Effect Transistors |
| title_sort | evaluation of aromatic boronic acids as ligands for measuring diabetes markers on carbon nanotube field effect transistors |
| url | http://dx.doi.org/10.1155/2012/371487 |
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