A proteomic analysis identifies higher AHSG (Alpha-2-HS-glycoprotein) in saliva of oropharyngeal cancer patients – A potential salivary biomarker
Objective: Oropharyngeal cancer (OPC) is one of the most prevailing cancers worldwide with squamous cell type representing the major cases. OPC has a poor survival rate which is majorly attributed to late presentation of the disease with non-availability of a suitable tumor marker as one of the majo...
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| Format: | Article |
| Language: | English |
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Elsevier
2024-06-01
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| Series: | Oral Oncology Reports |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2772906024003248 |
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| author | Anu Jain Aafaque Ahmad Khan Rajandeep Kaur Roshan K Verma Jaimanti Bakshi Aditi Chatterjee Amanjit Bal Sushmita Ghoshal Arnab Pal |
| author_facet | Anu Jain Aafaque Ahmad Khan Rajandeep Kaur Roshan K Verma Jaimanti Bakshi Aditi Chatterjee Amanjit Bal Sushmita Ghoshal Arnab Pal |
| author_sort | Anu Jain |
| collection | DOAJ |
| description | Objective: Oropharyngeal cancer (OPC) is one of the most prevailing cancers worldwide with squamous cell type representing the major cases. OPC has a poor survival rate which is majorly attributed to late presentation of the disease with non-availability of a suitable tumor marker as one of the major reasons associated. This study was designed to identify a potential salivary biomarker for OPC using proteomics approach. Materials and methods: OPC cases were enrolled in different cohorts depending on the tobacco consumption habits as smokers, chewers, smokers and chewers and no tobacco consumers. Healthy controls without any tobacco consumption habits were enrolled simultaneously. Tandem Mass Tag based relative quantification was done to identify the cancer related dysregulated proteins. Dysregulated proteins were validated further in a larger cohort of cases and controls. Furthermore, the tissue expression of AHSG was analysed using the TCGA data available on cBioportal and GEPIA2. Results: 171 proteins were identified of which 135 proteins were dysregulated with ≥2-fold change. Differential expression of proteins in different OPC study cohorts was observed. 15 candidate proteins were selected for validation after analysing the data. AHSG (alpha-2HS-glycoprotein) was one of the candidates, validated in a separate larger cohort of cases and controls and was found to be significantly elevated in cases. AHSG was also found to be upregulated in the tumor tissue of HNSCC patients and patients with low AHSG were found to have the better overall survival outcome in comparison to the cases with higher AHSG. Conclusions: AHSG levels are elevated in saliva of OPC cases compared to non-OPC healthy controls. This expression pattern of AHSG should be evaluated further as a potential biomarker for OPC. |
| format | Article |
| id | doaj-art-f5fe072f101a49b3a753355886d3d1ec |
| institution | Kabale University |
| issn | 2772-9060 |
| language | English |
| publishDate | 2024-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Oral Oncology Reports |
| spelling | doaj-art-f5fe072f101a49b3a753355886d3d1ec2025-01-09T06:16:27ZengElsevierOral Oncology Reports2772-90602024-06-0110100478A proteomic analysis identifies higher AHSG (Alpha-2-HS-glycoprotein) in saliva of oropharyngeal cancer patients – A potential salivary biomarkerAnu Jain0Aafaque Ahmad Khan1Rajandeep Kaur2Roshan K Verma3Jaimanti Bakshi4Aditi Chatterjee5Amanjit Bal6Sushmita Ghoshal7Arnab Pal8Department of Biochemistry, Postgraduate Institute of Medical Education and Research, Chandigarh, 160012, IndiaInstitute of Bioinformatics, International Tech Park, Whitefield, Bangalore, Karnataka, 560066, IndiaDepartment of Biochemistry, Postgraduate Institute of Medical Education and Research, Chandigarh, 160012, IndiaDepartment of Otolaryngology, Postgraduate Institute of Medical Education and Research, Chandigarh, 