Melittin Inhibition and Eradication Activity for Resistant Polymicrobial Biofilm Isolated from a Dairy Industry after Disinfection
The emerging concern about the increase of antibiotic resistance and associated biofilm has encouraged scientists to look for alternative antibiotics such as antimicrobial peptides (AMPs). This study evaluated the ability of melittin to act as an antibacterial biofilm inhibitor and biofilm remover c...
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| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2019-01-01
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| Series: | International Journal of Microbiology |
| Online Access: | http://dx.doi.org/10.1155/2019/4012394 |
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| _version_ | 1850161499800076288 |
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| author | Emilia Galdiero Antonietta Siciliano Renato Gesuele Valeria Di Onofrio Annarita Falanga Angela Maione Renato Liguori Giovanni Libralato Marco Guida |
| author_facet | Emilia Galdiero Antonietta Siciliano Renato Gesuele Valeria Di Onofrio Annarita Falanga Angela Maione Renato Liguori Giovanni Libralato Marco Guida |
| author_sort | Emilia Galdiero |
| collection | DOAJ |
| description | The emerging concern about the increase of antibiotic resistance and associated biofilm has encouraged scientists to look for alternative antibiotics such as antimicrobial peptides (AMPs). This study evaluated the ability of melittin to act as an antibacterial biofilm inhibitor and biofilm remover considering isolates from dairy industry. Minimum inhibitory concentrations (MICs), minimum bactericidal concentrations (MBCs), minimum biofilm inhibitory concentrations (MBICs), and biofilm removal activities were studied in polymicrobial biofilms produced from isolates. MIC and MBC were set at 1–3 µg/mL and 25–50 µg/mL for Gram-positive and Gram-negative bacteria, respectively. Results demonstrated a good MBIC reaching 85% inhibition ability and a good activity and better penetration in deeper layers against the mixed preformed biofilm, thereby increasing its activity against all isolates also at the lowest tested concentrations. Melittin showed interesting characteristics suggesting its potential to act as an antimicrobial agent for polymicrobial biofilm from dairy industry even in environmental isolates. |
| format | Article |
| id | doaj-art-f700d6c05a6e45f184f152e8d6488ea3 |
| institution | OA Journals |
| issn | 1687-918X 1687-9198 |
| language | English |
| publishDate | 2019-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Microbiology |
| spelling | doaj-art-f700d6c05a6e45f184f152e8d6488ea32025-08-20T02:22:49ZengWileyInternational Journal of Microbiology1687-918X1687-91982019-01-01201910.1155/2019/40123944012394Melittin Inhibition and Eradication Activity for Resistant Polymicrobial Biofilm Isolated from a Dairy Industry after DisinfectionEmilia Galdiero0Antonietta Siciliano1Renato Gesuele2Valeria Di Onofrio3Annarita Falanga4Angela Maione5Renato Liguori6Giovanni Libralato7Marco Guida8Department of Biology, University of Naples Federico II, Via Cinthia, 80126 Naples, ItalyDepartment of Biology, University of Naples Federico II, Via Cinthia, 80126 Naples, ItalyDepartment of Biology, University of Naples Federico II, Via Cinthia, 80126 Naples, ItalyDepartment of Sciences and Technologies, University of Naples “Parthenope”, Business District, Block C4, Naples 80143, ItalyDepartment of Agricultural Science, University of Naples Federico II, Via Università 100, 80055 Portici, Napoli, ItalyDepartment of Biology, University of Naples Federico II, Via Cinthia, 80126 Naples, ItalyDepartment of Movement and Wellbeing Sciences, University of Naples “Parthenope”, Via Medina 40, Naples 80133, ItalyDepartment of Biology, University of Naples Federico II, Via Cinthia, 80126 Naples, ItalyDepartment of Biology, University of Naples Federico II, Via Cinthia, 80126 Naples, ItalyThe emerging concern about the increase of antibiotic resistance and associated biofilm has encouraged scientists to look for alternative antibiotics such as antimicrobial peptides (AMPs). This study evaluated the ability of melittin to act as an antibacterial biofilm inhibitor and biofilm remover considering isolates from dairy industry. Minimum inhibitory concentrations (MICs), minimum bactericidal concentrations (MBCs), minimum biofilm inhibitory concentrations (MBICs), and biofilm removal activities were studied in polymicrobial biofilms produced from isolates. MIC and MBC were set at 1–3 µg/mL and 25–50 µg/mL for Gram-positive and Gram-negative bacteria, respectively. Results demonstrated a good MBIC reaching 85% inhibition ability and a good activity and better penetration in deeper layers against the mixed preformed biofilm, thereby increasing its activity against all isolates also at the lowest tested concentrations. Melittin showed interesting characteristics suggesting its potential to act as an antimicrobial agent for polymicrobial biofilm from dairy industry even in environmental isolates.http://dx.doi.org/10.1155/2019/4012394 |
| spellingShingle | Emilia Galdiero Antonietta Siciliano Renato Gesuele Valeria Di Onofrio Annarita Falanga Angela Maione Renato Liguori Giovanni Libralato Marco Guida Melittin Inhibition and Eradication Activity for Resistant Polymicrobial Biofilm Isolated from a Dairy Industry after Disinfection International Journal of Microbiology |
| title | Melittin Inhibition and Eradication Activity for Resistant Polymicrobial Biofilm Isolated from a Dairy Industry after Disinfection |
| title_full | Melittin Inhibition and Eradication Activity for Resistant Polymicrobial Biofilm Isolated from a Dairy Industry after Disinfection |
| title_fullStr | Melittin Inhibition and Eradication Activity for Resistant Polymicrobial Biofilm Isolated from a Dairy Industry after Disinfection |
| title_full_unstemmed | Melittin Inhibition and Eradication Activity for Resistant Polymicrobial Biofilm Isolated from a Dairy Industry after Disinfection |
| title_short | Melittin Inhibition and Eradication Activity for Resistant Polymicrobial Biofilm Isolated from a Dairy Industry after Disinfection |
| title_sort | melittin inhibition and eradication activity for resistant polymicrobial biofilm isolated from a dairy industry after disinfection |
| url | http://dx.doi.org/10.1155/2019/4012394 |
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