Rapid screening of 420 pesticide residues in fruits and vegetables using ultra high performance liquid chromatography combined with quadrupole-time of flight mass spectrometry
A new method for screening and identification 420 pesticide residues in fruits and vegetables by ultra-performance liquid chromatography coupled with quadrupole-time of flight mass spectrometry (UPLC-Q-TOF/MS) were developed. The samples were extracted with acetonitrile/acetic acid (99:1, V/V), and...
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| Format: | Article |
| Language: | English |
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Tsinghua University Press
2023-07-01
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| Series: | Food Science and Human Wellness |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213453022002439 |
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| author | Junmei Ma Sufang Fan Liqiong Yang Liangna He Hongwen Zhai Xiaowei Ren Qiang Li Yan Zhang |
| author_facet | Junmei Ma Sufang Fan Liqiong Yang Liangna He Hongwen Zhai Xiaowei Ren Qiang Li Yan Zhang |
| author_sort | Junmei Ma |
| collection | DOAJ |
| description | A new method for screening and identification 420 pesticide residues in fruits and vegetables by ultra-performance liquid chromatography coupled with quadrupole-time of flight mass spectrometry (UPLC-Q-TOF/MS) were developed. The samples were extracted with acetonitrile/acetic acid (99:1, V/V), and clean-up by SinChERS-Nano (single-step, cheap, effective, rugged, safe, nano) column, determined by UPLC-Q-TOF/MS. The accurate mass database and MS/MS database which contains 420 pesticides were established, the automatic retrieval of detection results was carried on according to the accurate mass, retention time, isotope ratio, ion fragment information, and so on. Method verification was performed on leeks samples. The results showed that 420 pesticides had good linearity in the range of 0.1–100 μg/L, and the correlation coeffificients (R2) was greater than 0.990. The limits of detections (LODs) and limits of quantifications (LOQs) of 420 pesticides were in range of 0.05–2.0 and 0.1–5.0 μg/L, respectively. The average spike recoveries at 3 levels were 70.1 % to 119.7 %, and the relative standard deviations (RSD) were lower than 20 % (n = 6). With this method, a survey of pesticide residues was conducted for 110 samples of 10 different fruits and vegetables, which provided scientific data for ensuring pesticide residue safety of the fruits and vegetables consumed daily by the public. This method was simple, sensitive and accurate, and could be used for rapid screening of 420 pesticide residues in fruits and vegetables. |
| format | Article |
| id | doaj-art-f65476cbed274533808305e28569f80a |
| institution | Kabale University |
| issn | 2213-4530 |
| language | English |
| publishDate | 2023-07-01 |
| publisher | Tsinghua University Press |
| record_format | Article |
| series | Food Science and Human Wellness |
| spelling | doaj-art-f65476cbed274533808305e28569f80a2025-02-03T07:06:24ZengTsinghua University PressFood Science and Human Wellness2213-45302023-07-0112410641070Rapid screening of 420 pesticide residues in fruits and vegetables using ultra high performance liquid chromatography combined with quadrupole-time of flight mass spectrometryJunmei Ma0Sufang Fan1Liqiong Yang2Liangna He3Hongwen Zhai4Xiaowei Ren5Qiang Li6Yan Zhang7Hebei Food Inspection and Research Institute, Hebei Engineering Research Center for Special Food Safety and Health, Key Laboratory of Special Food Supervision Technology for State Market Regulation, Hebei Food Safety Key Laboratory, Shijiazhuang 050200, ChinaHebei Food Inspection and Research Institute, Hebei Engineering Research Center for Special Food Safety and Health, Key Laboratory of Special Food Supervision Technology for State Market Regulation, Hebei Food Safety Key Laboratory, Shijiazhuang 050200, ChinaHebei Food Inspection and Research Institute, Hebei Engineering Research Center for Special Food Safety and Health, Key Laboratory of Special Food Supervision Technology for State Market Regulation, Hebei Food Safety Key Laboratory, Shijiazhuang 050200, ChinaHebei Food Inspection and Research Institute, Hebei Engineering Research Center for Special Food Safety and Health, Key Laboratory of Special Food Supervision Technology for State Market Regulation, Hebei Food Safety Key Laboratory, Shijiazhuang 050200, ChinaHebei Food Inspection and Research Institute, Hebei Engineering Research Center for Special Food Safety and Health, Key Laboratory of Special Food Supervision Technology for State Market Regulation, Hebei Food Safety Key Laboratory, Shijiazhuang 050200, ChinaHebei Food Inspection and Research Institute, Hebei Engineering Research Center for Special Food Safety