Transcriptome Analysis of Interspecific Hybrid between Brassica napus and B. rapa Reveals Heterosis for Oil Rape Improvement
The hybrid between Brassica napus and B. rapa displays obvious heterosis in both growth performance and stress tolerances. A comparative transcriptome analysis for B. napus (AnAnCC genome), B. rapa (ArAr genome), and its hybrid F1 (AnArC genome) was carried out to reveal the possible molecular mecha...
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| Format: | Article |
| Language: | English |
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Wiley
2015-01-01
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| Series: | International Journal of Genomics |
| Online Access: | http://dx.doi.org/10.1155/2015/230985 |
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| author | Jinfang Zhang Guangrong Li Haojie Li Xiaobin Pu Jun Jiang Liang Chai Benchuan Zheng Cheng Cui Zujun Yang Yongqing Zhu Liangcai Jiang |
| author_facet | Jinfang Zhang Guangrong Li Haojie Li Xiaobin Pu Jun Jiang Liang Chai Benchuan Zheng Cheng Cui Zujun Yang Yongqing Zhu Liangcai Jiang |
| author_sort | Jinfang Zhang |
| collection | DOAJ |
| description | The hybrid between Brassica napus and B. rapa displays obvious heterosis in both growth performance and stress tolerances. A comparative transcriptome analysis for B. napus (AnAnCC genome), B. rapa (ArAr genome), and its hybrid F1 (AnArC genome) was carried out to reveal the possible molecular mechanisms of heterosis at the gene expression level. A total of 40,320 nonredundant unigenes were identified using B. rapa (AA genome) and B. oleracea (CC genome) as reference genomes. A total of 6,816 differentially expressed genes (DEGs) were mapped in the A and C genomes with 4,946 DEGs displayed nonadditively by comparing the gene expression patterns among the three samples. The coexistence of nonadditive DEGs including high-parent dominance, low-parent dominance, overdominance, and underdominance was observed in the gene action modes of F1 hybrid, which were potentially related to the heterosis. The coexistence of multiple gene actions in the hybrid was observed and provided a list of candidate genes and pathways for heterosis. The expression bias of transposable element-associated genes was also observed in the hybrid compared to their parents. The present study could be helpful for the better understanding of the determination and regulation of mechanisms of heterosis to aid Brassica improvement. |
| format | Article |
| id | doaj-art-f640e0867c594cddbb40608f43146039 |
| institution | Kabale University |
| issn | 2314-436X 2314-4378 |
| language | English |
| publishDate | 2015-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Genomics |
| spelling | doaj-art-f640e0867c594cddbb40608f431460392025-02-03T01:32:01ZengWileyInternational Journal of Genomics2314-436X2314-43782015-01-01201510.1155/2015/230985230985Transcriptome Analysis of Interspecific Hybrid between Brassica napus and B. rapa Reveals Heterosis for Oil Rape ImprovementJinfang Zhang0Guangrong Li1Haojie Li2Xiaobin Pu3Jun Jiang4Liang Chai5Benchuan Zheng6Cheng Cui7Zujun Yang8Yongqing Zhu9Liangcai Jiang10Crop Science Institute, Sichuan Academy of Agricultural Sciences, Chengdu, Sichuan 6110066, ChinaSchool of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu, Sichuan 610054, ChinaCrop Science Institute, Sichuan Academy of Agricultural Sciences, Chengdu, Sichuan 6110066, ChinaCrop Science Institute, Sichuan Academy of Agricultural Sciences, Chengdu, Sichuan 6110066, ChinaCrop Science Institute, Sichuan Academy of Agricultural Sciences, Chengdu, Sichuan 6110066, ChinaCrop Science Institute, Sichuan Academy of Agricultural Sciences, Chengdu, Sichuan 6110066, ChinaCrop Science Institute, Sichuan Academy of Agricultural Sciences, Chengdu, Sichuan 6110066, ChinaCrop Science Institute, Sichuan Academy of Agricultural Sciences, Chengdu, Sichuan 6110066, ChinaSchool of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu, Sichuan 610054, ChinaInstitute of Agro-Products Processing Science and Technology, Sichuan Academy of Agricultural Sciences, Chengdu, Sichuan 6110066, ChinaCrop Science Institute, Sichuan Academy of Agricultural Sciences, Chengdu, Sichuan 6110066, ChinaThe hybrid between Brassica napus and B. rapa displays obvious heterosis in both growth performance and stress tolerances. A comparative transcriptome analysis for B. napus (AnAnCC genome), B. rapa (ArAr genome), and its hybrid F1 (AnArC genome) was carried out to reveal the possible molecular mechanisms of heterosis at the gene expression level. A total of 40,320 nonredundant unigenes were identified using B. rapa (AA genome) and B. oleracea (CC genome) as reference genomes. A total of 6,816 differentially expressed genes (DEGs) were mapped in the A and C genomes with 4,946 DEGs displayed nonadditively by comparing the gene expression patterns among the three samples. The coexistence of nonadditive DEGs including high-parent dominance, low-parent dominance, overdominance, and underdominance was observed in the gene action modes of F1 hybrid, which were potentially related to the heterosis. The coexistence of multiple gene actions in the hybrid was observed and provided a list of candidate genes and pathways for heterosis. The expression bias of transposable element-associated genes was also observed in the hybrid compared to their parents. The present study could be helpful for the better understanding of the determination and regulation of mechanisms of heterosis to aid Brassica improvement.http://dx.doi.org/10.1155/2015/230985 |
| spellingShingle | Jinfang Zhang Guangrong Li Haojie Li Xiaobin Pu Jun Jiang Liang Chai Benchuan Zheng Cheng Cui Zujun Yang Yongqing Zhu Liangcai Jiang Transcriptome Analysis of Interspecific Hybrid between Brassica napus and B. rapa Reveals Heterosis for Oil Rape Improvement International Journal of Genomics |
| title | Transcriptome Analysis of Interspecific Hybrid between Brassica napus and B. rapa Reveals Heterosis for Oil Rape Improvement |
| title_full | Transcriptome Analysis of Interspecific Hybrid between Brassica napus and B. rapa Reveals Heterosis for Oil Rape Improvement |
| title_fullStr | Transcriptome Analysis of Interspecific Hybrid between Brassica napus and B. rapa Reveals Heterosis for Oil Rape Improvement |
| title_full_unstemmed | Transcriptome Analysis of Interspecific Hybrid between Brassica napus and B. rapa Reveals Heterosis for Oil Rape Improvement |
| title_short | Transcriptome Analysis of Interspecific Hybrid between Brassica napus and B. rapa Reveals Heterosis for Oil Rape Improvement |
| title_sort | transcriptome analysis of interspecific hybrid between brassica napus and b rapa reveals heterosis for oil rape improvement |
| url | http://dx.doi.org/10.1155/2015/230985 |
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