Prevalence of Avian Pathogenic Escherichia coli (APEC) Clone Harboring sfa Gene in Brazil
Escherichia coli sfa+ strains isolated from poultry were serotyped and characterized by polymerase chain reaction (PCR) and amplified fragment length polymorphism (AFLP). Isolates collected from 12 Brazilian poultry farms mostly belonged to serogroup O6, followed by serogroups O2, O8, O21, O46, O78,...
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| Format: | Article |
| Language: | English |
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Wiley
2012-01-01
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| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1100/2012/437342 |
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| author | Terezinha Knöbl Andrea Micke Moreno Renata Paixão Tânia Aparecida Tardelli Gomes Mônica Aparecida Midolli Vieira Domingos da Silva Leite Jesus E. Blanco Antônio José Piantino Ferreira |
| author_facet | Terezinha Knöbl Andrea Micke Moreno Renata Paixão Tânia Aparecida Tardelli Gomes Mônica Aparecida Midolli Vieira Domingos da Silva Leite Jesus E. Blanco Antônio José Piantino Ferreira |
| author_sort | Terezinha Knöbl |
| collection | DOAJ |
| description | Escherichia coli sfa+ strains isolated from poultry were serotyped and characterized by polymerase chain reaction (PCR) and amplified fragment length polymorphism (AFLP). Isolates collected from 12 Brazilian poultry farms mostly belonged to serogroup O6, followed by serogroups O2, O8, O21, O46, O78, O88, O106, O111, and O143. Virulence genes associated were: iuc 90%, fim 86% neuS 60%, hly 34%, tsh 28%, crl/csg 26%, iss 26%, pap 18%, and 14% cnf. Strains from the same farm presented more than one genotypic pattern belonging to different profiles in AFLP. AFLP showed a clonal relation between Escherichia coli sfa+ serogroup O6. The virulence genes found in these strains reveal some similarity with extraintestinal E. coli (ExPEC), thus alerting for potential zoonotic risk. |
| format | Article |
| id | doaj-art-f6abd318f09e4f8ebf5eb066a4aa5b25 |
| institution | Kabale University |
| issn | 1537-744X |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Scientific World Journal |
| spelling | doaj-art-f6abd318f09e4f8ebf5eb066a4aa5b252025-02-03T05:45:02ZengWileyThe Scientific World Journal1537-744X2012-01-01201210.1100/2012/437342437342Prevalence of Avian Pathogenic Escherichia coli (APEC) Clone Harboring sfa Gene in BrazilTerezinha Knöbl0Andrea Micke Moreno1Renata Paixão2Tânia Aparecida Tardelli Gomes3Mônica Aparecida Midolli Vieira4Domingos da Silva Leite5Jesus E. Blanco6Antônio José Piantino Ferreira7Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo. Avenue Prof. Dr. Orlando Marques de Paiva, 87 05508-900 São Paulo, SP, BrazilFaculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo. Avenue Prof. Dr. Orlando Marques de Paiva, 87 05508-900 São Paulo, SP, BrazilFaculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo. Avenue Prof. Dr. Orlando Marques de Paiva, 87 05508-900 São Paulo, SP, BrazilDepartamento de Microbiologia, Imunologia e Parasitologia, Escola Paulista de Medicina, Universidade Federal de São Paulo, 04023-062 São Paulo, SP, BrazilFaculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo. Avenue Prof. Dr. Orlando Marques de Paiva, 87 05508-900 São Paulo, SP, BrazilInstituto de Biologia, Universidade Estadual de Campinas (UNICAMP), 13083-970, Campinas, SP, BrazilLaboratório de Referência de E. coli (LREC), Departamento de Microbioloxía e Parasitoloxía, Facultade de Veterinaria, Universidade de Santiago de Compostela (USC), 27002 Lugo, SpainFaculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo. Avenue Prof. Dr. Orlando Marques de Paiva, 87 05508-900 São Paulo, SP, BrazilEscherichia coli sfa+ strains isolated from poultry were serotyped and characterized by polymerase chain reaction (PCR) and amplified fragment length polymorphism (AFLP). Isolates collected from 12 Brazilian poultry farms mostly belonged to serogroup O6, followed by serogroups O2, O8, O21, O46, O78, O88, O106, O111, and O143. Virulence genes associated were: iuc 90%, fim 86% neuS 60%, hly 34%, tsh 28%, crl/csg 26%, iss 26%, pap 18%, and 14% cnf. Strains from the same farm presented more than one genotypic pattern belonging to different profiles in AFLP. AFLP showed a clonal relation between Escherichia coli sfa+ serogroup O6. The virulence genes found in these strains reveal some similarity with extraintestinal E. coli (ExPEC), thus alerting for potential zoonotic risk.http://dx.doi.org/10.1100/2012/437342 |
| spellingShingle | Terezinha Knöbl Andrea Micke Moreno Renata Paixão Tânia Aparecida Tardelli Gomes Mônica Aparecida Midolli Vieira Domingos da Silva Leite Jesus E. Blanco Antônio José Piantino Ferreira Prevalence of Avian Pathogenic Escherichia coli (APEC) Clone Harboring sfa Gene in Brazil The Scientific World Journal |
| title | Prevalence of Avian Pathogenic Escherichia coli (APEC) Clone Harboring sfa Gene in Brazil |
| title_full | Prevalence of Avian Pathogenic Escherichia coli (APEC) Clone Harboring sfa Gene in Brazil |
| title_fullStr | Prevalence of Avian Pathogenic Escherichia coli (APEC) Clone Harboring sfa Gene in Brazil |
| title_full_unstemmed | Prevalence of Avian Pathogenic Escherichia coli (APEC) Clone Harboring sfa Gene in Brazil |
| title_short | Prevalence of Avian Pathogenic Escherichia coli (APEC) Clone Harboring sfa Gene in Brazil |
| title_sort | prevalence of avian pathogenic escherichia coli apec clone harboring sfa gene in brazil |
| url | http://dx.doi.org/10.1100/2012/437342 |
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