Development of targeted nanocapsules using pomegranate peel extract with enhanced bioavailability and anti-inflammatory activity for ulcerative colitis: In vitro studies
The study aimed to establish an accurate drug delivery system with a tailored mechanism for the efficient management of Ulcerative Colitis (UC). Using the solvent evaporation technique, nanoencapsulation of pomegranate peel extract (PPE) in biodegradable enteric polymer methacrylic acid-ethyl acryla...
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Elsevier
2024-12-01
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| Series: | Hybrid Advances |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2773207X24001957 |
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| author | P. Monika M.N. Chandraprabha R. Hari Krishna Vittal Maanya C. Likhitha N. Pooja |
| author_facet | P. Monika M.N. Chandraprabha R. Hari Krishna Vittal Maanya C. Likhitha N. Pooja |
| author_sort | P. Monika |
| collection | DOAJ |
| description | The study aimed to establish an accurate drug delivery system with a tailored mechanism for the efficient management of Ulcerative Colitis (UC). Using the solvent evaporation technique, nanoencapsulation of pomegranate peel extract (PPE) in biodegradable enteric polymer methacrylic acid-ethyl acrylate copolymer (Eudragit L-100) was prepared. In vitro drug release investigations indicated a greater percentage of drug released at 6.8 pH in contrast to 7.4 pH (96.98 ± 0.80 % vs. 93.97 ± 0.711 %), indicating its applicability in the region of the human intestine. The mechanism of drug release from the nanoformulations (NFR) exhibited a process controlled by Fickian diffusion. The results suggest efficient encapsulation of PPE within Eudragit L100 and may be used for targeted drug release in the intestinal region. Anti-inflammatory studies on the HIEC-6 cell line showed that tumour necrosis factor-α (TNF-α) expression was significantly (p < 0.001) decreased in all NFRs. Additionally, NFRs exhibited antioxidant activity and enhanced in vitro bioavailability (in intestinal pH compared to gastric pH). |
| format | Article |
| id | doaj-art-f6bcd172f8af48f1a135a6c962eedc72 |
| institution | DOAJ |
| issn | 2773-207X |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Hybrid Advances |
| spelling | doaj-art-f6bcd172f8af48f1a135a6c962eedc722025-08-20T02:50:08ZengElsevierHybrid Advances2773-207X2024-12-01710033410.1016/j.hybadv.2024.100334Development of targeted nanocapsules using pomegranate peel extract with enhanced bioavailability and anti-inflammatory activity for ulcerative colitis: In vitro studiesP. Monika0M.N. Chandraprabha1R. Hari Krishna2Vittal Maanya3C. Likhitha4N. Pooja5Department of Biotechnology, M.S. Ramaiah Institute of Technology (Affiliated to Visvesvaraya Technological University), MSR Nagar, MSRIT Post, Bangalore, 560054, India; Corresponding author.Department of Biotechnology, M.S. Ramaiah Institute of Technology (Affiliated to Visvesvaraya Technological University), MSR Nagar, MSRIT Post, Bangalore, 560054, India; Centre for Bio and Energy Materials Innovation, M.S. Ramaiah Institute of Technology (Affiliated to Visvesvaraya Technological University), MSR Nagar, MSRIT Post, Bangalore, 560054, India; Corresponding author. Department of Biotechnology, M.S. Ramaiah Institute of Technology (Affiliated to Visvesvaraya Technological University), MSR Nagar, MSRIT Post, Bangalore, 560054, India.Centre for Bio and Energy Materials Innovation, M.S. Ramaiah Institute of Technology (Affiliated to Visvesvaraya Technological University), MSR Nagar, MSRIT Post, Bangalore, 560054, India; Department of Chemistry, M.S. Ramaiah Institute of Technology (Affiliated to Visvesvaraya Technological University), MSR Nagar, MSRIT Post, Bangalore, 560054, India; Corresponding author. Centre for Bio and Energy Materials Innovation, M.S. Ramaiah Institute of Technology (Affiliated to Visvesvaraya Technological University), MSR Nagar, MSRIT Post, Bangalore, 560054, India.Department of Biotechnology, M.S. Ramaiah Institute of Technology (Affiliated to Visvesvaraya Technological University), MSR Nagar, MSRIT Post, Bangalore, 560054, IndiaDepartment of Biotechnology, M.S. Ramaiah Institute of Technology (Affiliated to Visvesvaraya Technological University), MSR Nagar, MSRIT Post, Bangalore, 560054, IndiaDepartment of Biotechnology, M.S. Ramaiah Institute of Technology (Affiliated to Visvesvaraya Technological University), MSR Nagar, MSRIT Post, Bangalore, 560054, IndiaThe study aimed to establish an accurate drug delivery system with a tailored mechanism for the efficient management of Ulcerative Colitis (UC). Using the solvent evaporation technique, nanoencapsulation of pomegranate peel extract (PPE) in biodegradable enteric polymer methacrylic acid-ethyl acrylate copolymer (Eudragit L-100) was prepared. In vitro drug release investigations indicated a greater percentage of drug released at 6.8 pH in contrast to 7.4 pH (96.98 ± 0.80 % vs. 93.97 ± 0.711 %), indicating its applicability in the region of the human intestine. The mechanism of drug release from the nanoformulations (NFR) exhibited a process controlled by Fickian diffusion. The results suggest efficient encapsulation of PPE within Eudragit L100 and may be used for targeted drug release in the intestinal region. Anti-inflammatory studies on the HIEC-6 cell line showed that tumour necrosis factor-α (TNF-α) expression was significantly (p < 0.001) decreased in all NFRs. Additionally, NFRs exhibited antioxidant activity and enhanced in vitro bioavailability (in intestinal pH compared to gastric pH).http://www.sciencedirect.com/science/article/pii/S2773207X24001957Pomegranate peel extractEudragit L-100Ulcerative colitisBioavailabilityTargeted drug deliveryAnti-inflammatory activity |
| spellingShingle | P. Monika M.N. Chandraprabha R. Hari Krishna Vittal Maanya C. Likhitha N. Pooja Development of targeted nanocapsules using pomegranate peel extract with enhanced bioavailability and anti-inflammatory activity for ulcerative colitis: In vitro studies Hybrid Advances Pomegranate peel extract Eudragit L-100 Ulcerative colitis Bioavailability Targeted drug delivery Anti-inflammatory activity |
| title | Development of targeted nanocapsules using pomegranate peel extract with enhanced bioavailability and anti-inflammatory activity for ulcerative colitis: In vitro studies |
| title_full | Development of targeted nanocapsules using pomegranate peel extract with enhanced bioavailability and anti-inflammatory activity for ulcerative colitis: In vitro studies |
| title_fullStr | Development of targeted nanocapsules using pomegranate peel extract with enhanced bioavailability and anti-inflammatory activity for ulcerative colitis: In vitro studies |
| title_full_unstemmed | Development of targeted nanocapsules using pomegranate peel extract with enhanced bioavailability and anti-inflammatory activity for ulcerative colitis: In vitro studies |
| title_short | Development of targeted nanocapsules using pomegranate peel extract with enhanced bioavailability and anti-inflammatory activity for ulcerative colitis: In vitro studies |
| title_sort | development of targeted nanocapsules using pomegranate peel extract with enhanced bioavailability and anti inflammatory activity for ulcerative colitis in vitro studies |
| topic | Pomegranate peel extract Eudragit L-100 Ulcerative colitis Bioavailability Targeted drug delivery Anti-inflammatory activity |
| url | http://www.sciencedirect.com/science/article/pii/S2773207X24001957 |
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