Genetic differentiation between wildtype and spotted swamp buffaloes (Bubalus bubalis) of Indonesia based on ISSR and RAPD markers
The swamp buffaloes (Bubalus bubalis) are an important livestock that is used for draught, meat production, and cultural tradition ceremonies in Indonesia. This study aimed to characterize the wildtype and spotted buffaloes based on inter-simple sequence repeat (ISSR) and random amplified polymorphi...
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| Format: | Article |
| Language: | Arabic |
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University of Mosul, College of Veterinary Medicine
2025-07-01
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| Series: | Iraqi Journal of Veterinary Sciences |
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| Online Access: | https://www.vetmedmosul.com/article_188378_bcb8446dd7de9953ea0e695cca1822ed.pdf |
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| author | Widya Putra Salah Faraj Novi Adiningsih Abdullah Baharun Hartati Hartati Aprisal Aprisal Tulus Maulana Syahruddin Said Hasbi Hasbi Petra Sitanggang |
| author_facet | Widya Putra Salah Faraj Novi Adiningsih Abdullah Baharun Hartati Hartati Aprisal Aprisal Tulus Maulana Syahruddin Said Hasbi Hasbi Petra Sitanggang |
| author_sort | Widya Putra |
| collection | DOAJ |
| description | The swamp buffaloes (Bubalus bubalis) are an important livestock that is used for draught, meat production, and cultural tradition ceremonies in Indonesia. This study aimed to characterize the wildtype and spotted buffaloes based on inter-simple sequence repeat (ISSR) and random amplified polymorphic DNA (RAPD) markers. A total of twenty-two buffaloes (13 wildtype and nine spotted) were used for the experimental animals. Two ISSR primers (P01, P02) and three RAPD primers (SRILLS-2, SRILLS-5, SRILLS-6) were assessed to discriminate between the two types of buffalo. Results showed that the ISSR primers had a higher polymorphic informative content (PIC) value than RAPD primers. Therefore, P01 had the highest polymorphic informative content (PIC) value (0.412) and SRILLS-5 as the lowest (0.063). Shannon’s diversity index (I) value in both techniques was>1.00 and indicating a various fragment sizes resulted from each primer. However, the RAPD technique can discriminate between two types of buffalo better than the ISSR technique. In the pool animals, haplotype one of SRILLS-6 (16 heads) was more frequent than other haplotypes and followed by haplotype two of P02 (14 heads). Despite this, two types of swamp buffalo in the present study can also be discriminated with a combination of ISSR and RAPD techniques accurately. In conclusion, the ISSR and RAPD primers in the present study can be used as genetic markers to differentiate swamp buffaloes of Indonesia. |
| format | Article |
| id | doaj-art-f5ee9e94681640fb91edcd720c0fcffc |
| institution | DOAJ |
| issn | 1607-3894 2071-1255 |
| language | Arabic |
| publishDate | 2025-07-01 |
| publisher | University of Mosul, College of Veterinary Medicine |
| record_format | Article |
| series | Iraqi Journal of Veterinary Sciences |
| spelling | doaj-art-f5ee9e94681640fb91edcd720c0fcffc2025-08-20T02:39:40ZaraUniversity of Mosul, College of Veterinary MedicineIraqi Journal of Veterinary Sciences1607-38942071-12552025-07-0139360961510.33899/ijvs.2025.157117.4107188378Genetic differentiation between wildtype and spotted swamp buffaloes (Bubalus bubalis) of Indonesia based on ISSR and RAPD markersWidya Putra0Salah Faraj1Novi Adiningsih2Abdullah Baharun3Hartati Hartati4Aprisal Aprisal5Tulus Maulana6Syahruddin Said7Hasbi Hasbi8Petra Sitanggang9National Research and Innovation Agency, Bogor-Jakarta Rd. Km. 46 CibinongDepartment of Biology, College of Science, Misan UniversityDepartment of Animal Husbandry, Faculty of Agriculture, Djuanda UniversityDepartment of Animal Husbandry, Faculty of Agriculture, Djuanda UniversityResearch Center for Animal Husbandry, National Research and Innovation AgencyDepartment of Animal Husbandry and Veterinary of West Sumatera ProvinceResearch Center for Applied Zoology, National Research and Innovation AgencyResearch Center for Applied Zoology, National Research and Innovation AgencyDepartment of Animal Production, Faculty of Animal Science, Hasanuddin UniversityDepartment of Agrotechnology, Faculty of Agriculture, University of PapuaThe swamp buffaloes (Bubalus bubalis) are an important livestock that is used for draught, meat production, and cultural tradition ceremonies in Indonesia. This study aimed to characterize the wildtype and spotted buffaloes based on inter-simple sequence repeat (ISSR) and random amplified polymorphic DNA (RAPD) markers. A total of twenty-two buffaloes (13 wildtype and nine spotted) were used for the experimental animals. Two ISSR primers (P01, P02) and three RAPD primers (SRILLS-2, SRILLS-5, SRILLS-6) were assessed to discriminate between the two types of buffalo. Results showed that the ISSR primers had a higher polymorphic informative content (PIC) value than RAPD primers. Therefore, P01 had the highest polymorphic informative content (PIC) value (0.412) and SRILLS-5 as the lowest (0.063). Shannon’s diversity index (I) value in both techniques was>1.00 and indicating a various fragment sizes resulted from each primer. However, the RAPD technique can discriminate between two types of buffalo better than the ISSR technique. In the pool animals, haplotype one of SRILLS-6 (16 heads) was more frequent than other haplotypes and followed by haplotype two of P02 (14 heads). Despite this, two types of swamp buffalo in the present study can also be discriminated with a combination of ISSR and RAPD techniques accurately. In conclusion, the ISSR and RAPD primers in the present study can be used as genetic markers to differentiate swamp buffaloes of Indonesia.https://www.vetmedmosul.com/article_188378_bcb8446dd7de9953ea0e695cca1822ed.pdfbuffaloissrrapdspottedwildtype |
| spellingShingle | Widya Putra Salah Faraj Novi Adiningsih Abdullah Baharun Hartati Hartati Aprisal Aprisal Tulus Maulana Syahruddin Said Hasbi Hasbi Petra Sitanggang Genetic differentiation between wildtype and spotted swamp buffaloes (Bubalus bubalis) of Indonesia based on ISSR and RAPD markers Iraqi Journal of Veterinary Sciences buffalo issr rapd spotted wildtype |
| title | Genetic differentiation between wildtype and spotted swamp buffaloes (Bubalus bubalis) of Indonesia based on ISSR and RAPD markers |
| title_full | Genetic differentiation between wildtype and spotted swamp buffaloes (Bubalus bubalis) of Indonesia based on ISSR and RAPD markers |
| title_fullStr | Genetic differentiation between wildtype and spotted swamp buffaloes (Bubalus bubalis) of Indonesia based on ISSR and RAPD markers |
| title_full_unstemmed | Genetic differentiation between wildtype and spotted swamp buffaloes (Bubalus bubalis) of Indonesia based on ISSR and RAPD markers |
| title_short | Genetic differentiation between wildtype and spotted swamp buffaloes (Bubalus bubalis) of Indonesia based on ISSR and RAPD markers |
| title_sort | genetic differentiation between wildtype and spotted swamp buffaloes bubalus bubalis of indonesia based on issr and rapd markers |
| topic | buffalo issr rapd spotted wildtype |
| url | https://www.vetmedmosul.com/article_188378_bcb8446dd7de9953ea0e695cca1822ed.pdf |
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