Population genetic analysis of clinical Mycobacterium abscessus complex strains in China
BackgroundTo explore the genetic characteristics of the Mycobacterium abscessus complex (MABC) population in China, given its rising clinical importance among nontuberculous mycobacteria.MethodsWe conducted population genetic analyses on 360 MABC genomes from China, focusing on core genome multilocu...
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Frontiers Media S.A.
2025-01-01
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| Series: | Frontiers in Cellular and Infection Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fcimb.2024.1496896/full |
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| author | Xiangchen Li Xiangchen Li Yelei Zhu Yewei Lu Kunyang Wu Yang Che Xiaomeng Wang Weixin Wang Junli Gao Junshun Gao Zhengwei Liu Zhengwei Liu Zhuxian Zhou |
| author_facet | Xiangchen Li Xiangchen Li Yelei Zhu Yewei Lu Kunyang Wu Yang Che Xiaomeng Wang Weixin Wang Junli Gao Junshun Gao Zhengwei Liu Zhengwei Liu Zhuxian Zhou |
| author_sort | Xiangchen Li |
| collection | DOAJ |
| description | BackgroundTo explore the genetic characteristics of the Mycobacterium abscessus complex (MABC) population in China, given its rising clinical importance among nontuberculous mycobacteria.MethodsWe conducted population genetic analyses on 360 MABC genomes from China, focusing on core genome multilocus sequence typing (cgMLST), pan-genome characterization, population genetics, and antimicrobial resistance gene profiling.ResultsOur analysis identified 273 M. abscessus subsp. abscessus (MabA) and 87 M. abscessus subsp. massiliense (MabM) isolates, uncovering 68 sequence types (STs), with ST5 being the most common. cgMLST classified 33.3% of isolates into six dominant circulating clones (DCCs) and 49.4% into 59 genomic clusters at a threshold of 25 different alleles, including 18 international clusters linking Chinese isolates with seven other countries. The MABC pan-genome is open, with MabA exhibiting greater accessory gene diversity and higher gene turnover compared to MabM. Mobile genetic elements (MGEs), such as prophages and genomic islands, were prevalent across all genomes. 139 to 151 virulence factors (VFs) were identified per genome, with distinct accessory VFs in MabA and MabM affecting immune modulation and metabolism. Resistance gene profiling revealed ubiquitous mtrA, RbpA, and blaMAB, with MabA-specific erm(41) conferring resistance to macrolides and β-lactams. Common rrs and rrl gene mutations indicated widespread resistance to aminoglycosides and macrolides, while gyrA mutations suggested emerging fluoroquinolone resistance. An acquired erm(46) gene, likely obtained via phage-mediated horizontal gene transfer, was detected in one MabA strain.ConclusionThis study provides key genetic insights into the dynamics of MABC in China. The widespread distribution of DCCs, high genomic clustering rates, open pan-genome, and distinct resistance patterns between MabA and MabM, along with MGEs, highlight the need for targeted surveillance and tailored therapies to address emerging challenges in MABC infections. |
| format | Article |
| id | doaj-art-f5e5476dc5e7468485403f544d59fa66 |
| institution | Kabale University |
| issn | 2235-2988 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Cellular and Infection Microbiology |
| spelling | doaj-art-f5e5476dc5e7468485403f544d59fa662025-01-20T05:23:38ZengFrontiers Media S.A.Frontiers in Cellular and Infection Microbiology2235-29882025-01-011410.3389/fcimb.2024.14968961496896Population genetic analysis of clinical Mycobacterium abscessus complex strains in ChinaXiangchen Li0Xiangchen Li1Yelei Zhu2Yewei Lu3Kunyang Wu4Yang Che5Xiaomeng Wang6Weixin Wang7Junli Gao8Junshun Gao9Zhengwei Liu10Zhengwei Liu11Zhuxian Zhou12College of Chemical and Biological Engineering, Zhejiang University, Hangzhou, Zhejiang, ChinaShaoxing Key Laboratory of Infectious Diseases, Affiliated Hospital of Shaoxing University, Shaoxing, Zhejiang, ChinaThe Institute of TB Control, Zhejiang Provincial Center for Disease Control and Prevention, Hangzhou, Zhejiang, ChinaShaoxing Key Laboratory of Infectious Diseases, Affiliated Hospital of Shaoxing University, Shaoxing, Zhejiang, ChinaThe Institute of TB Control, Zhejiang Provincial Center for Disease Control and Prevention, Hangzhou, Zhejiang, ChinaInstitute of Tuberculosis Prevention and Control, Ningbo