and Health, Key Laboratory of Special Food Supervision Technology for State Market Regulation, Hebei Food Safety Key Laboratory, Shijiazhuang 050200, ChinaHebei Food Inspection and Research Institute, Hebei Engineering Research Center for Special Food Safety and Health, Key Laboratory of Special Food Supervision Technology for State Market Regulation, Hebei Food Safety Key Laboratory, Shijiazhuang 050200, China; Corresponding authors at: Hebei Food Inspection and Research Institute, Hebei Engineering Research Center for Special Food Safety and Health, Key Laboratory of Special Food Supervision Technology for State Market Regulation, Hebei Food Safety Key Laboratory, Shijiazhuang 050200, ChinaHebei Food Inspection and Research Institute, Hebei Engineering Research Center for Special Food Safety and Health, Key Laboratory of Special Food Supervision Technology for State Market Regulation, Hebei Food Safety Key Laboratory, Shijiazhuang 050200, China; Hebei Key Laboratory of Forensic Medicine, College of Forensic Medicine, Hebei Medical University, Shijiazhuang 050017, China; Corresponding authors at: Hebei Food Inspection and Research Institute, Hebei Engineering Research Center for Special Food Safety and Health, Key Laboratory of Special Food Supervision Technology for State Market Regulation, Hebei Food Safety Key Laboratory, Shijiazhuang 050200, ChinaA new method for screening and identification 420 pesticide residues in fruits and vegetables by ultra-performance liquid chromatography coupled with quadrupole-time of flight mass spectrometry (UPLC-Q-TOF/MS) were developed. The samples were extracted with acetonitrile/acetic acid (99:1, V/V), and clean-up by SinChERS-Nano (single-step, cheap, effective, rugged, safe, nano) column, determined by UPLC-Q-TOF/MS. The accurate mass database and MS/MS database which contains 420 pesticides were established, the automatic retrieval of detection results was carried on according to the accurate mass, retention time, isotope ratio, ion fragment information, and so on. Method verification was performed on leeks samples. The results showed that 420 pesticides had good linearity in the range of 0.1–100 μg/L, and the correlation coeffificients (R2) was greater than 0.990. The limits of detections (LODs) and limits of quantifications (LOQs) of 420 pesticides were in range of 0.05–2.0 and 0.1–5.0 μg/L, respectively. The average spike recoveries at 3 levels were 70.1 % to 119.7 %, and the relative standard deviations (RSD) were lower than 20 % (n = 6). With this method, a survey of pesticide residues was conducted for 110 samples of 10 different fruits and vegetables, which provided scientific data for ensuring pesticide residue safety of the fruits and vegetables consumed daily by the public. This method was simple, sensitive and accurate, and could be used for rapid screening of 420 pesticide residues in fruits and vegetables.http://www.sciencedirect.com/science/article/pii/S2213453022002439UPLC-Q-TOF/MSSinChERS-Nano columnPesticide residuesFruits and vegetables |
| spellingShingle | Junmei Ma Sufang Fan Liqiong Yang Liangna He Hongwen Zhai Xiaowei Ren Qiang Li Yan Zhang Rapid screening of 420 pesticide residues in fruits and vegetables using ultra high performance liquid chromatography combined with quadrupole-time of flight mass spectrometry Food Science and Human Wellness UPLC-Q-TOF/MS SinChERS-Nano column Pesticide residues Fruits and vegetables |
| title | Rapid screening of 420 pesticide residues in fruits and vegetables using ultra high performance liquid chromatography combined with quadrupole-time of flight mass spectrometry |
| title_full | Rapid screening of 420 pesticide residues in fruits and vegetables using ultra high performance liquid chromatography combined with quadrupole-time of flight mass spectrometry |
| title_fullStr | Rapid screening of 420 pesticide residues in fruits and vegetables using ultra high performance liquid chromatography combined with quadrupole-time of flight mass spectrometry |
| title_full_unstemmed | Rapid screening of 420 pesticide residues in fruits and vegetables using ultra high performance liquid chromatography combined with quadrupole-time of flight mass spectrometry |
| title_short | Rapid screening of 420 pesticide residues in fruits and vegetables using ultra high performance liquid chromatography combined with quadrupole-time of flight mass spectrometry |
| title_sort | rapid screening of 420 pesticide residues in fruits and vegetables using ultra high performance liquid chromatography combined with quadrupole time of flight mass spectrometry |
| topic | UPLC-Q-TOF/MS SinChERS-Nano column Pesticide residues Fruits and vegetables |
| url | http://www.sciencedirect.com/science/article/pii/S2213453022002439 |
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