Researches on mutation breeding and fermentation conditions of oleaginous yeast
The UV-induced mutant strain UV5, obtained by UV-induced mutagenesis of Lipomyces tetrasporus NRRL Y11562, showed the excellent capability of accumulating lipid. Several factors influencing lipid production of UV5 were studied. The lipid productivity reached 9.09 g·L<sup>-1</sup> under t...
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| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
Zhejiang University Press
2009-09-01
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| Series: | 浙江大学学报. 农业与生命科学版 |
| Subjects: | |
| Online Access: | https://www.academax.com/doi/10.3785/j.issn.1008-9209.2009.05.004 |
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| Summary: | The UV-induced mutant strain UV5, obtained by UV-induced mutagenesis of Lipomyces tetrasporus NRRL Y11562, showed the excellent capability of accumulating lipid. Several factors influencing lipid production of UV5 were studied. The lipid productivity reached 9.09 g·L<sup>-1</sup> under the following conditions: shaking flask fermentation, glucose as carbon source, (NH<sub>4</sub>)<sub>2</sub>SO<sub>4</sub> as nitrogen source, ratio of carbon to nitrogen at 308, initial pH 6.5, inoculation proportion of 10%, incubated at 28 ℃ for 96 h. Fatty acid compositions of the produced oil were analyzed by gas chromatography, showing the primary compositions of palmitic acid (C16:0), palmitoleic acid (C16:1), steric acid (C18:0), oleic acid (C18:1) and linoleic acid (C18:2n—1), and these compositions were similar to the vegetable oil. |
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| ISSN: | 1008-9209 2097-5155 |