Electroacupuncture suppresses NK cell infiltration and activation in the ischemic mouse brain through STAT3 inhibition
ABSTRACT: Aims: Electroacupuncture (EA) at Shuigou (GV26) and Baihui (GV20) has shown therapeutic benefits for stroke patients. Given that natural killer (NK) cell infiltration into the brain significantly contributes to the exacerbation of cerebral ischemic injury, this study investigated the impa...
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Elsevier
2024-12-01
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| Series: | Brain Research Bulletin |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0361923024002624 |
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| author | Ao Xu Ziqing Li Yangyang Ding Xiaoyu Wang Yufang Yang Lixia Du Deheng Wang Shi Shu Zhifei Wang |
| author_facet | Ao Xu Ziqing Li Yangyang Ding Xiaoyu Wang Yufang Yang Lixia Du Deheng Wang Shi Shu Zhifei Wang |
| author_sort | Ao Xu |
| collection | DOAJ |
| description | ABSTRACT: Aims: Electroacupuncture (EA) at Shuigou (GV26) and Baihui (GV20) has shown therapeutic benefits for stroke patients. Given that natural killer (NK) cell infiltration into the brain significantly contributes to the exacerbation of cerebral ischemic injury, this study investigated the impact of EA at Shuigou (GV26) and Baihui (GV20) on post-ischemic brain infiltration and activation of NK cells. Methods: Neurological deficit score, rotarod test, adhesive removal test, and TTC staining were used to evaluate the beneficial effects of EA in middle cerebral artery occlusion (MCAO) mice. The inhibitory effect of EA on STAT3 activation was assessed using Western blot. Flow cytometry was used to explore the impact of EA on post-ischemic brain infiltration of NK cells, as well as the activating receptor NKG2D expression and interferon-γ (IFN-γ) production by these infiltrated NK cells. Results: EA significantly alleviated neurological functional deficits and reduced brain infarction in MCAO mice. Abundant NK cells infiltrated into the ischemic hemisphere, but this infiltration was significantly suppressed by EA. Furthermore, EA attenuated NKG2D levels and reduced production of IFN-γ by NK cells in the ischemic brain. Notably, EA's inhibitory effect on post-ischemic NK cell brain infiltration and activation was comparable to that of STAT3 inhibition. The combination of EA and STAT3 inhibition did not result in further enhancement of the inhibitory effect. Moreover, the protective effects of EA against MCAO injury were abolished when STAT3 was activated. Conclusion: Our findings suggest that EA at Shuigou (GV26) and Baihui (GV20) inhibits the post-ischemic brain infiltration and activation of NK cells through STAT3 inhibition, significantly contributing to its therapeutic effects against brain ischemia. |
| format | Article |
| id | doaj-art-f5382056bb724d50a4a606f7929a57d3 |
| institution | Kabale University |
| issn | 1873-2747 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Brain Research Bulletin |
| spelling | doaj-art-f5382056bb724d50a4a606f7929a57d32024-11-28T04:34:06ZengElsevierBrain Research Bulletin1873-27472024-12-01219111128Electroacupuncture suppresses NK cell infiltration and activation in the ischemic mouse brain through STAT3 inhibitionAo Xu0Ziqing Li1Yangyang Ding2Xiaoyu Wang3Yufang Yang4Lixia Du5Deheng Wang6Shi Shu7Zhifei Wang8School of Integrative Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, ChinaSchool of Integrative Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China; Acupuncture and Moxibustion Department, Nanchang Hongdu Hospital of Traditional Chinese Medicine, Nanchang, Jiangxi 330008, ChinaSchool of Integrative Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, ChinaSchool of Integrative Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, ChinaSchool of Integrative Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, ChinaSchool of Integrative Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, ChinaSchool of Integrative Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, ChinaSchool of Traditional Chinese Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China; Corresponding authors.School of Integrative Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China; Corresponding authors.ABSTRACT: Aims: Electroacupuncture (EA) at Shuigou (GV26) and Baihui (GV20) has shown therapeutic benefits for stroke patients. Given that natural killer (NK) cell infiltration into the brain significantly contributes to the exacerbation of cerebral ischemic injury, this study investigated the impact of EA at Shuigou (GV26) and Baihui (GV20) on post-ischemic brain infiltration and activation of NK cells. Methods: Neurological deficit score, rotarod test, adhesive removal test, and TTC staining were used to evaluate the beneficial effects of EA in middle cerebral artery occlusion (MCAO) mice. The inhibitory effect of EA on STAT3 activation was assessed using Western blot. Flow cytometry was used to explore the impact of EA on post-ischemic brain infiltration of NK cells, as well as the activating receptor NKG2D expression and interferon-γ (IFN-γ) production by these infiltrated NK cells. Results: EA significantly alleviated neurological functional deficits and reduced brain infarction in MCAO mice. Abundant NK cells infiltrated into the ischemic hemisphere, but this infiltration was significantly suppressed by EA. Furthermore, EA attenuated NKG2D levels and reduced production of IFN-γ by NK cells in the ischemic brain. Notably, EA's inhibitory effect on post-ischemic NK cell brain infiltration and activation was comparable to that of STAT3 inhibition. The combination of EA and STAT3 inhibition did not result in further enhancement of the inhibitory effect. Moreover, the protective effects of EA against MCAO injury were abolished when STAT3 was activated. Conclusion: Our findings suggest that EA at Shuigou (GV26) and Baihui (GV20) inhibits the post-ischemic brain infiltration and activation of NK cells through STAT3 inhibition, significantly contributing to its therapeutic effects against brain ischemia.http://www.sciencedirect.com/science/article/pii/S0361923024002624ElectroacupunctureShuigou (GV26)Baihui (GV20)Ischemic strokeNatural killer cellsSignal transducer and activator of transcription 3 |
| spellingShingle | Ao Xu Ziqing Li Yangyang Ding Xiaoyu Wang Yufang Yang Lixia Du Deheng Wang Shi Shu Zhifei Wang Electroacupuncture suppresses NK cell infiltration and activation in the ischemic mouse brain through STAT3 inhibition Brain Research Bulletin Electroacupuncture Shuigou (GV26) Baihui (GV20) Ischemic stroke Natural killer cells Signal transducer and activator of transcription 3 |
| title | Electroacupuncture suppresses NK cell infiltration and activation in the ischemic mouse brain through STAT3 inhibition |
| title_full | Electroacupuncture suppresses NK cell infiltration and activation in the ischemic mouse brain through STAT3 inhibition |
| title_fullStr | Electroacupuncture suppresses NK cell infiltration and activation in the ischemic mouse brain through STAT3 inhibition |
| title_full_unstemmed | Electroacupuncture suppresses NK cell infiltration and activation in the ischemic mouse brain through STAT3 inhibition |
| title_short | Electroacupuncture suppresses NK cell infiltration and activation in the ischemic mouse brain through STAT3 inhibition |
| title_sort | electroacupuncture suppresses nk cell infiltration and activation in the ischemic mouse brain through stat3 inhibition |
| topic | Electroacupuncture Shuigou (GV26) Baihui (GV20) Ischemic stroke Natural killer cells Signal transducer and activator of transcription 3 |
| url | http://www.sciencedirect.com/science/article/pii/S0361923024002624 |
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