Effects of Soybean Isoflavones on the Growth Performance and Lipid Metabolism of the Juvenile Chinese Mitten Crab <i>Eriocheir sinensis</i>
In order to study the effects of soybean isoflavones on the growth performance and lipid metabolism of juvenile Chinese mitten crabs, six experimental diets were formulated by gradient supplementation with 0%, 0.004% and 0.008% soybean isoflavones at different dietary lipid levels (10% and 15%). The...
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| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2024-08-01
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| Series: | Fishes |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2410-3888/9/9/335 |
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| Summary: | In order to study the effects of soybean isoflavones on the growth performance and lipid metabolism of juvenile Chinese mitten crabs, six experimental diets were formulated by gradient supplementation with 0%, 0.004% and 0.008% soybean isoflavones at different dietary lipid levels (10% and 15%). The groups were named as follows: NF-0 group (10% fat and 0% SIFs), NF-0.004 group (10% fat and 0.004% SIFs), NF-0.008 group (10% fat and 0.008% SIFs), HF-0 group (15% fat and 0% SIFs), HF-0.004 group (15% fat and 0.004% SIFs) and HF-0.008 group (15% fat and 0.008% SIFs). All crabs with an initial weight of 0.4 ± 0.03 g were fed for 8 weeks. The results showed that dietary supplementation with 0.004% or 0.008% SIFs significantly increased the weight gain and specific growth rate of crabs. Diets supplemented with 0.004% or 0.008% SIFs significantly reduced the content of non-esterified free fatty acids and triglycerides in the hepatopancreas of crabs at the 10% dietary lipid level. Dietary SIFs significantly decreased the relative mRNA expressions of elongase of very-long-chain fatty acids 6 (<i>elovl6</i>), triglyceride lipase (<i>tgl</i>), sterol regulatory element-binding protein 1 (<i>srebp-1</i>), carnitine palmitoyltransferase-1a (<i>cpt-1a</i>), fatty acid transporter protein 4 (<i>fatp4</i>), carnitine palmitoyltransferase-2 (<i>cpt-2</i>), Δ9 fatty acyl desaturase (<i>Δ9 fad</i>), carnitine palmitoyltransferase-1b (<i>cpt-1b</i>), fatty acid-binding protein 10 <i>(fabp10</i>) and microsomal triglyceride transfer protein (<i>mttp</i>) in the hepatopancreas of crabs. At the 15% dietary lipid level, 0.008% SIFs significantly increased the relative mRNA expressions of fatty acid-binding protein 3 (<i>fabp3</i>), carnitine acetyltransferase (<i>caat</i>), <i>fatp4</i>, <i>fabp10</i>, <i>tgl</i>, <i>cpt-1a</i>, <i>cpt-1b</i> and <i>cpt-2</i> and significantly down-regulated the relative mRNA expressions of <i>Δ9 fad</i> and <i>srebp-1</i>. In conclusion, SIFs can improve the growth and utilization of a high-fat diet by inhibiting genes related to lipid synthesis and promoting lipid decomposition in juvenile Chinese mitten crabs. |
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| ISSN: | 2410-3888 |