Antibacterial and cytotoxic properties of esterified α- lactalbumin and β-lactoglobulin
[Formula: see text]-Lactalbumin ([Formula: see text]-La) or [Formula: see text]-lactoglobulin ([Formula: see text]-Lg) were esterified with methanol. Native proteins and their methylated esters (Est. [Formula: see text]-La and Est. [Formula: see text]-Lg) were tested for their antibacterial and cyto...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2024-12-01
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| Series: | Journal of Taibah University for Science |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/16583655.2024.2420375 |
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| author | Hossam S. El-Beltagi Eman Ibrahem Hayfa Habes Almutairi Hoda A. S. Farag Wael F. Shehata Hefnawy Mansour Mahmoud Sitohy Ali Osman |
| author_facet | Hossam S. El-Beltagi Eman Ibrahem Hayfa Habes Almutairi Hoda A. S. Farag Wael F. Shehata Hefnawy Mansour Mahmoud Sitohy Ali Osman |
| author_sort | Hossam S. El-Beltagi |
| collection | DOAJ |
| description | [Formula: see text]-Lactalbumin ([Formula: see text]-La) or [Formula: see text]-lactoglobulin ([Formula: see text]-Lg) were esterified with methanol. Native proteins and their methylated esters (Est. [Formula: see text]-La and Est. [Formula: see text]-Lg) were tested for their antibacterial and cytotoxic properties. Esterification extents for Est. [Formula: see text]-La and Est. [Formula: see text]-Lg were 92% and 88%, with isoelectric points at pH 8 and 10, respectively. The Est. [Formula: see text]-La and Est. [Formula: see text]-Lg were more inhibiting the growth of pathogenic bacteria compared to the corresponding native proteins. The impact of esterified [Formula: see text]-La and [Formula: see text]-Lg on MCF-7, A549, and Caco-2 cell lines was assessed and revealed that the concentration inhibiting 50% cellular growth (IC50) for Est. [Formula: see text]-La recorded 107.93 µg/mL against MCF-7, 616.44 µg/mL against A-549, and 388.23 µg/mL against Caco2. In parallel, Est. [Formula: see text]-Lg achieved IC50 at 101.35, 420.84, and 234.16 µg/mL, against MCF-7, A-549, and Caco2, respectively. Esterified proteins may serve as therapeutic antimicrobial agents against pathogenic bacteria and cytotoxic agents against specific cancer cells. |
| format | Article |
| id | doaj-art-f587be4ff9984a02b23e5cba436d7249 |
| institution | OA Journals |
| issn | 1658-3655 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Journal of Taibah University for Science |
| spelling | doaj-art-f587be4ff9984a02b23e5cba436d72492025-08-20T02:36:44ZengTaylor & Francis GroupJournal of Taibah University for Science1658-36552024-12-0118110.1080/16583655.2024.2420375Antibacterial and cytotoxic properties of esterified α- lactalbumin and β-lactoglobulinHossam S. El-Beltagi0Eman Ibrahem1Hayfa Habes Almutairi2Hoda A. S. Farag3Wael F. Shehata4Hefnawy Mansour5Mahmoud Sitohy6Ali Osman7Agricultural Biotechnology Department, College of Agriculture and Food Sciences, King Faisal University, Al-Ahsa, Saudi ArabiaFaculty of Agriculture, Zagazig University, Zagazig, EgyptDepartment of Chemistry, College of Science, King Faisal University, Al-Ahsa, Saudi ArabiaFood and Nutrition Department, College of Agriculture and Food Sciences, King Faisal University, Al-Ahsa, Saudi ArabiaAgricultural Biotechnology Department, College of Agriculture and Food Sciences, King Faisal University, Al-Ahsa, Saudi ArabiaFaculty of Agriculture, Zagazig University, Zagazig, EgyptFaculty of Agriculture, Zagazig University, Zagazig, EgyptFaculty of Agriculture, Zagazig University, Zagazig, Egypt[Formula: see text]-Lactalbumin ([Formula: see text]-La) or [Formula: see text]-lactoglobulin ([Formula: see text]-Lg) were esterified with methanol. Native proteins and their methylated esters (Est. [Formula: see text]-La and Est. [Formula: see text]-Lg) were tested for their antibacterial and cytotoxic properties. Esterification extents for Est. [Formula: see text]-La and Est. [Formula: see text]-Lg were 92% and 88%, with isoelectric points at pH 8 and 10, respectively. The Est. [Formula: see text]-La and Est. [Formula: see text]-Lg were more inhibiting the growth of pathogenic bacteria compared to the corresponding native proteins. The impact of esterified [Formula: see text]-La and [Formula: see text]-Lg on MCF-7, A549, and Caco-2 cell lines was assessed and revealed that the concentration inhibiting 50% cellular growth (IC50) for Est. [Formula: see text]-La recorded 107.93 µg/mL against MCF-7, 616.44 µg/mL against A-549, and 388.23 µg/mL against Caco2. In parallel, Est. [Formula: see text]-Lg achieved IC50 at 101.35, 420.84, and 234.16 µg/mL, against MCF-7, A-549, and Caco2, respectively. Esterified proteins may serve as therapeutic antimicrobial agents against pathogenic bacteria and cytotoxic agents against specific cancer cells.https://www.tandfonline.com/doi/10.1080/16583655.2024.2420375Whey proteinsPathogenic bacteriaCationic proteinsAntibacterialcytotoxic properties |
| spellingShingle | Hossam S. El-Beltagi Eman Ibrahem Hayfa Habes Almutairi Hoda A. S. Farag Wael F. Shehata Hefnawy Mansour Mahmoud Sitohy Ali Osman Antibacterial and cytotoxic properties of esterified α- lactalbumin and β-lactoglobulin Journal of Taibah University for Science Whey proteins Pathogenic bacteria Cationic proteins Antibacterial cytotoxic properties |
| title | Antibacterial and cytotoxic properties of esterified α- lactalbumin and β-lactoglobulin |
| title_full | Antibacterial and cytotoxic properties of esterified α- lactalbumin and β-lactoglobulin |
| title_fullStr | Antibacterial and cytotoxic properties of esterified α- lactalbumin and β-lactoglobulin |
| title_full_unstemmed | Antibacterial and cytotoxic properties of esterified α- lactalbumin and β-lactoglobulin |
| title_short | Antibacterial and cytotoxic properties of esterified α- lactalbumin and β-lactoglobulin |
| title_sort | antibacterial and cytotoxic properties of esterified α lactalbumin and β lactoglobulin |
| topic | Whey proteins Pathogenic bacteria Cationic proteins Antibacterial cytotoxic properties |
| url | https://www.tandfonline.com/doi/10.1080/16583655.2024.2420375 |
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