Protocol used for AFLP by automatic laser fluorescence analysis
The AFLP (amplified fragment length polymorphism) technique using fluorescence-labelled primers and automated detection is described and some features of this method are discussed. The modified AFLP technique is suitable for efficient fingerprinting analysis and gives a much higher resolution than s...
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| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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University of Ljubljana Press (Založba Univerze v Ljubljani)
2003-05-01
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| Series: | Acta Agriculturae Slovenica |
| Online Access: | https://journals.uni-lj.si/aas/article/view/15323 |
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| _version_ | 1850035058163843072 |
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| author | Nataša ŠTAJNER Branka JAVORNIK Dunja BANDELJ |
| author_facet | Nataša ŠTAJNER Branka JAVORNIK Dunja BANDELJ |
| author_sort | Nataša ŠTAJNER |
| collection | DOAJ |
| description | The AFLP (amplified fragment length polymorphism) technique using fluorescence-labelled primers and automated detection is described and some features of this method are discussed. The modified AFLP technique is suitable for efficient fingerprinting analysis and gives a much higher resolution than silver staining detection systems. With the aid of an automated ALFexpress sequencer and computer software, the AFLP method is much considerably easier to perform and the total time required is reduced. |
| format | Article |
| id | doaj-art-f62ae5839a734dcaa82ae00a14d0edd0 |
| institution | DOAJ |
| issn | 1854-1941 |
| language | English |
| publishDate | 2003-05-01 |
| publisher | University of Ljubljana Press (Založba Univerze v Ljubljani) |
| record_format | Article |
| series | Acta Agriculturae Slovenica |
| spelling | doaj-art-f62ae5839a734dcaa82ae00a14d0edd02025-08-20T02:57:36ZengUniversity of Ljubljana Press (Založba Univerze v Ljubljani)Acta Agriculturae Slovenica1854-19412003-05-01811394510.14720/aas.2003.81.1.1532321716Protocol used for AFLP by automatic laser fluorescence analysisNataša ŠTAJNER0Branka JAVORNIK1Dunja BANDELJ2University of Ljubljana, Biotechnical Faculty, Jamnikarjeva 101, SI-1000 Ljubljana, SloveniaUniversity of Ljubljana, Biotechnical Faculty, Jamnikarjeva 101, SI-1000 Ljubljana, SloveniaScience and Research Centre of the Republic Slovenia, Koper, SloveniaThe AFLP (amplified fragment length polymorphism) technique using fluorescence-labelled primers and automated detection is described and some features of this method are discussed. The modified AFLP technique is suitable for efficient fingerprinting analysis and gives a much higher resolution than silver staining detection systems. With the aid of an automated ALFexpress sequencer and computer software, the AFLP method is much considerably easier to perform and the total time required is reduced.https://journals.uni-lj.si/aas/article/view/15323 |
| spellingShingle | Nataša ŠTAJNER Branka JAVORNIK Dunja BANDELJ Protocol used for AFLP by automatic laser fluorescence analysis Acta Agriculturae Slovenica |
| title | Protocol used for AFLP by automatic laser fluorescence analysis |
| title_full | Protocol used for AFLP by automatic laser fluorescence analysis |
| title_fullStr | Protocol used for AFLP by automatic laser fluorescence analysis |
| title_full_unstemmed | Protocol used for AFLP by automatic laser fluorescence analysis |
| title_short | Protocol used for AFLP by automatic laser fluorescence analysis |
| title_sort | protocol used for aflp by automatic laser fluorescence analysis |
| url | https://journals.uni-lj.si/aas/article/view/15323 |
| work_keys_str_mv | AT natasastajner protocolusedforaflpbyautomaticlaserfluorescenceanalysis AT brankajavornik protocolusedforaflpbyautomaticlaserfluorescenceanalysis AT dunjabandelj protocolusedforaflpbyautomaticlaserfluorescenceanalysis |