Combining whole genome and transcriptome sequencing to analyze the pathogenic mechanism of Diplodia sapinea blight in Pinus sylvestris var. mongolica Litv.
Diplodia sapinea (= Sphaeropsis sapinea) is an opportunistic pathogen that usually lives in symbiosis (the coexistence of dissimilar organisms) with its host and can cause disease under extreme climatic or physiological stress. In this study, we generated a high-quality genome map of D. sapinea usin...
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Taylor & Francis Group
2025-12-01
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| Series: | Virulence |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/21505594.2025.2490216 |
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| author | Ruiqi Wang Yuting Wang Sina Fu Shixian Liao Tingbo Jiang Boru Zhou |
| author_facet | Ruiqi Wang Yuting Wang Sina Fu Shixian Liao Tingbo Jiang Boru Zhou |
| author_sort | Ruiqi Wang |
| collection | DOAJ |
| description | Diplodia sapinea (= Sphaeropsis sapinea) is an opportunistic pathogen that usually lives in symbiosis (the coexistence of dissimilar organisms) with its host and can cause disease under extreme climatic or physiological stress. In this study, we generated a high-quality genome map of D. sapinea using PacBio Circular Consensus Sequencing (CCS) technology and analysed the key disease-causing genes of D. sapinea by RNA sequencing (RNA-seq). In the study, a number of cell wall degrading enzyme genes were identified to be up-regulated during pathogen infection, which may be involved in biotic stress response in P. sylvestris var. mongolica Litv. It was also found that the expression of antioxidant-related genes, such as those involved in carotenoid biosynthesis, ascorbate and glutathione metabolism, was up-regulated in the P. s. var. mongolica Litv. after fungus infection. Differently expressed genes (DEGs) -based protein-protein interaction (PPI) network was constructed that included 163 pairs of significantly positively correlated proteins, forming three highly interacting gene clusters, and the PPI network was predicted to be associated with the replication and propagation processes of the fungus. These results provide important information for understanding the pathogenic mechanisms of Diplodia tip blight and developing control strategies in P. s. var. mongolica Litv. |
| format | Article |
| id | doaj-art-f6b00da3db37400a8e3d06ec0cf523e2 |
| institution | DOAJ |
| issn | 2150-5594 2150-5608 |
| language | English |
| publishDate | 2025-12-01 |
| publisher | Taylor & Francis Group |
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| series | Virulence |
| spelling | doaj-art-f6b00da3db37400a8e3d06ec0cf523e22025-08-20T03:10:17ZengTaylor & Francis GroupVirulence2150-55942150-56082025-12-0116110.1080/21505594.2025.2490216Combining whole genome and transcriptome sequencing to analyze the pathogenic mechanism of Diplodia sapinea blight in Pinus sylvestris var. mongolica Litv.Ruiqi Wang0Yuting Wang1Sina Fu2Shixian Liao3Tingbo Jiang4Boru Zhou5State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin, ChinaState Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin, ChinaState Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin, ChinaState Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin, ChinaState Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin, ChinaState Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin, ChinaDiplodia sapinea (= Sphaeropsis sapinea) is an opportunistic pathogen that usually lives in symbiosis (the coexistence of dissimilar organisms) with its host and can cause disease under extreme climatic or physiological stress. In this study, we generated a high-quality genome map of D. sapinea using PacBio Circular Consensus Sequencing (CCS) technology and analysed the key disease-causing genes of D. sapinea by RNA sequencing (RNA-seq). In the study, a number of cell wall degrading enzyme genes were identified to be up-regulated during pathogen infection, which may be involved in biotic stress response in P. sylvestris var. mongolica Litv. It was also found that the expression of antioxidant-related genes, such as those involved in carotenoid biosynthesis, ascorbate and glutathione metabolism, was up-regulated in the P. s. var. mongolica Litv. after fungus infection. Differently expressed genes (DEGs) -based protein-protein interaction (PPI) network was constructed that included 163 pairs of significantly positively correlated proteins, forming three highly interacting gene clusters, and the PPI network was predicted to be associated with the replication and propagation processes of the fungus. These results provide important information for understanding the pathogenic mechanisms of Diplodia tip blight and developing control strategies in P. s. var. mongolica Litv.https://www.tandfonline.com/doi/10.1080/21505594.2025.2490216Diplodia sapineafungipathogenicreactive oxygen speciesgenome sequencing |
| spellingShingle | Ruiqi Wang Yuting Wang Sina Fu Shixian Liao Tingbo Jiang Boru Zhou Combining whole genome and transcriptome sequencing to analyze the pathogenic mechanism of Diplodia sapinea blight in Pinus sylvestris var. mongolica Litv. Virulence Diplodia sapinea fungi pathogenic reactive oxygen species genome sequencing |
| title | Combining whole genome and transcriptome sequencing to analyze the pathogenic mechanism of Diplodia sapinea blight in Pinus sylvestris var. mongolica Litv. |
| title_full | Combining whole genome and transcriptome sequencing to analyze the pathogenic mechanism of Diplodia sapinea blight in Pinus sylvestris var. mongolica Litv. |
| title_fullStr | Combining whole genome and transcriptome sequencing to analyze the pathogenic mechanism of Diplodia sapinea blight in Pinus sylvestris var. mongolica Litv. |
| title_full_unstemmed | Combining whole genome and transcriptome sequencing to analyze the pathogenic mechanism of Diplodia sapinea blight in Pinus sylvestris var. mongolica Litv. |
| title_short | Combining whole genome and transcriptome sequencing to analyze the pathogenic mechanism of Diplodia sapinea blight in Pinus sylvestris var. mongolica Litv. |
| title_sort | combining whole genome and transcriptome sequencing to analyze the pathogenic mechanism of diplodia sapinea blight in pinus sylvestris var mongolica litv |
| topic | Diplodia sapinea fungi pathogenic reactive oxygen species genome sequencing |
| url | https://www.tandfonline.com/doi/10.1080/21505594.2025.2490216 |
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