Municipal Center for Disease Control and Prevention, Ningbo, Zhejiang, ChinaThe Institute of TB Control, Zhejiang Provincial Center for Disease Control and Prevention, Hangzhou, Zhejiang, ChinaShaoxing Key Laboratory of Infectious Diseases, Affiliated Hospital of Shaoxing University, Shaoxing, Zhejiang, ChinaShaoxing Key Laboratory of Infectious Diseases, Affiliated Hospital of Shaoxing University, Shaoxing, Zhejiang, ChinaShaoxing Key Laboratory of Infectious Diseases, Affiliated Hospital of Shaoxing University, Shaoxing, Zhejiang, ChinaThe Institute of TB Control, Zhejiang Provincial Center for Disease Control and Prevention, Hangzhou, Zhejiang, ChinaKey Laboratory of Vaccine, Prevention and Control of Infectious Disease of Zhejiang Province, Zhejiang Provincial Center for Disease Control and Prevention, Hangzhou, Zhejiang, ChinaCollege of Chemical and Biological Engineering, Zhejiang University, Hangzhou, Zhejiang, ChinaBackgroundTo explore the genetic characteristics of the Mycobacterium abscessus complex (MABC) population in China, given its rising clinical importance among nontuberculous mycobacteria.MethodsWe conducted population genetic analyses on 360 MABC genomes from China, focusing on core genome multilocus sequence typing (cgMLST), pan-genome characterization, population genetics, and antimicrobial resistance gene profiling.ResultsOur analysis identified 273 M. abscessus subsp. abscessus (MabA) and 87 M. abscessus subsp. massiliense (MabM) isolates, uncovering 68 sequence types (STs), with ST5 being the most common. cgMLST classified 33.3% of isolates into six dominant circulating clones (DCCs) and 49.4% into 59 genomic clusters at a threshold of 25 different alleles, including 18 international clusters linking Chinese isolates with seven other countries. The MABC pan-genome is open, with MabA exhibiting greater accessory gene diversity and higher gene turnover compared to MabM. Mobile genetic elements (MGEs), such as prophages and genomic islands, were prevalent across all genomes. 139 to 151 virulence factors (VFs) were identified per genome, with distinct accessory VFs in MabA and MabM affecting immune modulation and metabolism. Resistance gene profiling revealed ubiquitous mtrA, RbpA, and blaMAB, with MabA-specific erm(41) conferring resistance to macrolides and β-lactams. Common rrs and rrl gene mutations indicated widespread resistance to aminoglycosides and macrolides, while gyrA mutations suggested emerging fluoroquinolone resistance. An acquired erm(46) gene, likely obtained via phage-mediated horizontal gene transfer, was detected in one MabA strain.ConclusionThis study provides key genetic insights into the dynamics of MABC in China. The widespread distribution of DCCs, high genomic clustering rates, open pan-genome, and distinct resistance patterns between MabA and MabM, along with MGEs, highlight the need for targeted surveillance and tailored therapies to address emerging challenges in MABC infections.https://www.frontiersin.org/articles/10.3389/fcimb.2024.1496896/fullmycobacterium abscessus complexpopulation Geneticsmolecular epidemiologycgMLSTpan-genome |
| spellingShingle | Xiangchen Li Xiangchen Li Yelei Zhu Yewei Lu Kunyang Wu Yang Che Xiaomeng Wang Weixin Wang Junli Gao Junshun Gao Zhengwei Liu Zhengwei Liu Zhuxian Zhou Population genetic analysis of clinical Mycobacterium abscessus complex strains in China Frontiers in Cellular and Infection Microbiology mycobacterium abscessus complex population Genetics molecular epidemiology cgMLST pan-genome |
| title | Population genetic analysis of clinical Mycobacterium abscessus complex strains in China |
| title_full | Population genetic analysis of clinical Mycobacterium abscessus complex strains in China |
| title_fullStr | Population genetic analysis of clinical Mycobacterium abscessus complex strains in China |
| title_full_unstemmed | Population genetic analysis of clinical Mycobacterium abscessus complex strains in China |
| title_short | Population genetic analysis of clinical Mycobacterium abscessus complex strains in China |
| title_sort | population genetic analysis of clinical mycobacterium abscessus complex strains in china |
| topic | mycobacterium abscessus complex population Genetics molecular epidemiology cgMLST pan-genome |
| url | https://www.frontiersin.org/articles/10.3389/fcimb.2024.1496896/full |
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