Screening of Solvent Systems for Countercurrent Chromatography Separation of Polar Constituents from <i>Ginkgo biloba</i> L. Seeds
The separation of large polar constituents presents a substantial challenge in natural product research when employing column chromatography techniques, as the process is both complex and time-consuming. In this study, an acetonitrile/tetrahydrofuran/di-(2-ethylhexyl) phosphoric acid/aqueous saturat...
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| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2025-01-01
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| Series: | Molecules |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1420-3049/30/2/409 |
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| Summary: | The separation of large polar constituents presents a substantial challenge in natural product research when employing column chromatography techniques, as the process is both complex and time-consuming. In this study, an acetonitrile/tetrahydrofuran/di-(2-ethylhexyl) phosphoric acid/aqueous saturated sodium chloride solvent system was developed and utilized for the countercurrent chromatography of polar constituents from <i>Ginkgo biloba</i> L. seeds. Five polar constituents were effectively isolated using an acetonitrile/tetrahydrofuran/di-(2-ethylhexyl) phosphoric acid/aqueous saturated sodium chloride (2:2:0.8:3, <i>v</i>/<i>v</i>) solvent system using a two-step countercurrent chromatography method. In the initial countercurrent chromatography process, three constituents were successfully purified from the methanol extract: compound <b>1</b>, compound <b>4</b>, and compound <b>5</b>. Compounds <b>2</b> and <b>3</b>, co-eluted from the column, were further subjected to three inner-recycling chromatographic procedures. At last, five constituents were purified and identified, including 4′-O-methylpyridoxine (<b>1</b>); two indole alkaloid N-glucosides, ginkgoside B (<b>2</b>) and ginkgoside A (<b>3</b>); 2-(4-hydroxybenzyl) malic acid (<b>4</b>); and coniferyl alcohol (<b>5</b>). The results demonstrated that the acetonitrile/tetrahydrofuran/di-(2-ethylhexyl) phosphoric acid/aqueous saturated sodium chloride solvent system serves as a feasible system for the efficient countercurrent chromatography separation of polar components. |
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| ISSN: | 1420-3049 |