Validated Reverse-Phase High-Performance Liquid Chromatography for Quantification of Furosemide in Tablets and Nanoparticles
A simple, sensitive, and specific method for furosemide (FUR) analysis by reverse-phase-HPLC was developed using a Spherisorb C18 ODS 2 column. A chromatographic analysis was carried out using a mobile phase consisting of acetonitrile and 10 mM potassium phosphate buffer solution: 70 : 30 (v/v) at p...
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| Format: | Article |
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Wiley
2013-01-01
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| Series: | Journal of Analytical Methods in Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2013/207028 |
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| author | Ibrahima Youm Bi-Botti Celestin Youan |
| author_facet | Ibrahima Youm Bi-Botti Celestin Youan |
| author_sort | Ibrahima Youm |
| collection | DOAJ |
| description | A simple, sensitive, and specific method for furosemide (FUR) analysis by reverse-phase-HPLC was developed using a Spherisorb C18 ODS 2 column. A chromatographic analysis was carried out using a mobile phase consisting of acetonitrile and 10 mM potassium phosphate buffer solution: 70 : 30 (v/v) at pH 3.85, at a flow rate of 1 mL·min−1. The UV-detection method was carried out at 233 nm at room temperature. Validation parameters including limit of detection (LOD), limit of quantitation (LOQ), linearity range, precision, accuracy, robustness, and specificity were investigated. Results indicated that the calibration curve was linear () in the range of 5.2 to 25,000 ng·mL−1, with ε value equal to L·M−1·cm−1. The LOD and LOQ were found to be 5.2 and 15.8 ng·mL−1, respectively. The developed method was found to be accurate (RSD less than 2%), precise, and specific with an intraday and interday RSD range of 1.233–1.509 and 1.615 to 1.963%. The stability of native FUR has also been performed in simulated perilymph and endolymph media (with respective potency in each medium of % and %, ) after 6 hours. This method may be routinely used for the quantitative analysis of FUR from nanocarriers, USP tablets and release media related to hearing research |
| format | Article |
| id | doaj-art-f544c6369c9d41b9ab2cf0e441855f3c |
| institution | Kabale University |
| issn | 2090-8865 2090-8873 |
| language | English |
| publishDate | 2013-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Analytical Methods in Chemistry |
| spelling | doaj-art-f544c6369c9d41b9ab2cf0e441855f3c2025-02-03T01:01:51ZengWileyJournal of Analytical Methods in Chemistry2090-88652090-88732013-01-01201310.1155/2013/207028207028Validated Reverse-Phase High-Performance Liquid Chromatography for Quantification of Furosemide in Tablets and NanoparticlesIbrahima Youm0Bi-Botti Celestin Youan1Laboratory of Future Nanomedecines and Theoretical Chronopharmaceutics, Division of Pharmaceutical Sciences, University of Missouri, Kansas City, MO 64110, USALaboratory of Future Nanomedecines and Theoretical Chronopharmaceutics, Division of Pharmaceutical Sciences, University of Missouri, Kansas City, MO 64110, USAA simple, sensitive, and specific method for furosemide (FUR) analysis by reverse-phase-HPLC was developed using a Spherisorb C18 ODS 2 column. A chromatographic analysis was carried out using a mobile phase consisting of acetonitrile and 10 mM potassium phosphate buffer solution: 70 : 30 (v/v) at pH 3.85, at a flow rate of 1 mL·min−1. The UV-detection method was carried out at 233 nm at room temperature. Validation parameters including limit of detection (LOD), limit of quantitation (LOQ), linearity range, precision, accuracy, robustness, and specificity were investigated. Results indicated that the calibration curve was linear () in the range of 5.2 to 25,000 ng·mL−1, with ε value equal to L·M−1·cm−1. The LOD and LOQ were found to be 5.2 and 15.8 ng·mL−1, respectively. The developed method was found to be accurate (RSD less than 2%), precise, and specific with an intraday and interday RSD range of 1.233–1.509 and 1.615 to 1.963%. The stability of native FUR has also been performed in simulated perilymph and endolymph media (with respective potency in each medium of % and %, ) after 6 hours. This method may be routinely used for the quantitative analysis of FUR from nanocarriers, USP tablets and release media related to hearing researchhttp://dx.doi.org/10.1155/2013/207028 |
| spellingShingle | Ibrahima Youm Bi-Botti Celestin Youan Validated Reverse-Phase High-Performance Liquid Chromatography for Quantification of Furosemide in Tablets and Nanoparticles Journal of Analytical Methods in Chemistry |
| title | Validated Reverse-Phase High-Performance Liquid Chromatography for Quantification of Furosemide in Tablets and Nanoparticles |
| title_full | Validated Reverse-Phase High-Performance Liquid Chromatography for Quantification of Furosemide in Tablets and Nanoparticles |
| title_fullStr | Validated Reverse-Phase High-Performance Liquid Chromatography for Quantification of Furosemide in Tablets and Nanoparticles |
| title_full_unstemmed | Validated Reverse-Phase High-Performance Liquid Chromatography for Quantification of Furosemide in Tablets and Nanoparticles |
| title_short | Validated Reverse-Phase High-Performance Liquid Chromatography for Quantification of Furosemide in Tablets and Nanoparticles |
| title_sort | validated reverse phase high performance liquid chromatography for quantification of furosemide in tablets and nanoparticles |
| url | http://dx.doi.org/10.1155/2013/207028 |
| work_keys_str_mv | AT ibrahimayoum validatedreversephasehighperformanceliquidchromatographyforquantificationoffurosemideintabletsandnanoparticles AT bibotticelestinyouan validatedreversephasehighperformanceliquidchromatographyforquantificationoffurosemideintabletsandnanoparticles |