Consumption of a High-Fat Diet Alters Perineuronal Nets in the Prefrontal Cortex
A key factor in the development of obesity is the overconsumption of fatty foods, which, in addition to facilitating weight gain, alters neuronal structures within brain reward circuitry. Our previous work demonstrates that sustained consumption of a high-fat diet (HFD) attenuates spine density in t...
Saved in:
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2018-01-01
|
| Series: | Neural Plasticity |
| Online Access: | http://dx.doi.org/10.1155/2018/2108373 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832562779989475328 |
|---|---|
| author | P. M. Dingess J. H. Harkness M. Slaker Z. Zhang S. S. Wulff B. A. Sorg T. E. Brown |
| author_facet | P. M. Dingess J. H. Harkness M. Slaker Z. Zhang S. S. Wulff B. A. Sorg T. E. Brown |
| author_sort | P. M. Dingess |
| collection | DOAJ |
| description | A key factor in the development of obesity is the overconsumption of fatty foods, which, in addition to facilitating weight gain, alters neuronal structures within brain reward circuitry. Our previous work demonstrates that sustained consumption of a high-fat diet (HFD) attenuates spine density in the prefrontal cortex (PFC). Whether HFD promotes structural adaptation among inhibitory cells of the PFC is presently unknown. One structure of interest is the perineuronal net (PNN), a specialized extracellular matrix surrounding, primarily, parvalbumin-containing GABAergic interneurons. PNNs contribute to synaptic stabilization, protect against oxidative stress, regulate the ionic microenvironment within cells, and modulate regional excitatory output. To examine diet-induced changes in PNNs, we maintained rats on one of three dietary conditions for 21 days: ad libitum chow, ad libitum 60% high fat (HF-AL), or limited-access calorically matched high fat (HF-CM), which produced no significant change in weight gain or adiposity with respect to chow controls. The PNN “number” and intensity were then quantified in the prelimbic (PL-PFC), infralimbic (IL-PFC), and ventral orbitofrontal cortex (OFC) using Wisteria floribunda agglutinin (WFA). Our results demonstrated that fat exposure, independent of weight gain, induced a robust decrease in the PNN intensity in the PL-PFC and OFC and a decrease in the PNN number in the OFC. |
| format | Article |
| id | doaj-art-f53969ccfba345e6b833eb0440aac830 |
| institution | Kabale University |
| issn | 2090-5904 1687-5443 |
| language | English |
| publishDate | 2018-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Neural Plasticity |
| spelling | doaj-art-f53969ccfba345e6b833eb0440aac8302025-02-03T01:21:56ZengWileyNeural Plasticity2090-59041687-54432018-01-01201810.1155/2018/21083732108373Consumption of a High-Fat Diet Alters Perineuronal Nets in the Prefrontal CortexP. M. Dingess0J. H. Harkness1M. Slaker2Z. Zhang3S. S. Wulff4B. A. Sorg5T. E. Brown6Neuroscience Program, University of Wyoming, Laramie, WY 82071, USADepartment of Integrative Physiology and Neuroscience, Washington State University, Vancouver, WA 98686, USADepartment of Pharmacology and Toxicology, Medical College of Wisconsin, Milwaukee, WI 53226, USADepartment of Zoology and Physiology, University of Wyoming, Laramie, WY 82071, USADepartment of Statistics, University of Wyoming, Laramie, WY 82071, USADepartment of Integrative Physiology and Neuroscience, Washington State University, Vancouver, WA 98686, USANeuroscience Program, University of Wyoming, Laramie, WY 82071, USAA key factor in the development of obesity is the overconsumption of fatty foods, which, in addition to facilitating weight gain, alters neuronal structures within brain reward circuitry. Our previous work demonstrates that sustained consumption of a high-fat diet (HFD) attenuates spine density in the prefrontal cortex (PFC). Whether HFD promotes structural adaptation among inhibitory cells of the PFC is presently unknown. One structure of interest is the perineuronal net (PNN), a specialized extracellular matrix surrounding, primarily, parvalbumin-containing GABAergic interneurons. PNNs contribute to synaptic stabilization, protect against oxidative stress, regulate the ionic microenvironment within cells, and modulate regional excitatory output. To examine diet-induced changes in PNNs, we maintained rats on one of three dietary conditions for 21 days: ad libitum chow, ad libitum 60% high fat (HF-AL), or limited-access calorically matched high fat (HF-CM), which produced no significant change in weight gain or adiposity with respect to chow controls. The PNN “number” and intensity were then quantified in the prelimbic (PL-PFC), infralimbic (IL-PFC), and ventral orbitofrontal cortex (OFC) using Wisteria floribunda agglutinin (WFA). Our results demonstrated that fat exposure, independent of weight gain, induced a robust decrease in the PNN intensity in the PL-PFC and OFC and a decrease in the PNN number in the OFC.http://dx.doi.org/10.1155/2018/2108373 |
| spellingShingle | P. M. Dingess J. H. Harkness M. Slaker Z. Zhang S. S. Wulff B. A. Sorg T. E. Brown Consumption of a High-Fat Diet Alters Perineuronal Nets in the Prefrontal Cortex Neural Plasticity |
| title | Consumption of a High-Fat Diet Alters Perineuronal Nets in the Prefrontal Cortex |
| title_full | Consumption of a High-Fat Diet Alters Perineuronal Nets in the Prefrontal Cortex |
| title_fullStr | Consumption of a High-Fat Diet Alters Perineuronal Nets in the Prefrontal Cortex |
| title_full_unstemmed | Consumption of a High-Fat Diet Alters Perineuronal Nets in the Prefrontal Cortex |
| title_short | Consumption of a High-Fat Diet Alters Perineuronal Nets in the Prefrontal Cortex |
| title_sort | consumption of a high fat diet alters perineuronal nets in the prefrontal cortex |
| url | http://dx.doi.org/10.1155/2018/2108373 |
| work_keys_str_mv | AT pmdingess consumptionofahighfatdietaltersperineuronalnetsintheprefrontalcortex AT jhharkness consumptionofahighfatdietaltersperineuronalnetsintheprefrontalcortex AT mslaker consumptionofahighfatdietaltersperineuronalnetsintheprefrontalcortex AT zzhang consumptionofahighfatdietaltersperineuronalnetsintheprefrontalcortex AT sswulff consumptionofahighfatdietaltersperineuronalnetsintheprefrontalcortex AT basorg consumptionofahighfatdietaltersperineuronalnetsintheprefrontalcortex AT tebrown consumptionofahighfatdietaltersperineuronalnetsintheprefrontalcortex |