Immunomodulatory effect of hepatitis B virus (HBV) antigens and MRSA antigens on immune response
Abstract Background: Methicillin-resistant Staphylococcus aureus is one of the most common human invaders and causes different types of illness worldwide. Objectives: This study aimed to illustrate the immune response of purified human peripheral blood mononuclear cells against somatic antigens of m...
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2024-01-01
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| Series: | Medical Journal of Babylon |
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| Online Access: | https://doi.org/10.4103/MJBL.MJBL_1104_23 |
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| author | Entidhar A. Hadi Mohammad Abd-Kadhum Al-Saadi Kaiser N. Madlum |
| author_facet | Entidhar A. Hadi Mohammad Abd-Kadhum Al-Saadi Kaiser N. Madlum |
| author_sort | Entidhar A. Hadi |
| collection | DOAJ |
| description | Abstract Background: Methicillin-resistant Staphylococcus aureus is one of the most common human invaders and causes different types of illness worldwide. Objectives: This study aimed to illustrate the immune response of purified human peripheral blood mononuclear cells against somatic antigens of methicillin-resistant S. aureus (MRSA) and to study the immunomodulatory effect of hepatitis B virus (HBV) antigens on this immune response. Materials and Methods: A case–control study was applied on 75 peripheral blood mononuclear cells (PBMCs) samples. This work involved five experimental groups, each group involved 15 samples. Group I involves separated PBMCs (1 × 106 cells/mL) alone as a control; group II involves PBMCs stimulated with killed somatic MRSA antigen; group III of PBMCs were stimulated with HBV vaccine only; group IV involves PBMCs pretreated with HBV vaccine for 48 h, then with killed somatic MRSA antigen; and group V involves PBMCs stimulated with mixed of killed somatic MRSA antigen and HBV vaccine. The immune response against MRSA somatic antigens was assessed by measurement of interferon-γ, interleukin-4, and interleukin-5 by enzyme-linked immunosorbent assay technique. Results: The results indicated a significant increase (P < 0.05) in the mean value of IFN-γ in group II (125.9995 pg/mL) and III group (133.6504 pg/mL) and group IV (132.0271 pg/mL) as compared with control (group I) (49.6203 pg/mL). Also, the results showed no significant differences between control group I and group V (49.6203 and 9.6087 pg/mL). IL-4 and IL-5 concentration results revealed a significant increase in all treated groups as compared with control untreated group I. Conclusion: Immune response represented by regulatory cytokines against MRSA is significantly induced by somatic antigens. |
| format | Article |
| id | doaj-art-f6264dc16a2d43f6b6c06f70ec19c788 |
| institution | Kabale University |
| issn | 1812-156X 2312-6760 |
| language | English |
| publishDate | 2024-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Medical Journal of Babylon |
| spelling | doaj-art-f6264dc16a2d43f6b6c06f70ec19c7882025-01-25T10:14:51ZengWolters Kluwer Medknow PublicationsMedical Journal of Babylon1812-156X2312-67602024-01-0121122723010.4103/MJBL.MJBL_1104_23Immunomodulatory effect of hepatitis B virus (HBV) antigens and MRSA antigens on immune responseEntidhar A. HadiMohammad Abd-Kadhum Al-SaadiKaiser N. MadlumAbstract Background: Methicillin-resistant Staphylococcus aureus is one of the most common human invaders and causes different types of illness worldwide. Objectives: This study aimed to illustrate the immune response of purified human peripheral blood mononuclear cells against somatic antigens of methicillin-resistant S. aureus (MRSA) and to study the immunomodulatory effect of hepatitis B virus (HBV) antigens on this immune response. Materials and Methods: A case–control study was applied on 75 peripheral blood mononuclear cells (PBMCs) samples. This work involved five experimental groups, each group involved 15 samples. Group I involves separated PBMCs (1 × 106 cells/mL) alone as a control; group II involves PBMCs stimulated with killed somatic MRSA antigen; group III of PBMCs were stimulated with HBV vaccine only; group IV involves PBMCs pretreated with HBV vaccine for 48 h, then with killed somatic MRSA antigen; and group V involves PBMCs stimulated with mixed of killed somatic MRSA antigen and HBV vaccine. The immune response against MRSA somatic antigens was assessed by measurement of interferon-γ, interleukin-4, and interleukin-5 by enzyme-linked immunosorbent assay technique. Results: The results indicated a significant increase (P < 0.05) in the mean value of IFN-γ in group II (125.9995 pg/mL) and III group (133.6504 pg/mL) and group IV (132.0271 pg/mL) as compared with control (group I) (49.6203 pg/mL). Also, the results showed no significant differences between control group I and group V (49.6203 and 9.6087 pg/mL). IL-4 and IL-5 concentration results revealed a significant increase in all treated groups as compared with control untreated group I. Conclusion: Immune response represented by regulatory cytokines against MRSA is significantly induced by somatic antigens.https://doi.org/10.4103/MJBL.MJBL_1104_23hbv vaccineifn-γil-4il-5mrsa |
| spellingShingle | Entidhar A. Hadi Mohammad Abd-Kadhum Al-Saadi Kaiser N. Madlum Immunomodulatory effect of hepatitis B virus (HBV) antigens and MRSA antigens on immune response Medical Journal of Babylon hbv vaccine ifn-γ il-4 il-5 mrsa |
| title | Immunomodulatory effect of hepatitis B virus (HBV) antigens and MRSA antigens on immune response |
| title_full | Immunomodulatory effect of hepatitis B virus (HBV) antigens and MRSA antigens on immune response |
| title_fullStr | Immunomodulatory effect of hepatitis B virus (HBV) antigens and MRSA antigens on immune response |
| title_full_unstemmed | Immunomodulatory effect of hepatitis B virus (HBV) antigens and MRSA antigens on immune response |
| title_short | Immunomodulatory effect of hepatitis B virus (HBV) antigens and MRSA antigens on immune response |
| title_sort | immunomodulatory effect of hepatitis b virus hbv antigens and mrsa antigens on immune response |
| topic | hbv vaccine ifn-γ il-4 il-5 mrsa |
| url | https://doi.org/10.4103/MJBL.MJBL_1104_23 |
| work_keys_str_mv | AT entidharahadi immunomodulatoryeffectofhepatitisbvirushbvantigensandmrsaantigensonimmuneresponse AT mohammadabdkadhumalsaadi immunomodulatoryeffectofhepatitisbvirushbvantigensandmrsaantigensonimmuneresponse AT kaisernmadlum immunomodulatoryeffectofhepatitisbvirushbvantigensandmrsaantigensonimmuneresponse |