160012, IndiaDepartment of Otolaryngology, Postgraduate Institute of Medical Education and Research, Chandigarh, 160012, IndiaInstitute of Bioinformatics, International Tech Park, Whitefield, Bangalore, Karnataka, 560066, India; Manipal Academy of Higher Education (MAHE), Manipal, IndiaDepartment of Histopathology, Postgraduate Institute of Medical Education and Research, Chandigarh, 160012, IndiaDepartment of Radiotherapy, Postgraduate Institute of Medical Education and Research, Chandigarh, 160012, IndiaDepartment of Biochemistry, Postgraduate Institute of Medical Education and Research, Chandigarh, 160012, India; Corresponding author. Department of Biochemistry, Post Graduate Institute of Medical Education and Research, Chandigarh, 160012, India.Objective: Oropharyngeal cancer (OPC) is one of the most prevailing cancers worldwide with squamous cell type representing the major cases. OPC has a poor survival rate which is majorly attributed to late presentation of the disease with non-availability of a suitable tumor marker as one of the major reasons associated. This study was designed to identify a potential salivary biomarker for OPC using proteomics approach. Materials and methods: OPC cases were enrolled in different cohorts depending on the tobacco consumption habits as smokers, chewers, smokers and chewers and no tobacco consumers. Healthy controls without any tobacco consumption habits were enrolled simultaneously. Tandem Mass Tag based relative quantification was done to identify the cancer related dysregulated proteins. Dysregulated proteins were validated further in a larger cohort of cases and controls. Furthermore, the tissue expression of AHSG was analysed using the TCGA data available on cBioportal and GEPIA2. Results: 171 proteins were identified of which 135 proteins were dysregulated with ≥2-fold change. Differential expression of proteins in different OPC study cohorts was observed. 15 candidate proteins were selected for validation after analysing the data. AHSG (alpha-2HS-glycoprotein) was one of the candidates, validated in a separate larger cohort of cases and controls and was found to be significantly elevated in cases. AHSG was also found to be upregulated in the tumor tissue of HNSCC patients and patients with low AHSG were found to have the better overall survival outcome in comparison to the cases with higher AHSG. Conclusions: AHSG levels are elevated in saliva of OPC cases compared to non-OPC healthy controls. This expression pattern of AHSG should be evaluated further as a potential biomarker for OPC.http://www.sciencedirect.com/science/article/pii/S2772906024003248 |
| spellingShingle | Anu Jain Aafaque Ahmad Khan Rajandeep Kaur Roshan K Verma Jaimanti Bakshi Aditi Chatterjee Amanjit Bal Sushmita Ghoshal Arnab Pal A proteomic analysis identifies higher AHSG (Alpha-2-HS-glycoprotein) in saliva of oropharyngeal cancer patients – A potential salivary biomarker Oral Oncology Reports |
| title | A proteomic analysis identifies higher AHSG (Alpha-2-HS-glycoprotein) in saliva of oropharyngeal cancer patients – A potential salivary biomarker |
| title_full | A proteomic analysis identifies higher AHSG (Alpha-2-HS-glycoprotein) in saliva of oropharyngeal cancer patients – A potential salivary biomarker |
| title_fullStr | A proteomic analysis identifies higher AHSG (Alpha-2-HS-glycoprotein) in saliva of oropharyngeal cancer patients – A potential salivary biomarker |
| title_full_unstemmed | A proteomic analysis identifies higher AHSG (Alpha-2-HS-glycoprotein) in saliva of oropharyngeal cancer patients – A potential salivary biomarker |
| title_short | A proteomic analysis identifies higher AHSG (Alpha-2-HS-glycoprotein) in saliva of oropharyngeal cancer patients – A potential salivary biomarker |
| title_sort | proteomic analysis identifies higher ahsg alpha 2 hs glycoprotein in saliva of oropharyngeal cancer patients a potential salivary biomarker |
| url | http://www.sciencedirect.com/science/article/pii/S2772906024003248 |
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