Researches on mutation breeding and fermentation conditions of oleaginous yeast

The UV-induced mutant strain UV5, obtained by UV-induced mutagenesis of Lipomyces tetrasporus NRRL Y11562, showed the excellent capability of accumulating lipid. Several factors influencing lipid production of UV5 were studied. The lipid productivity reached 9.09 g·L<sup>-1</sup> under t...

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Main Authors: ZHANG Jia, SHEN Dan-hong, ZHENG Xiao-dong
Format: Article
Language:English
Published: Zhejiang University Press 2009-09-01
Series:浙江大学学报. 农业与生命科学版
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Online Access:https://www.academax.com/doi/10.3785/j.issn.1008-9209.2009.05.004
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author ZHANG Jia
SHEN Dan-hong
ZHENG Xiao-dong
author_facet ZHANG Jia
SHEN Dan-hong
ZHENG Xiao-dong
author_sort ZHANG Jia
collection DOAJ
description The UV-induced mutant strain UV5, obtained by UV-induced mutagenesis of Lipomyces tetrasporus NRRL Y11562, showed the excellent capability of accumulating lipid. Several factors influencing lipid production of UV5 were studied. The lipid productivity reached 9.09 g·L<sup>-1</sup> under the following conditions: shaking flask fermentation, glucose as carbon source, (NH<sub>4</sub>)<sub>2</sub>SO<sub>4</sub> as nitrogen source, ratio of carbon to nitrogen at 308, initial pH 6.5, inoculation proportion of 10%, incubated at 28 ℃ for 96 h. Fatty acid compositions of the produced oil were analyzed by gas chromatography, showing the primary compositions of palmitic acid (C16:0), palmitoleic acid (C16:1), steric acid (C18:0), oleic acid (C18:1) and linoleic acid (C18:2n—1), and these compositions were similar to the vegetable oil.
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series 浙江大学学报. 农业与生命科学版
spelling doaj-art-f5d417ed90664e33b0b4e0c376e17fc42025-08-20T03:34:20ZengZhejiang University Press浙江大学学报. 农业与生命科学版1008-92092097-51552009-09-013549750210.3785/j.issn.1008-9209.2009.05.00410089209Researches on mutation breeding and fermentation conditions of oleaginous yeastZHANG JiaSHEN Dan-hongZHENG Xiao-dongThe UV-induced mutant strain UV5, obtained by UV-induced mutagenesis of Lipomyces tetrasporus NRRL Y11562, showed the excellent capability of accumulating lipid. Several factors influencing lipid production of UV5 were studied. The lipid productivity reached 9.09 g·L<sup>-1</sup> under the following conditions: shaking flask fermentation, glucose as carbon source, (NH<sub>4</sub>)<sub>2</sub>SO<sub>4</sub> as nitrogen source, ratio of carbon to nitrogen at 308, initial pH 6.5, inoculation proportion of 10%, incubated at 28 ℃ for 96 h. Fatty acid compositions of the produced oil were analyzed by gas chromatography, showing the primary compositions of palmitic acid (C16:0), palmitoleic acid (C16:1), steric acid (C18:0), oleic acid (C18:1) and linoleic acid (C18:2n—1), and these compositions were similar to the vegetable oil.https://www.academax.com/doi/10.3785/j.issn.1008-9209.2009.05.004oleaginous yeastfermentation conditionlipid composition and content
spellingShingle ZHANG Jia
SHEN Dan-hong
ZHENG Xiao-dong
Researches on mutation breeding and fermentation conditions of oleaginous yeast
浙江大学学报. 农业与生命科学版
oleaginous yeast
fermentation condition
lipid composition and content
title Researches on mutation breeding and fermentation conditions of oleaginous yeast
title_full Researches on mutation breeding and fermentation conditions of oleaginous yeast
title_fullStr Researches on mutation breeding and fermentation conditions of oleaginous yeast
title_full_unstemmed Researches on mutation breeding and fermentation conditions of oleaginous yeast
title_short Researches on mutation breeding and fermentation conditions of oleaginous yeast
title_sort researches on mutation breeding and fermentation conditions of oleaginous yeast
topic oleaginous yeast
fermentation condition
lipid composition and content
url https://www.academax.com/doi/10.3785/j.issn.1008-9209.2009.05.004
work_keys_str_mv AT zhangjia researchesonmutationbreedingandfermentationconditionsofoleaginousyeast
AT shendanhong researchesonmutationbreedingandfermentationconditionsofoleaginousyeast
AT zhengxiaodong researchesonmutationbreedingandfermentationconditionsofoleaginousyeast